Spinning faster: protein NMR at MAS frequencies up to 126 kHz View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-02

AUTHORS

Susanne Penzel, Andres Oss, Mai-Liis Org, Ago Samoson, Anja Böckmann, Matthias Ernst, Beat H. Meier

ABSTRACT

We report linewidth and proton T1, T and T2' relaxation data of the model protein ubiquitin acquired at MAS frequencies up to 126 kHz. We find a predominantly linear improvement in linewidths and coherence decay times of protons with increasing spinning frequency in the range from 93 to 126 kHz. We further attempt to gain insight into the different contributions to the linewidth at fast MAS using site-specific analysis of proton relaxation parameters and present bulk relaxation times as a function of the MAS frequency. For microcrystalline fully-protonated ubiquitin, inhomogeneous contributions are only a minor part of the proton linewidth, and at 126 kHz MAS coherent effects are still dominating. We furthermore present site-specific proton relaxation rate constants during a spinlock at 126 kHz MAS, as well as MAS-dependent bulk T (1HN). More... »

PAGES

19-29

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10858-018-0219-9

DOI

http://dx.doi.org/10.1007/s10858-018-0219-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111642845

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30680507


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0299", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Other Physical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/02", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Physical Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Physical Chemistry, ETH Z\u00fcrich, Vladimir-Prelog-Weg 2, 8093, Zurich, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Penzel", 
        "givenName": "Susanne", 
        "id": "sg:person.01261261666.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261261666.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tallinn University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6988.f", 
          "name": [
            "NMR Instituut, Tartu Teaduspark, Tehnomeedikum, Tallinn University of Technology, Akadeemia tee 15a, 19086, Tallinn, Estonia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Oss", 
        "givenName": "Andres", 
        "id": "sg:person.012650257555.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012650257555.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tallinn University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6988.f", 
          "name": [
            "NMR Instituut, Tartu Teaduspark, Tehnomeedikum, Tallinn University of Technology, Akadeemia tee 15a, 19086, Tallinn, Estonia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Org", 
        "givenName": "Mai-Liis", 
        "id": "sg:person.01135624741.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135624741.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tallinn University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6988.f", 
          "name": [
            "NMR Instituut, Tartu Teaduspark, Tehnomeedikum, Tallinn University of Technology, Akadeemia tee 15a, 19086, Tallinn, Estonia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Samoson", 
        "givenName": "Ago", 
        "id": "sg:person.0647345040.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647345040.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Lyon System", 
          "id": "https://www.grid.ac/institutes/grid.25697.3f", 
          "name": [
            "Institut de Biologie et Chimie des Prot\u00e9ines, UMR 5086 CNRS/Universit\u00e9 de Lyon 1, Labex ECOFECT, 7, Passage du Vercors, 69367, Lyon, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "B\u00f6ckmann", 
        "givenName": "Anja", 
        "id": "sg:person.01202540744.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202540744.82"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Physical Chemistry, ETH Z\u00fcrich, Vladimir-Prelog-Weg 2, 8093, Zurich, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ernst", 
        "givenName": "Matthias", 
        "id": "sg:person.01075267274.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01075267274.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Physical Chemistry, ETH Z\u00fcrich, Vladimir-Prelog-Weg 2, 8093, Zurich, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Meier", 
        "givenName": "Beat H.", 
        "id": "sg:person.0621622733.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0621622733.90"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0022-2364(83)90207-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002617028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmr.2008.01.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003439574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201607084", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004807272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1090-7807(02)00035-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005109039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1090-7807(02)00035-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005109039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmr.2015.01.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008633378"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja100726a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009850348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja100726a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009850348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1214/aos/1176344552", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012894299"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cplett.2006.06.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012975024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja981546c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013308082"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja507382j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013579110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1110/ps.041139505", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013709679"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-006-9025-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020974107", 
          "https://doi.org/10.1007/s10858-006-9025-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cplett.2008.11.089", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026296602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cphc.200900439", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030159823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cphc.200900439", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030159823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1602248113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030217043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1602248113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030217043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmr.2007.05.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032193364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-009-9374-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033669162", 
          "https://doi.org/10.1007/s10858-009-9374-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-009-9374-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033669162", 
          "https://doi.org/10.1007/s10858-009-9374-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-005-0658-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033766190", 
          "https://doi.org/10.1007/s10858-005-0658-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-005-0658-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033766190", 
          "https://doi.org/10.1007/s10858-005-0658-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-005-0658-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033766190", 
          "https://doi.org/10.1007/s10858-005-0658-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.200600328", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037213867"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmr.2008.04.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039748957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201405730", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040442026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmr.2013.12.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043412057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-016-0040-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046051581", 
