Preparation and biological evaluation of radiogallium labeled glucagon for SPECT imaging View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-09

AUTHORS

Amir Reza Jalilian, Mahdokht Jouiaei, Alireza Doroudi, Javad Garousi, Sedigheh Moradkhani

ABSTRACT

In this work, recently prepared 67Ga-labeled glucagon (67Ga-DTPA-GCG) for imaging studies (radiochemical purity >94%; HPLC, S.A. 296–370 GBq/mM) was used in biological studies. The wild-type rat biodistribution results, 2 h post injection, demonstrated high tissue:muscle ratios for target tissues (liver, kidney, heart, spleen, fat intestine stomach and pancreas), 234, 18.45, 7.12, 1.75, 128.7, 4.9, 6.3 and 1.11, respectively. The tracer binding capacity using freshly prepared rat brain homogenate demonstrated significant specific binding of the tracer to neuronal GCG receptors (67Ga-DTPA-GCG/67Ga:3 and 67Ga-DTPA-GCG/67GaDTPA:2.2 at 90 min). SPECT images also demonstrated target specific binding of the tracer at 4 h. The data suggests the tracer is accumulated in GCGR rich tissues 2–4 h post injection, suggesting potentials of the tracer for future imaging studies in glocagonoma models. More... »

PAGES

555-561

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10967-010-0555-z

DOI

http://dx.doi.org/10.1007/s10967-010-0555-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001584079


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