Mutagenesis Studies on the N-terminus and Thr54 of Naja naja atra (Taiwan cobra) Chymotrypsin Inhibitor View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-05-16

AUTHORS

Fang-Jiun Yan, Ching-Ping Chen, Yun-Ching Cheng, Long-Sen Chang

ABSTRACT

Ala-screening mutagenesis studies on Arg1, Pro2, Arg3, Phe4 and Thr54 of Naja naja atra (Taiwan cobra) chymotrypsin inhibitor showed that inhibitory potency and gross conformation of the mutants were not significantly different from those of wild-type inhibitor. Nevertheless, the R1A mutant had an appreciable decrease in the structural stability underlying thermal unfolding and urea-induced denaturation. Alternatively, deleting the first three residues at the N-terminus caused a reduction in structural stability as well as inhibitory potency. In sharp contrast to wild-type and other mutated inhibitors, R1A mutant and truncated mutant completely lost their inhibitory activity when the inhibitors were incubated with chymotrypsin for periods of up to 3 h. The loss of activity correlated with chymotryptic cleavage of inhibitors as evidenced by SDA-PAGE. Taken together, these results reflect that the globally structural rigidity of N. naja atra chymotrypsin inhibitor functionally affects the sustainable period in inhibiting chymotrypsin activity, and that the intact N-terminus might contribute to this event. More... »

PAGES

257-262

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10930-006-9009-4

DOI

http://dx.doi.org/10.1007/s10930-006-9009-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044720605

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16703468


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Substitution", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chymotrypsin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Elapid Venoms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Elapidae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Taiwan", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan", 
          "id": "http://www.grid.ac/institutes/grid.412036.2", 
          "name": [
            "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yan", 
        "givenName": "Fang-Jiun", 
        "id": "sg:person.01234357506.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234357506.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan", 
          "id": "http://www.grid.ac/institutes/grid.412036.2", 
          "name": [
            "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Ching-Ping", 
        "id": "sg:person.0625637355.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625637355.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan", 
          "id": "http://www.grid.ac/institutes/grid.412036.2", 
          "name": [
            "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cheng", 
        "givenName": "Yun-Ching", 
        "id": "sg:person.0716147312.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716147312.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan", 
          "id": "http://www.grid.ac/institutes/grid.412036.2", 
          "name": [
            "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chang", 
        "givenName": "Long-Sen", 
        "id": "sg:person.0770353614.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770353614.14"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf01024879", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026609357", 
          "https://doi.org/10.1007/bf01024879"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-05-16", 
    "datePublishedReg": "2006-05-16", 
    "description": "Ala-screening mutagenesis studies on Arg1, Pro2, Arg3, Phe4 and Thr54 of Naja naja atra (Taiwan cobra) chymotrypsin inhibitor showed that inhibitory potency and gross conformation of the mutants were not significantly different from those of wild-type inhibitor. Nevertheless, the R1A mutant had an appreciable decrease in the structural stability underlying thermal unfolding and urea-induced denaturation. Alternatively, deleting the first three residues at the N-terminus caused a reduction in structural stability as well as inhibitory potency. In sharp contrast to wild-type and other mutated inhibitors, R1A mutant and truncated mutant completely lost their inhibitory activity when the inhibitors were incubated with chymotrypsin for periods of up to 3\u00a0h. The loss of activity correlated with chymotryptic cleavage of inhibitors as evidenced by SDA-PAGE. Taken together, these results reflect that the globally structural rigidity of N. naja atra chymotrypsin inhibitor functionally affects the sustainable period in inhibiting chymotrypsin activity, and that the intact N-terminus might contribute to this event.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s10930-006-9009-4", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297375", 
        "issn": [
          "1572-3887", 
          "1573-4943"
        ], 
        "name": "The Protein Journal", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "25"
      }
    ], 
    "keywords": [
      "N-terminus", 
      "chymotrypsin inhibitor", 
      "mutagenesis studies", 
      "wild-type inhibitor", 
      "intact N-terminus", 
      "mutants", 
      "SDA-PAGE", 
      "thermal unfolding", 
      "gross conformation", 
      "inhibitory potency", 
      "chymotryptic cleavage", 
      "chymotrypsin activity", 
      "loss of activity", 
      "inhibitors", 
      "Thr54", 
      "Arg3", 
      "structural stability", 
      "residues", 
      "inhibitory activity", 
      "activity", 
      "structural rigidity", 
      "Ala", 
      "chymotrypsin", 
      "cleavage", 
      "unfolding", 
      "conformation", 
      "denaturation", 
      "potency", 
      "sharp contrast", 
      "Arg1", 
      "Phe4", 
      "Pro2", 
      "contrast", 
      "sustainable period", 
      "loss", 
      "events", 
      "study", 
      "appreciable decrease", 
      "stability", 
      "decrease", 
      "period", 
      "results", 
      "reduction", 
      "rigidity"
    ], 
    "name": "Mutagenesis Studies on the N-terminus and Thr54 of Naja naja atra (Taiwan cobra) Chymotrypsin Inhibitor", 
    "pagination": "257-262", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1044720605"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10930-006-9009-4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16703468"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10930-006-9009-4", 
      "https://app.dimensions.ai/details/publication/pub.1044720605"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:33", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_413.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s10930-006-9009-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10930-006-9009-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10930-006-9009-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10930-006-9009-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10930-006-9009-4'