          "https://doi.org/10.1007/s10858-016-0040-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cphc.201100062", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046918336"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-016-0047-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049470768", 
          "https://doi.org/10.1007/s10858-016-0047-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-016-0047-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049470768", 
          "https://doi.org/10.1007/s10858-016-0047-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2014.05.048", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050354352"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201008244", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050602943"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-015-9975-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051140492", 
          "https://doi.org/10.1007/s10858-015-9975-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmr.2014.09.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052630360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/1821659a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053527752", 
          "https://doi.org/10.1038/1821659a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0027231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055168077"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0027231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055168077"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja030546w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055832519"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja030546w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055832519"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja0369966", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055834171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja0369966", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055834171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja0494510", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055838087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja0494510", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055838087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja064379m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055842722"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja064379m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055842722"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja110222h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055849029"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja110222h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055849029"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.3658383", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057993481"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.437915", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058015944"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrev.175.453", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060439883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrev.175.453", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060439883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrev.185.420", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060442653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrev.185.420", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060442653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrev.80.580", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060457052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrev.80.580", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060457052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.2.285", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060770662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.2.285", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060770662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-016-0085-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083535075", 
          "https://doi.org/10.1007/s10858-016-0085-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-016-0085-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083535075", 
          "https://doi.org/10.1007/s10858-016-0085-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ssnmr.2017.04.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084845256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/chem.201701738", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085213382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acs.jpcb.7b03333", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085616265"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201712091", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101105531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201712091", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101105531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmr.2018.04.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103181239"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmr.2018.06.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104601801"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acs.jpcc.8b05600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104897839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c8cc05882b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1106393940"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-02", 
    "datePublishedReg": "2019-02-01", 
    "description": "We report linewidth and proton T1, T1\u03c1 and T2' relaxation data of the model protein ubiquitin acquired at MAS frequencies up to 126\u00a0kHz. We find a predominantly linear improvement in linewidths and coherence decay times of protons with increasing spinning frequency in the range from 93 to 126\u00a0kHz. We further attempt to gain insight into the different contributions to the linewidth at fast MAS using site-specific analysis of proton relaxation parameters and present bulk relaxation times as a function of the MAS frequency. For microcrystalline fully-protonated ubiquitin, inhomogeneous contributions are only a minor part of the proton linewidth, and at 126\u00a0kHz MAS coherent effects are still dominating. We furthermore present site-specific proton relaxation rate constants during a spinlock at 126\u00a0kHz MAS, as well as MAS-dependent bulk T1\u03c1 (1HN).", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10858-018-0219-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5180950", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6814619", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6576183", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1101518", 
        "issn": [
          "0925-2738", 
          "1573-5001"
        ], 
        "name": "Journal of Biomolecular NMR", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1-2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "73"
      }
    ], 
    "name": "Spinning faster: protein NMR at MAS frequencies up to 126 kHz", 
    "pagination": "19-29", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "67e1f148694c7bd1c3409da7e2f69e3ac9dee5db246da10c9e1dab7f11f280ca"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30680507"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9110829"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10858-018-0219-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1111642845"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10858-018-0219-9", 
      "https://app.dimensions.ai/details/publication/pub.1111642845"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000370_0000000370/records_46737_00000003.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs10858-018-0219-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10858-018-0219-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10858-018-0219-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10858-018-0219-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10858-018-0219-9'


 

This table displays all metadata directly associated to this object as RDF triples.

