Ontology type: schema:ScholarlyArticle
2019-02
AUTHORSDavid Schweida, Pierre Barraud, Christof Regl, Fionna E. Loughlin, Christian G. Huber, Chiara Cabrele, Mario Schubert
ABSTRACTN-terminal gluconoylation is a moderately widespread modification in recombinant proteins expressed in Escherichia coli, in particular in proteins bearing an N-terminal histidine-tag. This post-translational modification has been investigated mainly by mass spectrometry. Although its NMR signals must have been observed earlier in spectra of 13C/15N labeled proteins, their chemical shifts were not yet reported. Here we present the complete 1H and 13C chemical shift assignment of the N-terminal gluconoyl post-translational modification, based on a selection of His-tagged protein constructs (CCL2, hnRNP A1 and Lin28) starting with Met-Gly-...-(His)6. In addition, we show that the modification can hydrolyze over time, resulting in a free N-terminus and gluconate. This leads to the disappearance of the gluconoyl signals and the appearance of gluconate signals during the NMR measurements. The chemical shifts presented here can now be used as a reference for the identification of gluconoylation in recombinant proteins, in particular when isotopically labeled. More... »
PAGES71-79
http://scigraph.springernature.com/pub.10.1007/s10858-019-00228-6
DOIhttp://dx.doi.org/10.1007/s10858-019-00228-6
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1112020493
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/30737614
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Salzburg",
"id": "https://www.grid.ac/institutes/grid.7039.d",
"name": [
"Department of Biosciences, University of Salzburg, Billrothstr. 11, 5020, Salzburg, Austria"
],
"type": "Organization"
},
"familyName": "Schweida",
"givenName": "David",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Swiss Federal Institute of Technology in Zurich",
"id": "https://www.grid.ac/institutes/grid.5801.c",
"name": [
"Institute of Molecular Biology and Biophysics, ETH Z\u00fcrich, 8093, Zurich, Switzerland",
"Institut de Biologie Physico-Chimique (IBPC), UMR 8261 CNRS, Universit\u00e9 Paris Diderot, 13 rue Pierre et Marie Curie, 75005, Paris, France"
],
"type": "Organization"
},
"familyName": "Barraud",
"givenName": "Pierre",
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Salzburg",
"id": "https://www.grid.ac/institutes/grid.7039.d",
"name": [
"Department of Biosciences, University of Salzburg, Billrothstr. 11, 5020, Salzburg, Austria",
"Christian Doppler Laboratory for Innovative Tools for Biosimilar Characterization, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria"
],
"type": "Organization"
},
"familyName": "Regl",
"givenName": "Christof",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Monash University",
"id": "https://www.grid.ac/institutes/grid.1002.3",
"name": [
"Institute of Molecular Biology and Biophysics, ETH Z\u00fcrich, 8093, Zurich, Switzerland",
"Department of Biochemistry & Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, 3800, Clayton, VIC, Australia"
],
"type": "Organization"
},
"familyName": "Loughlin",
"givenName": "Fionna E.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Salzburg",
"id": "https://www.grid.ac/institutes/grid.7039.d",
"name": [
"Department of Biosciences, University of Salzburg, Billrothstr. 11, 5020, Salzburg, Austria",
"Christian Doppler Laboratory for Innovative Tools for Biosimilar Characterization, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria"
],
"type": "Organization"
},
"familyName": "Huber",
"givenName": "Christian G.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Salzburg",
"id": "https://www.grid.ac/institutes/grid.7039.d",
"name": [
"Department of Biosciences, University of Salzburg, Billrothstr. 11, 5020, Salzburg, Austria",
"Christian Doppler Laboratory for Innovative Tools for Biosimilar Characterization, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria"
],
"type": "Organization"
},
"familyName": "Cabrele",
"givenName": "Chiara",
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Salzburg",
"id": "https://www.grid.ac/institutes/grid.7039.d",
"name": [
"Department of Biosciences, University of Salzburg, Billrothstr. 11, 5020, Salzburg, Austria",
"Institute of Molecular Biology and Biophysics, ETH Z\u00fcrich, 8093, Zurich, Switzerland",
"Christian Doppler Laboratory for Innovative Tools for Biosimilar Characterization, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria"
],
"type": "Organization"
},
"familyName": "Schubert",
"givenName": "Mario",
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1111/j.1432-1033.1986.tb09378.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001554564"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.pep.2005.04.018",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004189930"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.pep.2005.04.018",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004189930"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/anie.201502093",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004778258"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.ppat.1002706",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004976306"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nsmb.2202",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010994048",
"https://doi.org/10.1038/nsmb.2202"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/macp.1990.021911228",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015351775"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10858-012-9696-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015896176",
"https://doi.org/10.1007/s10858-012-9696-4"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1008290618449",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016396516",
