A new manual dispensing system for in meso membrane protein crystallization with using a stepping motor-based dispenser View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-09

AUTHORS

Masakatsu Hato, Toshiaki Hosaka, Hiroaki Tanabe, Tokuji Kitsunai, Shigeyuki Yokoyama

ABSTRACT

A reliable and easy to use manual dispensing system has been developed for the in meso membrane protein crystallization method. The system consists of a stepping motor-based dispenser with a new microsyringe system for dispensing, which allows us to deliver any desired volume of highly viscous lipidic mesophase in the range from ~50 to at least ~200 nl. The average, standard deviation, and coefficient of variation of 20 repeated deliveries of 50 nl cubic phase were comparable to those of a current robotic dispensing. Moreover, the bottom faces of boluses delivered to the glass crystallization plate were reproducibly circular in shape, and their centers were within about 100 μm from the center of the crystallization well. The system was useful for crystallizing membrane and soluble proteins in meso. More... »

PAGES

165-171

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10969-014-9187-9

DOI

http://dx.doi.org/10.1007/s10969-014-9187-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035811374

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25056837


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