284 TRIPLES      21 PREDICATES      80 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10858-018-0219-9 schema:about anzsrc-for:02
2 anzsrc-for:0299
3 schema:author N188656efbaf8490182e2a1434332cfdf
4 schema:citation sg:pub.10.1007/s10858-005-0658-y
5 sg:pub.10.1007/s10858-006-9025-x
6 sg:pub.10.1007/s10858-009-9374-3
7 sg:pub.10.1007/s10858-015-9975-y
8 sg:pub.10.1007/s10858-016-0040-2
9 sg:pub.10.1007/s10858-016-0047-8
10 sg:pub.10.1007/s10858-016-0085-2
11 sg:pub.10.1038/1821659a0
12 https://doi.org/10.1002/anie.200600328
13 https://doi.org/10.1002/anie.201008244
14 https://doi.org/10.1002/anie.201405730
15 https://doi.org/10.1002/anie.201607084
16 https://doi.org/10.1002/anie.201712091
17 https://doi.org/10.1002/chem.201701738
18 https://doi.org/10.1002/cphc.200900439
19 https://doi.org/10.1002/cphc.201100062
20 https://doi.org/10.1016/0022-2364(83)90207-x
21 https://doi.org/10.1016/j.bpj.2014.05.048
22 https://doi.org/10.1016/j.cplett.2006.06.007
23 https://doi.org/10.1016/j.cplett.2008.11.089
24 https://doi.org/10.1016/j.jmr.2007.05.005
25 https://doi.org/10.1016/j.jmr.2008.01.012
26 https://doi.org/10.1016/j.jmr.2008.04.021
27 https://doi.org/10.1016/j.jmr.2013.12.010
28 https://doi.org/10.1016/j.jmr.2014.09.007
29 https://doi.org/10.1016/j.jmr.2015.01.012
30 https://doi.org/10.1016/j.jmr.2018.04.003
31 https://doi.org/10.1016/j.jmr.2018.06.002
32 https://doi.org/10.1016/j.ssnmr.2017.04.002
33 https://doi.org/10.1016/s1090-7807(02)00035-6
34 https://doi.org/10.1021/acs.jpcb.7b03333
35 https://doi.org/10.1021/acs.jpcc.8b05600
36 https://doi.org/10.1021/bi0027231
37 https://doi.org/10.1021/ja030546w
38 https://doi.org/10.1021/ja0369966
39 https://doi.org/10.1021/ja0494510
40 https://doi.org/10.1021/ja064379m
41 https://doi.org/10.1021/ja100726a
42 https://doi.org/10.1021/ja110222h
43 https://doi.org/10.1021/ja507382j
44 https://doi.org/10.1021/ja981546c
45 https://doi.org/10.1039/c8cc05882b
46 https://doi.org/10.1063/1.3658383
47 https://doi.org/10.1063/1.437915
48 https://doi.org/10.1073/pnas.1602248113
49 https://doi.org/10.1103/physrev.175.453
50 https://doi.org/10.1103/physrev.185.420
51 https://doi.org/10.1103/physrev.80.580
52 https://doi.org/10.1103/physrevlett.2.285
53 https://doi.org/10.1110/ps.041139505
54 https://doi.org/10.1214/aos/1176344552
55 schema:datePublished 2019-02
56 schema:datePublishedReg 2019-02-01
57 schema:description We report linewidth and proton T<sub>1</sub>, T<sub>1ρ</sub> and T<sub>2</sub>' relaxation data of the model protein ubiquitin acquired at MAS frequencies up to 126 kHz. We find a predominantly linear improvement in linewidths and coherence decay times of protons with increasing spinning frequency in the range from 93 to 126 kHz. We further attempt to gain insight into the different contributions to the linewidth at fast MAS using site-specific analysis of proton relaxation parameters and present bulk relaxation times as a function of the MAS frequency. For microcrystalline fully-protonated ubiquitin, inhomogeneous contributions are only a minor part of the proton linewidth, and at 126 kHz MAS coherent effects are still dominating. We furthermore present site-specific proton relaxation rate constants during a spinlock at 126 kHz MAS, as well as MAS-dependent bulk T<sub>1ρ</sub> (<sup>1</sup>H<sup>N</sup>).
58 schema:genre research_article
59 schema:inLanguage en
60 schema:isAccessibleForFree false
61 schema:isPartOf N7e12e11cf61a442da2c2bddc2b8be3dd
62 N967ae28a74094c3fa7618c44625013c5
63 sg:journal.1101518
64 schema:name Spinning faster: protein NMR at MAS frequencies up to 126 kHz
65 schema:pagination 19-29
66 schema:productId N138dae7e0360437cb595fa364e770ea2
67 Nb4379b00127e447f9ef088c383b4f968
68 Nc1e248e0ac1d4d55ae14dc4c10b747ea
69 Nda20657c611f4606992fc1a1ac6eedca
70 Nf917fef8ede0405db195d897464417fd
71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111642845
72 https://doi.org/10.1007/s10858-018-0219-9
73 schema:sdDatePublished 2019-04-11T13:27
74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
75 schema:sdPublisher N25062d9de6574f9b94e84eb91f18e81e
76 schema:url https://link.springer.com/10.1007%2Fs10858-018-0219-9
77 sgo:license sg:explorer/license/
78 sgo:sdDataset articles
79 rdf:type schema:ScholarlyArticle
80 N119fd0be3df14bf2aac68b966ae696ca rdf:first sg:person.0647345040.05
81 rdf:rest Nc438e800e9d44ef79294cae1d9530529
82 N138dae7e0360437cb595fa364e770ea2 schema:name readcube_id
83 schema:value 67e1f148694c7bd1c3409da7e2f69e3ac9dee5db246da10c9e1dab7f11f280ca
84 rdf:type schema:PropertyValue
85 N154f3d9aa59a4d86a12358b6480535b6 rdf:first sg:person.0621622733.90
86 rdf:rest rdf:nil
87 N188656efbaf8490182e2a1434332cfdf rdf:first sg:person.01261261666.14
88 rdf:rest N3fdab10d98fe4171844e12ce251a621e
89 N25062d9de6574f9b94e84eb91f18e81e schema:name Springer Nature - SN SciGraph project
90 rdf:type schema:Organization
91 N392afd99314246c2bba5074848507b37 rdf:first sg:person.01075267274.97
92 rdf:rest N154f3d9aa59a4d86a12358b6480535b6
93 N3fdab10d98fe4171844e12ce251a621e rdf:first sg:person.012650257555.01
94 rdf:rest Ne3f45e18ef124a8dae2ee00e93034d5c
95 N7e12e11cf61a442da2c2bddc2b8be3dd schema:volumeNumber 73
96 rdf:type schema:PublicationVolume
97 N967ae28a74094c3fa7618c44625013c5 schema:issueNumber 1-2
98 rdf:type schema:PublicationIssue
99 Nb4379b00127e447f9ef088c383b4f968 schema:name pubmed_id
100 schema:value 30680507
101 rdf:type schema:PropertyValue
102 Nc1e248e0ac1d4d55ae14dc4c10b747ea schema:name nlm_unique_id
103 schema:value 9110829