"https://doi.org/10.1023/a:1008290618449"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/jmre.1998.1543",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017946994"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0022-2364(92)90144-v",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019780339"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.01790-07",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021073213"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/bbrc.1999.1304",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030594796"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/1099-0518(20001101)38:21<3892::aid-pola60>3.0.co;2-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035063742"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/cbic.201100654",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036759279"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/bbrc.1999.0770",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044412600"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0022-2364(90)90202-k",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045158398"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/abio.1998.2990",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047066676"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/mcp.m600225-mcp200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048410880"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.carres.2010.05.009",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050884965"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/ic500387u",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055568568"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/pr9011987",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056295300"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.7554/elife.25736",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1086257128"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/acs.analchem.7b01645",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092355464"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nchembio.2576",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101033134",
"https://doi.org/10.1038/nchembio.2576"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nchembio.2576",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101033134",
"https://doi.org/10.1038/nchembio.2576"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nchembio.2576",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101033134",
"https://doi.org/10.1038/nchembio.2576"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nchembio.2576",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101033134",
"https://doi.org/10.1038/nchembio.2576"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nchembio.2576",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101033134",
"https://doi.org/10.1038/nchembio.2576"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/acs.analchem.8b02637",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1106125173"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/acs.analchem.8b02637",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1106125173"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41467-018-05695-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1106133445",
"https://doi.org/10.1038/s41467-018-05695-3"
],
"type": "CreativeWork"
}
],
"datePublished": "2019-02",
"datePublishedReg": "2019-02-01",
"description": "N-terminal gluconoylation is a moderately widespread modification in recombinant proteins expressed in Escherichia coli, in particular in proteins bearing an N-terminal histidine-tag. This post-translational modification has been investigated mainly by mass spectrometry. Although its NMR signals must have been observed earlier in spectra of 13C/15N labeled proteins, their chemical shifts were not yet reported. Here we present the complete 1H and 13C chemical shift assignment of the N-terminal gluconoyl post-translational modification, based on a selection of His-tagged protein constructs (CCL2, hnRNP A1 and Lin28) starting with Met-Gly-...-(His)6. In addition, we show that the modification can hydrolyze over time, resulting in a free N-terminus and gluconate. This leads to the disappearance of the gluconoyl signals and the appearance of gluconate signals during the NMR measurements. The chemical shifts presented here can now be used as a reference for the identification of gluconoylation in recombinant proteins, in particular when isotopically labeled.",
"genre": "research_article",
"id": "sg:pub.10.1007/s10858-019-00228-6",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1101518",
"issn": [
"0925-2738",
"1573-5001"
],
"name": "Journal of Biomolecular NMR",
"type": "Periodical"
},
{
"issueNumber": "1-2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "73"
}
],
"name": "The NMR signature of gluconoylation: a frequent N-terminal modification of isotope-labeled proteins",
"pagination": "71-79",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"27e93f2cf968a7ff79a43c70fd2fb08494c2462d827aebddad8df71d79a98bd6"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"30737614"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"9110829"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s10858-019-00228-6"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1112020493"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s10858-019-00228-6",
"https://app.dimensions.ai/details/publication/pub.1112020493"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T13:33",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000370_0000000370/records_46765_00000003.jsonl",
"type": "ScholarlyArticle",
"url": "https://link.springer.com/10.1007%2Fs10858-019-00228-6"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10858-019-00228-6'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10858-019-00228-6'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10858-019-00228-6'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10858-019-00228-6'
This table displays all metadata directly associated to this object as RDF triples.
197 TRIPLES
21 PREDICATES
55 URIs
21 LITERALS
9 BLANK NODES