 

This table displays all metadata directly associated to this object as RDF triples.

162 TRIPLES      21 PREDICATES      78 URIs      69 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10930-006-9009-4 schema:about N09bd1146e84245b09d21009f8cc7fd62
2 N0ea37ccc245d43f98a9484da4af44ba3
3 N2e4f88d7d5234f988af3b83dc31f2793
4 N364ad02aa2e24d42a0abcee40307ea9a
5 N74279ee9d2d04694b3359d5ba5224b01
6 N7a8ef6bcfc9b4bc8828eacb9a5cff95d
7 N8f78c31dbcf247c2a8226a76273fe688
8 Ne44af95b36e84078ac10243dc0dd550f
9 anzsrc-for:06
10 anzsrc-for:0601
11 schema:author Ne028b211c150422ea93dc03f87693c48
12 schema:citation sg:pub.10.1007/bf01024879
13 schema:datePublished 2006-05-16
14 schema:datePublishedReg 2006-05-16
15 schema:description Ala-screening mutagenesis studies on Arg1, Pro2, Arg3, Phe4 and Thr54 of Naja naja atra (Taiwan cobra) chymotrypsin inhibitor showed that inhibitory potency and gross conformation of the mutants were not significantly different from those of wild-type inhibitor. Nevertheless, the R1A mutant had an appreciable decrease in the structural stability underlying thermal unfolding and urea-induced denaturation. Alternatively, deleting the first three residues at the N-terminus caused a reduction in structural stability as well as inhibitory potency. In sharp contrast to wild-type and other mutated inhibitors, R1A mutant and truncated mutant completely lost their inhibitory activity when the inhibitors were incubated with chymotrypsin for periods of up to 3 h. The loss of activity correlated with chymotryptic cleavage of inhibitors as evidenced by SDA-PAGE. Taken together, these results reflect that the globally structural rigidity of N. naja atra chymotrypsin inhibitor functionally affects the sustainable period in inhibiting chymotrypsin activity, and that the intact N-terminus might contribute to this event.
16 schema:genre article
17 schema:isAccessibleForFree false
18 schema:isPartOf N31b31795d887466e998c0c6ea35752f8
19 N6bfc776a043a429499f2cb8a8e9c8269
20 sg:journal.1297375
21 schema:keywords Ala
22 Arg1
23 Arg3
24 N-terminus
25 Phe4
26 Pro2
27 SDA-PAGE
28 Thr54
29 activity
30 appreciable decrease
31 chymotrypsin
32 chymotrypsin activity
33 chymotrypsin inhibitor
34 chymotryptic cleavage
35 cleavage
36 conformation
37 contrast
38 decrease
39 denaturation
40 events
41 gross conformation
42 inhibitors
43 inhibitory activity
44 inhibitory potency
45 intact N-terminus
46 loss
47 loss of activity
48 mutagenesis studies
49 mutants
50 period
51 potency
52 reduction
53 residues
54 results
55 rigidity
56 sharp contrast
57 stability
58 structural rigidity
59 structural stability
60 study
61 sustainable period
62 thermal unfolding
63 unfolding
64 wild-type inhibitor
65 schema:name Mutagenesis Studies on the N-terminus and Thr54 of Naja naja atra (Taiwan cobra) Chymotrypsin Inhibitor
66 schema:pagination 257-262
67 schema:productId N18600874616848f987f78666076d5422
68 N2f6f3c24efb04bc2b470ebfe0cd979c8
69 Nf155d0b23d9a4d19919cc37cbeb35098
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044720605
71 https://doi.org/10.1007/s10930-006-9009-4
72 schema:sdDatePublished 2022-10-01T06:33
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher N2dd9ee9747be428fb1b18d2ee1778730
75 schema:url https://doi.org/10.1007/s10930-006-9009-4
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N059282d4fcc34408afb734a1830fc602 rdf:first sg:person.0716147312.76
80 rdf:rest N9a5ba94c645842f3a537c9170440ef24
81 N09bd1146e84245b09d21009f8cc7fd62 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Chymotrypsin
83 rdf:type schema:DefinedTerm
84 N0ea37ccc245d43f98a9484da4af44ba3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Mutation