104 rdf:type schema:PropertyValue
105 Nc438e800e9d44ef79294cae1d9530529 rdf:first sg:person.01202540744.82
106 rdf:rest N392afd99314246c2bba5074848507b37
107 Nda20657c611f4606992fc1a1ac6eedca schema:name doi
108 schema:value 10.1007/s10858-018-0219-9
109 rdf:type schema:PropertyValue
110 Ne3f45e18ef124a8dae2ee00e93034d5c rdf:first sg:person.01135624741.52
111 rdf:rest N119fd0be3df14bf2aac68b966ae696ca
112 Nf917fef8ede0405db195d897464417fd schema:name dimensions_id
113 schema:value pub.1111642845
114 rdf:type schema:PropertyValue
115 anzsrc-for:02 schema:inDefinedTermSet anzsrc-for:
116 schema:name Physical Sciences
117 rdf:type schema:DefinedTerm
118 anzsrc-for:0299 schema:inDefinedTermSet anzsrc-for:
119 schema:name Other Physical Sciences
120 rdf:type schema:DefinedTerm
121 sg:grant.5180950 http://pending.schema.org/fundedItem sg:pub.10.1007/s10858-018-0219-9
122 rdf:type schema:MonetaryGrant
123 sg:grant.6576183 http://pending.schema.org/fundedItem sg:pub.10.1007/s10858-018-0219-9
124 rdf:type schema:MonetaryGrant
125 sg:grant.6814619 http://pending.schema.org/fundedItem sg:pub.10.1007/s10858-018-0219-9
126 rdf:type schema:MonetaryGrant
127 sg:journal.1101518 schema:issn 0925-2738
128 1573-5001
129 schema:name Journal of Biomolecular NMR
130 rdf:type schema:Periodical
131 sg:person.01075267274.97 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
132 schema:familyName Ernst
133 schema:givenName Matthias
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01075267274.97
135 rdf:type schema:Person
136 sg:person.01135624741.52 schema:affiliation https://www.grid.ac/institutes/grid.6988.f
137 schema:familyName Org
138 schema:givenName Mai-Liis
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135624741.52
140 rdf:type schema:Person
141 sg:person.01202540744.82 schema:affiliation https://www.grid.ac/institutes/grid.25697.3f
142 schema:familyName Böckmann
143 schema:givenName Anja
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202540744.82
145 rdf:type schema:Person
146 sg:person.01261261666.14 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
147 schema:familyName Penzel
148 schema:givenName Susanne
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261261666.14
150 rdf:type schema:Person
151 sg:person.012650257555.01 schema:affiliation https://www.grid.ac/institutes/grid.6988.f
152 schema:familyName Oss
153 schema:givenName Andres
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012650257555.01
155 rdf:type schema:Person
156 sg:person.0621622733.90 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
157 schema:familyName Meier
158 schema:givenName Beat H.
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0621622733.90
160 rdf:type schema:Person
161 sg:person.0647345040.05 schema:affiliation https://www.grid.ac/institutes/grid.6988.f
162 schema:familyName Samoson
163 schema:givenName Ago
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647345040.05
165 rdf:type schema:Person
166 sg:pub.10.1007/s10858-005-0658-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1033766190
167 https://doi.org/10.1007/s10858-005-0658-y
168 rdf:type schema:CreativeWork
169 sg:pub.10.1007/s10858-006-9025-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020974107
170 https://doi.org/10.1007/s10858-006-9025-x
171 rdf:type schema:CreativeWork
172 sg:pub.10.1007/s10858-009-9374-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033669162
173 https://doi.org/10.1007/s10858-009-9374-3
174 rdf:type schema:CreativeWork
175 sg:pub.10.1007/s10858-015-9975-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1051140492
176 https://doi.org/10.1007/s10858-015-9975-y
177 rdf:type schema:CreativeWork
178 sg:pub.10.1007/s10858-016-0040-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046051581
179 https://doi.org/10.1007/s10858-016-0040-2
180 rdf:type schema:CreativeWork
181 sg:pub.10.1007/s10858-016-0047-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049470768
182 https://doi.org/10.1007/s10858-016-0047-8
183 rdf:type schema:CreativeWork
184 sg:pub.10.1007/s10858-016-0085-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083535075
185 https://doi.org/10.1007/s10858-016-0085-2
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/1821659a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053527752
188 https://doi.org/10.1038/1821659a0
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1002/anie.200600328 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037213867
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1002/anie.201008244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050602943
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1002/anie.201405730 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040442026
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1002/anie.201607084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004807272
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1002/anie.201712091 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101105531
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1002/chem.201701738 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085213382
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1002/cphc.200900439 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030159823
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1002/cphc.201100062 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046918336
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/0022-2364(83)90207-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002617028
