Ontology type: schema:ScholarlyArticle Open Access: True
2015-03
AUTHORSHiroaki Tanabe, Kanna Motoyama, Mariko Ikeda, Motoaki Wakiyama, Takaho Terada, Noboru Ohsawa, Toshiaki Hosaka, Masakatsu Hato, Yoshifumi Fujii, Yoshihiro Nakamura, Satoshi Ogasawara, Tomoya Hino, Takeshi Murata, So Iwata, Miki Okada-Iwabu, Masato Iwabu, Kunio Hirata, Yoshiaki Kawano, Masaki Yamamoto, Tomomi Kimura-Someya, Mikako Shirouzu, Toshimasa Yamauchi, Takashi Kadowaki, Shigeyuki Yokoyama
ABSTRACTThe adiponectin receptors (AdipoR1 and AdipoR2) are membrane proteins with seven transmembrane helices. These receptors regulate glucose and fatty acid metabolism, thereby ameliorating type 2 diabetes. The full-length human AdipoR1 and a series of N-terminally truncated mutants of human AdipoR1 and AdipoR2 were expressed in insect cells. In small-scale size exclusion chromatography, the truncated mutants AdipoR1Δ88 (residues 89-375) and AdipoR2Δ99 (residues 100-386) eluted mostly in the intact monodisperse state, while the others eluted primarily as aggregates. However, gel filtration chromatography of the large-scale preparation of the tag-affinity-purified AdipoR1Δ88 revealed the presence of an excessive amount of the aggregated state over the intact state. Since aggregation due to contaminating nucleic acids may have occurred during the sample concentration step, anion-exchange column chromatography was performed immediately after affinity chromatography, to separate the intact AdipoR1Δ88 from the aggregating species. The separated intact AdipoR1Δ88 did not undergo further aggregation, and was successfully purified to homogeneity by gel filtration chromatography. The purified AdipoR1Δ88 and AdipoR2Δ99 proteins were characterized by thermostability assays with 7-diethylamino-3-(4-maleimidophenyl)-4-methyl coumarin, thin layer chromatography of bound lipids, and surface plasmon resonance analysis of ligand binding, demonstrating their structural integrities. The AdipoR1Δ88 and AdipoR2Δ99 proteins were crystallized with the anti-AdipoR1 monoclonal antibody Fv fragment, by the lipidic mesophase method. X-ray diffraction data sets were obtained at resolutions of 2.8 and 2.4 Å, respectively. More... »
PAGES11-23
http://scigraph.springernature.com/pub.10.1007/s10969-014-9192-z
DOIhttp://dx.doi.org/10.1007/s10969-014-9192-z
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1051554476
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/25575462
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Amino Acid Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Blotting, Western",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cells, Cultured",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chromatography, Gel",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Crystallization",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Crystallography, X-Ray",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mutant Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mutation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Protein Aggregates",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Protein Binding",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Protein Stability",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Receptors, Adiponectin",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Sequence Homology, Amino Acid",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Surface Plasmon Resonance",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Temperature",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "X-Ray Diffraction",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Tokyo",
"id": "https://www.grid.ac/institutes/grid.26999.3d",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Department of Biophysics and Biochemistry and Laboratory of Structural Biology, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan",
"Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Tanabe",
"givenName": "Hiroaki",
"id": "sg:person.0604740544.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604740544.29"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Motoyama",
"givenName": "Kanna",
"id": "sg:person.0750460541.02",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750460541.02"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Ikeda",
"givenName": "Mariko",
"id": "sg:person.0734243370.36",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734243370.36"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Wakiyama",
"givenName": "Motoaki",
"id": "sg:person.01034032025.73",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034032025.73"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Terada",
"givenName": "Takaho",
"id": "sg:person.013626202062.24",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013626202062.24"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Ohsawa",
"givenName": "Noboru",
"id": "sg:person.01071370106.37",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071370106.37"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Hosaka",
"givenName": "Toshiaki",
"id": "sg:person.01213524105.96",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213524105.96"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Hato",
"givenName": "Masakatsu",
"id": "sg:person.01100236664.94",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100236664.94"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Fujii",
"givenName": "Yoshifumi",
"id": "sg:person.01266072544.73",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266072544.73"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Nakamura",
"givenName": "Yoshihiro",
"id": "sg:person.01040302123.92",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040302123.92"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Kyoto University",
"id": "https://www.grid.ac/institutes/grid.258799.8",
"name": [
"Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, 606-8501, Kyoto, Japan"
],
"type": "Organization"
},
"familyName": "Ogasawara",
"givenName": "Satoshi",
"id": "sg:person.01004221112.17",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01004221112.17"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Japan Science and Technology Agency",
"id": "https://www.grid.ac/institutes/grid.419082.6",
"name": [
"Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, 606-8501, Kyoto, Japan",
"JST, Research Acceleration Program, Membrane Protein Crystallography Project, Yoshida-Konoe-cho, Sakyo-ku, 606-8501, Kyoto, Japan"
],
"type": "Organization"
},
"familyName": "Hino",
"givenName": "Tomoya",
"id": "sg:person.0626523575.13",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626523575.13"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Chiba University",
"id": "https://www.grid.ac/institutes/grid.136304.3",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, 606-8501, Kyoto, Japan",
"JST, Research Acceleration Program, Membrane Protein Crystallography Project, Yoshida-Konoe-cho, Sakyo-ku, 606-8501, Kyoto, Japan",
"Department of Chemistry, Graduate School of Science, Chiba University, Yayoi-cho, Inage, 263-8522, Chiba, Japan"
],
"type": "Organization"
},
"familyName": "Murata",
"givenName": "Takeshi",
"id": "sg:person.01370160306.34",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370160306.34"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Diamond Light Source (United Kingdom)",
"id": "https://www.grid.ac/institutes/grid.18785.33",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, 606-8501, Kyoto, Japan",
"JST, Research Acceleration Program, Membrane Protein Crystallography Project, Yoshida-Konoe-cho, Sakyo-ku, 606-8501, Kyoto, Japan",
