Ontology type: schema:ScholarlyArticle Open Access: True
2011-12-02
AUTHORSAnkit Singh, Dinesh Yadav, Krishan Mohan Rai, Meenal Srivastava, Praveen C. Verma, Pradhyumna K. Singh, Rakesh Tuli
ABSTRACTFusion systems are known to increase the expression of difficult to express recombinant proteins in soluble form to facilitate their purification. Rabies glycoprotein was also tough to express at sufficient level in soluble form in both E. coli and plant. The present work was aimed to over-express and purify this membrane protein from soluble extract of E. coli. Fusion of Small Ubiqutin like Modifier (SUMO) with rabies glycoprotein increased ~1.5 fold higher expression and ~3.0 fold solubility in comparison to non-fused in E. coli. The SUMO fusion also simplified the purification process. Previously engineered rabies glycoprotein gene in tobacco plants provides complete protection to mice, but the expression was very low for purification. Our finding demonstrated that the SUMO-fusion was useful for enhancing expression and solubility of the membrane protein and again proves to be a good alternative technology for applications in biomedical and pharmaceutical research. More... »
PAGES68-74
http://scigraph.springernature.com/pub.10.1007/s10930-011-9373-6
DOIhttp://dx.doi.org/10.1007/s10930-011-9373-6
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1014482769
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/22134654
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Escherichia coli",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, Inbred BALB C",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rabies virus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Recombinant Fusion Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Small Ubiquitin-Related Modifier Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Viral Envelope Proteins",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India"
],
"type": "Organization"
},
"familyName": "Singh",
"givenName": "Ankit",
"id": "sg:person.0601114063.38",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601114063.38"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India"
],
"type": "Organization"
},
"familyName": "Yadav",
"givenName": "Dinesh",
"id": "sg:person.01176615401.43",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01176615401.43"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India"
],
"type": "Organization"
},
"familyName": "Rai",
"givenName": "Krishan Mohan",
"id": "sg:person.0702374573.93",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702374573.93"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India"
],
"type": "Organization"
},
"familyName": "Srivastava",
"givenName": "Meenal",
"id": "sg:person.0610743561.74",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610743561.74"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India"
],
"type": "Organization"
},
"familyName": "Verma",
"givenName": "Praveen C.",
"id": "sg:person.0635677533.38",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635677533.38"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"Plant Molecular Biology & Genetic Engineering, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, 226001, Lucknow, UP, India"
],
"type": "Organization"
},
"familyName": "Singh",
"givenName": "Pradhyumna K.",
"id": "sg:person.013250060462.03",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013250060462.03"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Agri-Food Biotechnology Institute, (DBT, New Delhi), C-127, Phase VIII, Industrial Area, SAS Nagar, 160071, Mohali, Punjab, India",
"id": "http://www.grid.ac/institutes/grid.452674.6",
"name": [
"National Agri-Food Biotechnology Institute, (DBT, New Delhi), C-127, Phase VIII, Industrial Area, SAS Nagar, 160071, Mohali, Punjab, India"
],
"type": "Organization"
},
"familyName": "Tuli",
"givenName": "Rakesh",
"id": "sg:person.0715545447.89",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715545447.89"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s10969-005-2664-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030509721",
"https://doi.org/10.1007/s10969-005-2664-4"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/b:jsfg.0000029237.70316.52",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052800866",
"https://doi.org/10.1023/b:jsfg.0000029237.70316.52"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1295-1484",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019265871",
"https://doi.org/10.1038/nbt1295-1484"
],
"type": "CreativeWork"
}
],
"datePublished": "2011-12-02",
"datePublishedReg": "2011-12-02",
"description": "Fusion systems are known to increase the expression of difficult to express recombinant proteins in soluble form to facilitate their purification. Rabies glycoprotein was also tough to express at sufficient level in soluble form in both E. coli and plant. The present work was aimed to over-express and purify this membrane protein from soluble extract of E. coli. Fusion of Small Ubiqutin like Modifier (SUMO) with rabies glycoprotein increased ~1.5 fold higher expression and ~3.0 fold solubility in comparison to non-fused in E. coli. The SUMO fusion also simplified the purification process. Previously engineered rabies glycoprotein gene in tobacco plants provides complete protection to mice, but the expression was very low for purification. Our finding demonstrated that the SUMO-fusion was useful for enhancing expression and solubility of the membrane protein and again proves to be a good alternative technology for applications in biomedical and pharmaceutical research.",
"genre": "article",
"id": "sg:pub.10.1007/s10930-011-9373-6",
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1297375",
"issn": [
"1572-3887",
"1573-4943"
],
"name": "The Protein Journal",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "31"
}
],
"keywords": [
"membrane proteins",
"SUMO fusion",
"E. coli",
"surface G-protein",
"tobacco plants",
"soluble form",
"rabies glycoprotein gene",
"recombinant proteins",
"G proteins",
"glycoprotein gene",
"Escherichia coli",
"coli",
"protein",
"enhanced expression",
"high expression",
"expression",
"plants",
"soluble extracts",
"fusion",
"purification",
"genes",
"pharmaceutical research",
"good alternative technology",
"sufficient level",
"fusion system",
"complete protection",
"mice",
"purification process",
"modifiers",
"extract",
"form",
"rabies",
"levels",
"present work",
"protection",
"process",
"findings",
"comparison",
"system",
"alternative technologies",
"solubility",
"research",
"work",
"technology",
"applications"
],
"name": "Enhanced Expression of Rabies Virus Surface G-Protein in Escherichia coli using SUMO Fusion",
"pagination": "68-74",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1014482769"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s10930-011-9373-6"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"22134654"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s10930-011-9373-6",
"https://app.dimensions.ai/details/publication/pub.1014482769"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:59",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_551.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s10930-011-9373-6"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10930-011-9373-6'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10930-011-9373-6'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10930-011-9373-6'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10930-011-9373-6'
This table displays all metadata directly associated to this object as RDF triples.
203 TRIPLES
21 PREDICATES
83 URIs
72 LITERALS
17 BLANK NODES