Critical Role of Zinc Ion on E. coli Glutamyl-Queuosine-tRNAAsp Synthetase (Glu-Q-RS) Structure and Function View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-04

AUTHORS

Sutapa Ray, Victor Banerjee, Mickael Blaise, Baisakhi Banerjee, Kali Pada Das, Daniel Kern, Rajat Banerjee

ABSTRACT

Glutamyl-queuosine-tRNA(Asp) synthetase (Glu-Q-RS) and glutamyl-tRNA synthetase (GluRS), differ widely by their function although they share close structural resemblance within their catalytic core of GluRS. In particular both Escherichia coli GluRS and Glu-Q-RS contain a single zinc-binding site in their putative tRNA acceptor stem-binding domain. It has been shown that the zinc is crucial for correct positioning of the tRNA(Glu) acceptor-end in the active site of E. coli GluRS. To address the role of zinc ion in Glu-Q-RS, the C101S/C103S Glu-Q-RS variant is constructed. Energy dispersive X-ray fluorescence show that the zinc ion still remained coordinated but the variant became structurally labile and acquired aggregation capacity. The extent of aggregation of the protein is significantly decreased in presence of the small substrates and more particularly by adenosine triphosphate. Addition of zinc increased significantly the solubility of the variant. The aminoacylation assay reveals a decrease in activity of the variant even after addition of zinc as compared to the wild-type, although the secondary structure of the protein is not altered as shown by the Fourier transform infrared spectroscopy study. More... »

PAGES

143-149

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10930-014-9546-1

DOI

http://dx.doi.org/10.1007/s10930-014-9546-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048819512

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24505021


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