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.bpj.2014.05.048 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050354352
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/j.cplett.2006.06.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012975024
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/j.cplett.2008.11.089 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026296602
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/j.jmr.2007.05.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032193364
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/j.jmr.2008.01.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003439574
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/j.jmr.2008.04.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039748957
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/j.jmr.2013.12.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043412057
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/j.jmr.2014.09.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052630360
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/j.jmr.2015.01.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008633378
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/j.jmr.2018.04.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103181239
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.jmr.2018.06.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104601801
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/j.ssnmr.2017.04.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084845256
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/s1090-7807(02)00035-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005109039
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1021/acs.jpcb.7b03333 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085616265
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1021/acs.jpcc.8b05600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104897839
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1021/bi0027231 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055168077
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1021/ja030546w schema:sameAs https://app.dimensions.ai/details/publication/pub.1055832519
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1021/ja0369966 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055834171
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1021/ja0494510 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055838087
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1021/ja064379m schema:sameAs https://app.dimensions.ai/details/publication/pub.1055842722
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1021/ja100726a schema:sameAs https://app.dimensions.ai/details/publication/pub.1009850348
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1021/ja110222h schema:sameAs https://app.dimensions.ai/details/publication/pub.1055849029
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1021/ja507382j schema:sameAs https://app.dimensions.ai/details/publication/pub.1013579110
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1021/ja981546c schema:sameAs https://app.dimensions.ai/details/publication/pub.1013308082
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1039/c8cc05882b schema:sameAs https://app.dimensions.ai/details/publication/pub.1106393940
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1063/1.3658383 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057993481
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1063/1.437915 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058015944
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1073/pnas.1602248113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030217043
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1103/physrev.175.453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060439883
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1103/physrev.185.420 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060442653
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1103/physrev.80.580 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060457052
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1103/physrevlett.2.285 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060770662
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1110/ps.041139505 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013709679
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1214/aos/1176344552 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012894299
275 rdf:type schema:CreativeWork
276 https://www.grid.ac/institutes/grid.25697.3f schema:alternateName University of Lyon System
277 schema:name Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université de Lyon 1, Labex ECOFECT, 7, Passage du Vercors, 69367, Lyon, France
278 rdf:type schema:Organization
279 https://www.grid.ac/institutes/grid.5801.c schema:alternateName Swiss Federal Institute of Technology in Zurich
280 schema:name Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2, 8093, Zurich, Switzerland
281 rdf:type schema:Organization
282 https://www.grid.ac/institutes/grid.6988.f schema:alternateName Tallinn University of Technology
283 schema:name NMR Instituut, Tartu Teaduspark, Tehnomeedikum, Tallinn University of Technology, Akadeemia tee 15a, 19086, Tallinn, Estonia
284 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...