86 rdf:type schema:DefinedTerm
87 N18600874616848f987f78666076d5422 schema:name dimensions_id
88 schema:value pub.1044720605
89 rdf:type schema:PropertyValue
90 N2dd9ee9747be428fb1b18d2ee1778730 schema:name Springer Nature - SN SciGraph project
91 rdf:type schema:Organization
92 N2e4f88d7d5234f988af3b83dc31f2793 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Mutagenesis
94 rdf:type schema:DefinedTerm
95 N2f6f3c24efb04bc2b470ebfe0cd979c8 schema:name pubmed_id
96 schema:value 16703468
97 rdf:type schema:PropertyValue
98 N31b31795d887466e998c0c6ea35752f8 schema:volumeNumber 25
99 rdf:type schema:PublicationVolume
100 N364ad02aa2e24d42a0abcee40307ea9a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Amino Acid Substitution
102 rdf:type schema:DefinedTerm
103 N6bfc776a043a429499f2cb8a8e9c8269 schema:issueNumber 4
104 rdf:type schema:PublicationIssue
105 N74279ee9d2d04694b3359d5ba5224b01 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Elapid Venoms
107 rdf:type schema:DefinedTerm
108 N7a8ef6bcfc9b4bc8828eacb9a5cff95d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Elapidae
110 rdf:type schema:DefinedTerm
111 N8f78c31dbcf247c2a8226a76273fe688 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Taiwan
113 rdf:type schema:DefinedTerm
114 N9a5ba94c645842f3a537c9170440ef24 rdf:first sg:person.0770353614.14
115 rdf:rest rdf:nil
116 Na68ae2be485546a9983eab98d3ba0e26 rdf:first sg:person.0625637355.97
117 rdf:rest N059282d4fcc34408afb734a1830fc602
118 Ne028b211c150422ea93dc03f87693c48 rdf:first sg:person.01234357506.31
119 rdf:rest Na68ae2be485546a9983eab98d3ba0e26
120 Ne44af95b36e84078ac10243dc0dd550f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Animals
122 rdf:type schema:DefinedTerm
123 Nf155d0b23d9a4d19919cc37cbeb35098 schema:name doi
124 schema:value 10.1007/s10930-006-9009-4
125 rdf:type schema:PropertyValue
126 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
127 schema:name Biological Sciences
128 rdf:type schema:DefinedTerm
129 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
130 schema:name Biochemistry and Cell Biology
131 rdf:type schema:DefinedTerm
132 sg:journal.1297375 schema:issn 1572-3887
133 1573-4943
134 schema:name The Protein Journal
135 schema:publisher Springer Nature
136 rdf:type schema:Periodical
137 sg:person.01234357506.31 schema:affiliation grid-institutes:grid.412036.2
138 schema:familyName Yan
139 schema:givenName Fang-Jiun
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234357506.31
141 rdf:type schema:Person
142 sg:person.0625637355.97 schema:affiliation grid-institutes:grid.412036.2
143 schema:familyName Chen
144 schema:givenName Ching-Ping
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625637355.97
146 rdf:type schema:Person
147 sg:person.0716147312.76 schema:affiliation grid-institutes:grid.412036.2
148 schema:familyName Cheng
149 schema:givenName Yun-Ching
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716147312.76
151 rdf:type schema:Person
152 sg:person.0770353614.14 schema:affiliation grid-institutes:grid.412036.2
153 schema:familyName Chang
154 schema:givenName Long-Sen
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770353614.14
156 rdf:type schema:Person
157 sg:pub.10.1007/bf01024879 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026609357
158 https://doi.org/10.1007/bf01024879
159 rdf:type schema:CreativeWork
160 grid-institutes:grid.412036.2 schema:alternateName Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan
161 schema:name Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan
162 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...