Macroporous 2-hydroxyethyl methacrylate hydrogels of dual porosity for cell cultivation: morphology, swelling, permeability, and mechanical behavior View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-11

AUTHORS

Martin Přádný, Miroslava Dušková-Smrčková, Karel Dušek, Olga Janoušková, Zhansaya Sadakbayeva, Miroslav Šlouf, Jiří Michálek

ABSTRACT

Macroporous hydrogels of dual porosity based on cross-linked poly(2-hydroxyethyl methacrylate) (pHEMA) were prepared, and their swelling, mechanical responses, and hydraulic permeability, as well as cell adhesion and proliferation, were examined on gel-based scaffolds. Large pores on the order of tens and hundreds of microns were generated by adding a solid porogen (fractionated particles of sodium chloride) to the polymerization mixture, which was subsequently washed out of the gel. Small pores on the order of a few microns were formed by the reaction-induced phase separation mechanism caused by addition of a diluent (1-dodecanol), which is a poor solvent for pHEMA. Morphological studies using light and electron microscopy techniques revealed that the large pores were embedded in the pHEMA matrix containing small fused spherical pores. Mechanical behavior of the dual-porosity hydrogels characterized by oscillatory shear measurements revealed that despite very high pore volume, the hydrogels were sufficiently stiff and self-supportive. The cell cultivation pilot experiment showed that the morphology (dual porosity) demonstrated marked effects on the promotion of cell adhesion, growth, and proliferation, not only on the gel surface but in the large pores within the gel bulk as well. Graphical AbstractDual porosity hydrogel was formed using washable templates (large pores) and utilizing the phase separation within the hydrogel walls (fine porosity) in one step. The hydrogel structure was visualized by scanning electron microscopy (gel without cells) - the image confirmed dual porosity. Dual porosity hydrogel was formed using washable templates (large pores) and utilizing the phase separation within the hydrogel walls (fine porosity) in one step. The hydrogel structure was visualized by scanning electron microscopy (gel without cells) - the image confirmed dual porosity. More... »

PAGES

579

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10965-014-0579-0

DOI

http://dx.doi.org/10.1007/s10965-014-0579-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013920887


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RDF/XML is a standard XML format for linked data.

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This table displays all metadata directly associated to this object as RDF triples.

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