Development and application of aromatic [13C, 1H] SOFAST-HMQC NMR experiment for nucleic acids View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-11

AUTHORS

Bharathwaj Sathyamoorthy, Janghyun Lee, Isaac Kimsey, Laura R. Ganser, Hashim Al-Hashimi

ABSTRACT

Higher sensitivity of NMR spectrometers and novel isotopic labeling schemes have ushered the development of rapid data acquisition methodologies, improving the time resolution with which NMR data can be acquired. For nucleic acids, longitudinal relaxation optimization in conjunction with Ernst angle excitation (SOFAST-HMQC) for imino protons, in addition to rendering rapid pulsing, has been demonstrated to yield significant improvements in sensitivity per unit time. Extending such methodology to other spins offers a viable prospect to measure additional chemical shifts, thereby broadening their utilization for various applications. Here, we introduce the 2D [(13)C, (1)H] aromatic SOFAST-HMQC that results in overall sensitivity gain of 1.4- to 1.7-fold relative to the conventional HMQC and can also be extended to yield long-range heteronuclear chemical shifts such as the adenine imino nitrogens N1, N3, N7 and N9. The applications of these experiments range from monitoring real-time biochemical processes, drug/ligand screening, and to collecting data at very low sample concentration and/or in cases where isotopic enrichment cannot be achieved. More... »

PAGES

77-83

References to SciGraph publications

  • 2011-09. Measurement of 1H–15N and 1H–13C residual dipolar couplings in nucleic acids from TROSY intensities in JOURNAL OF BIOMOLECULAR NMR
  • 1997-12. Fast-HMQC using Ernst angle pulses: An efficient tool for screening of ligand binding to target proteins in JOURNAL OF BIOMOLECULAR NMR
  • 2001-09. Prediction of proton chemical shifts in RNA – Their use in structure refinement and validation in JOURNAL OF BIOMOLECULAR NMR
  • 2012-02. Sugar-to-base correlation in nucleic acids with a 5D APSY-HCNCH or two 3D APSY-HCN experiments in JOURNAL OF BIOMOLECULAR NMR
  • 2012-02. Functional complexity and regulation through RNA dynamics in NATURE
  • 2013-07. Three-state mechanism couples ligand and temperature sensing in riboswitches in NATURE
  • 2005-06. Resolution enhanced homonuclear carbon decoupled triple resonance experiments for unambiguous RNA structural characterization in JOURNAL OF BIOMOLECULAR NMR
  • 2011-08. Discovery of selective bioactive small molecules by targeting an RNA dynamic ensemble in NATURE CHEMICAL BIOLOGY
  • 2012-12. GFT projection NMR for efficient 1H/13C sugar spin system identification in nucleic acids in JOURNAL OF BIOMOLECULAR NMR
  • 2005-12. SOFAST-HMQC Experiments for Recording Two-dimensional Deteronuclear Correlation Spectra of Proteins within a Few Seconds in JOURNAL OF BIOMOLECULAR NMR
  • 2013-01. Database proton NMR chemical shifts for RNA signal assignment and validation in JOURNAL OF BIOMOLECULAR NMR
  • 2001-12. Automated prediction of 15N, 13Cα, 13Cβ and 13C′ chemical shifts in proteins using a density functional database in JOURNAL OF BIOMOLECULAR NMR
  • 2014-04. Structure determination of noncanonical RNA motifs guided by 1H NMR chemical shifts in NATURE METHODS
  • 1997-12. Analysis of 1H chemical shifts in DNA: Assessment of the reliability of 1H chemical shift calculations for use in structure refinement in JOURNAL OF BIOMOLECULAR NMR
  • 2013-06. Nucleic acid helix structure determination from NMR proton chemical shifts in JOURNAL OF BIOMOLECULAR NMR
  • 1992-11. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions in JOURNAL OF BIOMOLECULAR NMR
  • 2014-02. Time-resolved multidimensional NMR with non-uniform sampling in JOURNAL OF BIOMOLECULAR NMR
  • 2012-04. Application of iterative soft thresholding for fast reconstruction of NMR data non-uniformly sampled with multidimensional Poisson Gap scheduling in JOURNAL OF BIOMOLECULAR NMR
  • 1994-01. Correlation of nucleotide base and sugar protons in a 15N-labeled HIV-1 RNA oligonucleotide by 1H-15N HSQC experiments in JOURNAL OF BIOMOLECULAR NMR
  • 1995-11. NMRPipe: A multidimensional spectral processing system based on UNIX pipes in JOURNAL OF BIOMOLECULAR NMR
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10858-014-9856-9

    DOI

    http://dx.doi.org/10.1007/s10858-014-9856-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1047107785

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25186910


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