An Arsenic Fluorescent Compound as a Novel Probe to Study Arsenic-Binding Proteins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-08-31

AUTHORS

A. Lis Femia, C. Facundo Temprana, Javier Santos, María Laura Carbajal, María Silvia Amor, Mariano Grasselli, Silvia del V. Alonso

ABSTRACT

Arsenic-binding proteins are under continuous research. Their identification and the elucidation of arsenic/protein interaction mechanisms are important because the biological effects of these complexes may be related not only to arsenic but also to the arsenic/protein structure. Although many proteins bearing a CXXC motif have been found to bind arsenic in vivo, new tools are necessary to identify new arsenic targets and allow research on protein/arsenic complexes. In this work, we analyzed the performance of the fluorescent compound APAO-FITC (synthesized from p-aminophenylarsenoxide, APAO, and fluorescein isothiocyanate, FITC) in arsenic/protein binding assays using thioredoxin 1 (Trx) as an arsenic-binding protein model. The Trx-APAO-FITC complex was studied through different spectroscopic techniques involving UV–Vis, fluorescence, atomic absorption, infrared and circular dichroism. Our results show that APAO-FITC binds efficiently and specifically to the Trx binding site, labeling the protein fluorescently, without altering its structure and activity. In summary, we were able to study a protein/arsenic complex model, using APAO-FITC as a labeling probe. The use of APAO-FITC in the identification of different protein and cell targets, as well as in in vivo biodistribution studies, conformational studies of arsenic-binding proteins, and studies for the design of drug delivery systems for arsenic anti-cancer therapies, is highly promising. More... »

PAGES

656-666

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10930-012-9441-6

DOI

http://dx.doi.org/10.1007/s10930-012-9441-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000396197

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22936492


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