"Division of Molecular Biosciences, Membrane Protein Crystallography Group, Imperial College, SW7 2AZ, London, UK",
"Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Didcot, OX11 0DE, Oxfordshire, UK",
"RIKEN SPring-8 Center, Harima Institute, Kouto, 679-5148, Sayo, Hyogo, Japan"
],
"type": "Organization"
},
"familyName": "Iwata",
"givenName": "So",
"id": "sg:person.01337410152.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337410152.19"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Tokyo",
"id": "https://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan",
"Department of Integrated Molecular Science on Metabolic Diseases, 22nd Century Medical and Research Center, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Okada-Iwabu",
"givenName": "Miki",
"id": "sg:person.01004767325.20",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01004767325.20"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Japan Science and Technology Agency",
"id": "https://www.grid.ac/institutes/grid.419082.6",
"name": [
"Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan",
"Department of Integrated Molecular Science on Metabolic Diseases, 22nd Century Medical and Research Center, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan",
"PRESTO, Japan Science and Technology Agency, 332-0012, Kawaguchi, Saitama, Japan"
],
"type": "Organization"
},
"familyName": "Iwabu",
"givenName": "Masato",
"id": "sg:person.01346102065.94",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346102065.94"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"RIKEN SPring-8 Center, Harima Institute, Kouto, 679-5148, Sayo, Hyogo, Japan"
],
"type": "Organization"
},
"familyName": "Hirata",
"givenName": "Kunio",
"id": "sg:person.01256470305.03",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256470305.03"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"RIKEN SPring-8 Center, Harima Institute, Kouto, 679-5148, Sayo, Hyogo, Japan"
],
"type": "Organization"
},
"familyName": "Kawano",
"givenName": "Yoshiaki",
"id": "sg:person.0756317004.75",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756317004.75"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"RIKEN SPring-8 Center, Harima Institute, Kouto, 679-5148, Sayo, Hyogo, Japan"
],
"type": "Organization"
},
"familyName": "Yamamoto",
"givenName": "Masaki",
"id": "sg:person.012771421304.81",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012771421304.81"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Kimura-Someya",
"givenName": "Tomomi",
"id": "sg:person.01104104440.76",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104104440.76"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "RIKEN",
"id": "https://www.grid.ac/institutes/grid.7597.c",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Shirouzu",
"givenName": "Mikako",
"id": "sg:person.01274360652.26",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274360652.26"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Japan Science and Technology Agency",
"id": "https://www.grid.ac/institutes/grid.419082.6",
"name": [
"Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan",
"Department of Integrated Molecular Science on Metabolic Diseases, 22nd Century Medical and Research Center, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan",
"CREST, Japan Science and Technology Agency, 332-0012, Kawaguchi, Saitama, Japan"
],
"type": "Organization"
},
"familyName": "Yamauchi",
"givenName": "Toshimasa",
"id": "sg:person.012027672272.37",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012027672272.37"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Tokyo",
"id": "https://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan",
"Department of Integrated Molecular Science on Metabolic Diseases, 22nd Century Medical and Research Center, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Kadowaki",
"givenName": "Takashi",
"id": "sg:person.01353472360.41",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01353472360.41"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Tokyo",
"id": "https://www.grid.ac/institutes/grid.26999.3d",
"name": [
"RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan",
"Department of Biophysics and Biochemistry and Laboratory of Structural Biology, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan",
"RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Japan"
],
"type": "Organization"
},
"familyName": "Yokoyama",
"givenName": "Shigeyuki",
"id": "sg:person.0723444525.34",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723444525.34"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1006/bbrc.1996.0587",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000410466"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/256495a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001387921",
"https://doi.org/10.1038/256495a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0076-6879(97)76066-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004907170"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.222657499",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005787192"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.str.2008.02.004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005996447"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/bioinformatics/btm404",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007683223"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35013000",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007859471",
"https://doi.org/10.1038/35013000"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35013000",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007859471",
"https://doi.org/10.1038/35013000"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature10361",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008768060",
"https://doi.org/10.1038/nature10361"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.str.2010.12.001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011130886"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1107/s0021889807021206",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012753217"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature08991",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013311707",
"https://doi.org/10.1038/nature08991"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature08991",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013311707",
"https://doi.org/10.1038/nature08991"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.bbrc.2012.12.107",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020813213"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1194/jlr.d400022-jlr200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022647926"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/b:jsfg.0000029204.57846.7d",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022949189",
"https://doi.org/10.1023/b:jsfg.0000029204.57846.7d"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10969-007-9038-z",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028661600",
"https://doi.org/10.1007/s10969-007-9038-z"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1161/01.atv.20.6.1595",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029685152"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1107/s0907444909047337",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032162623"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1107/s0907444909047337",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032162623"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cmet.2004.12.003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033736922"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0076-6879(03)72004-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034856067"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m209033200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035029420"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.271.18.10697",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035318566"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.str.2008.05.001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035639709"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.270.45.26746",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035808233"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10969-014-9187-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035811374",
"https://doi.org/10.1007/s10969-014-9187-9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10969-014-9187-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035811374",
"https://doi.org/10.1007/s10969-014-9187-9"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/bioinformatics/17.9.849",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038106723"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1088/1742-6596/425/1/012002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044156712"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nm788",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045145719",
"https://doi.org/10.1038/nm788"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nm788",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045145719",
"https://doi.org/10.1038/nm788"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/376660a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045500886",
"https://doi.org/10.1038/376660a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3802/jgo.2012.23.3.153",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046965904"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature10750",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047808727",
"https://doi.org/10.1038/nature10750"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1107/s0909049505004735",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050248304"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.sbi.2013.04.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050578818"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.sbi.2013.04.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050578818"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.sbi.2013.04.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050578818"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.sbi.2013.04.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050578818"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.sbi.2013.04.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050578818"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature01705",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051052427",
"https://doi.org/10.1038/nature01705"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature01705",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051052427",
"https://doi.org/10.1038/nature01705"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkt381",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051947060"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/jp902883q",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056114777"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/jp902883q",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056114777"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/oxfordjournals.jbchem.a021483",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059960285"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1076794360",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1096/fasebj.11.5.9141501",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083085891"
],
"type": "CreativeWork"
}
],
"datePublished": "2015-03",
"datePublishedReg": "2015-03-01",
"description": "The adiponectin receptors (AdipoR1 and AdipoR2) are membrane proteins with seven transmembrane helices. These receptors regulate glucose and fatty acid metabolism, thereby ameliorating type 2 diabetes. The full-length human AdipoR1 and a series of N-terminally truncated mutants of human AdipoR1 and AdipoR2 were expressed in insect cells. In small-scale size exclusion chromatography, the truncated mutants AdipoR1\u039488 (residues 89-375) and AdipoR2\u039499 (residues 100-386) eluted mostly in the intact monodisperse state, while the others eluted primarily as aggregates. However, gel filtration chromatography of the large-scale preparation of the tag-affinity-purified AdipoR1\u039488 revealed the presence of an excessive amount of the aggregated state over the intact state. Since aggregation due to contaminating nucleic acids may have occurred during the sample concentration step, anion-exchange column chromatography was performed immediately after affinity chromatography, to separate the intact AdipoR1\u039488 from the aggregating species. The separated intact AdipoR1\u039488 did not undergo further aggregation, and was successfully purified to homogeneity by gel filtration chromatography. The purified AdipoR1\u039488 and AdipoR2\u039499 proteins were characterized by thermostability assays with 7-diethylamino-3-(4-maleimidophenyl)-4-methyl coumarin, thin layer chromatography of bound lipids, and surface plasmon resonance analysis of ligand binding, demonstrating their structural integrities. The AdipoR1\u039488 and AdipoR2\u039499 proteins were crystallized with the anti-AdipoR1 monoclonal antibody Fv fragment, by the lipidic mesophase method. X-ray diffraction data sets were obtained at resolutions of 2.8 and 2.4\u00a0\u00c5, respectively. ",
"genre": "research_article",
"id": "sg:pub.10.1007/s10969-014-9192-z",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.6146616",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.6129108",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.6128974",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.6138529",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1029935",
"issn": [
"1345-711X",
"1570-0267"
],
"name": "Journal of Structural and Functional Genomics",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "16"
}
],
"name": "Expression, purification, crystallization, and preliminary X-ray crystallographic studies of the human adiponectin receptors, AdipoR1 and AdipoR2",
"pagination": "11-23",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"f595277f5c2dba68eed0566d1d6e6162bfd705dd43eedac4f4f9d3e419729487"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"25575462"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"101128185"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s10969-014-9192-z"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1051554476"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s10969-014-9192-z",
"https://app.dimensions.ai/details/publication/pub.1051554476"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T13:12",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000367_0000000367/records_88257_00000001.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007%2Fs10969-014-9192-z"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10969-014-9192-z'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10969-014-9192-z'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10969-014-9192-z'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10969-014-9192-z'
This table displays all metadata directly associated to this object as RDF triples.
483 TRIPLES
21 PREDICATES
87 URIs
41 LITERALS
29 BLANK NODES