Approaches to the assignment of 19F resonances from 3-fluorophenylalanine labeled calmodulin using solution state NMR View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-06

AUTHORS

Julianne L. Kitevski-LeBlanc, Ferenc Evanics, R. Scott Prosser

ABSTRACT

Traditional single site replacement mutations (in this case, phenylalanine to tyrosine) were compared with methods which exclusively employ (15)N and (19)F-edited two- and three-dimensional NMR experiments for purposes of assigning (19)F NMR resonances from calmodulin (CaM), biosynthetically labeled with 3-fluorophenylalanine (3-FPhe). The global substitution of 3-FPhe for native phenylalanine was tolerated in CaM as evidenced by a comparison of (1)H-(15)N HSQC spectra and calcium binding assays in the presence and absence of 3-FPhe. The (19)F NMR spectrum reveals six resolved resonances, one of which integrates to three 3-FPhe species, making for a total of eight fluorophenylalanines. Single phenylalanine to tyrosine mutants of five phenylalanine positions resulted in (19)F NMR spectra with significant chemical shift perturbations of the remaining resonances, and provided only a single definitive assignment. Although (1)H-(19)F heteronucleclear NOEs proved weak, (19)F-edited (1)H-(1)H NOESY connectivities were relatively easy to establish by making use of the (3)J(FH) coupling between the fluorine nucleus and the adjacent fluorophenylalanine delta proton. (19)F-edited NOESY connectivities between the delta protons and alpha and beta nuclei in addition to (15)N-edited (1)H, (1)H NOESY crosspeaks proved sufficient to assign 4 of 8 (19)F resonances. Controlled cleavage of the protein into two fragments using trypsin, and a repetition of the above 2D and 3D techniques resulted in unambiguous assignments of all 8 (19)F NMR resonances. Our studies suggest that (19)F-edited NOESY NMR spectra are generally adequate for complete assignment without the need to resort to mutational analysis. More... »

PAGES

113-123

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10858-010-9415-y

DOI

http://dx.doi.org/10.1007/s10858-010-9415-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038016714

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20401735


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calmodulin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Crystallography, X-Ray", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis, Site-Directed", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nitrogen Isotopes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Magnetic Resonance, Biomolecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptide Fragments", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenylalanine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Solutions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trypsin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tyrosine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Xenopus Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Toronto", 
          "id": "https://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Department of Chemistry, University of Toronto, UTM, 3359 Mississauga Rd. North, L5L 1C6, Mississauga, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kitevski-LeBlanc", 
        "givenName": "Julianne L.", 
        "id": "sg:person.01220465703.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220465703.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Toronto", 
          "id": "https://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Department of Chemistry, University of Toronto, UTM, 3359 Mississauga Rd. North, L5L 1C6, Mississauga, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Evanics", 
        "givenName": "Ferenc", 
        "id": "sg:person.01361657751.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361657751.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Toronto", 
          "id": "https://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Department of Chemistry, University of Toronto, UTM, 3359 Mississauga Rd. North, L5L 1C6, Mississauga, ON, Canada", 
            "Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Scott Prosser", 
        "givenName": "R.", 
        "id": "sg:person.01171427402.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171427402.14"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1006/jmbi.1998.1935", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000010976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abio.2002.5661", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000806680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00197809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001469691", 
          "https://doi.org/10.1007/bf00197809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00197809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001469691", 
          "https://doi.org/10.1007/bf00197809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2744(77)90199-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001576498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2744(77)90199-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001576498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1999.3188", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001931600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00404272", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004332552", 
          "https://doi.org/10.1007/bf00404272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00404272", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004332552", 
          "https://doi.org/10.1007/bf00404272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0705253104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005534760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(02)00682-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007001088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.19.11284", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011769863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbagen.2006.10.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012292357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0969-2126(00)00544-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014373103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bb.24.060195.000505", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015489796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(90)90190-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018188671"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(90)90190-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018188671"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bb.25.060196.001115", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020433849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1097-458x(200007)38:7<551::aid-mrc686>3.0.co;2-q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020957930"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cmr.10035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026082447"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb0995-758", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026576907", 
          "https://doi.org/10.1038/nsb0995-758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bb.06.060177.001055", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027823832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10858-004-3534-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028093136", 
          "https://doi.org/10.1007/s10858-004-3534-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/snmr.2002.0063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029960993"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/snmr.2002.0063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029960993"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb0995-777", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033189599", 
          "https://doi.org/10.1038/nsb0995-777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2004.12.058", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034801867"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0959-440x(00)00234-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037648308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(02)00465-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041550713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00185455", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041846678", 
          "https://doi.org/10.1007/bf00185455"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.273.41.26375", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046767462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.str.2003.09.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048409736"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.str.2003.09.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048409736"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0079-6565(94)80009-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051298558"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0308641101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051783879"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi000582x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055159139"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi000582x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055159139"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00138a005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055162267"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9628275", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055213215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9628275", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055213215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00076a099", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055704798"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00087a058", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055705760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00353a071", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055725893"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja0041876", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055730900"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja0041876", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055730900"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja060618u", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055841592"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja060618u", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055841592"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja8085752", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055860044"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja8085752", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055860044"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja960465i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055864741"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja960465i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055864741"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077812513", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077991809", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082839909", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2010-06", 
    "datePublishedReg": "2010-06-01", 
    "description": "Traditional single site replacement mutations (in this case, phenylalanine to tyrosine) were compared with methods which exclusively employ (15)N and (19)F-edited two- and three-dimensional NMR experiments for purposes of assigning (19)F NMR resonances from calmodulin (CaM), biosynthetically labeled with 3-fluorophenylalanine (3-FPhe). The global substitution of 3-FPhe for native phenylalanine was tolerated in CaM as evidenced by a comparison of (1)H-(15)N HSQC spectra and calcium binding assays in the presence and absence of 3-FPhe. The (19)F NMR spectrum reveals six resolved resonances, one of which integrates to three 3-FPhe species, making for a total of eight fluorophenylalanines. Single phenylalanine to tyrosine mutants of five phenylalanine positions resulted in (19)F NMR spectra with significant chemical shift perturbations of the remaining resonances, and provided only a single definitive assignment. Although (1)H-(19)F heteronucleclear NOEs proved weak, (19)F-edited (1)H-(1)H NOESY connectivities were relatively easy to establish by making use of the (3)J(FH) coupling between the fluorine nucleus and the adjacent fluorophenylalanine delta proton. (19)F-edited NOESY connectivities between the delta protons and alpha and beta nuclei in addition to (15)N-edited (1)H, (1)H NOESY crosspeaks proved sufficient to assign 4 of 8 (19)F resonances. Controlled cleavage of the protein into two fragments using trypsin, and a repetition of the above 2D and 3D techniques resulted in unambiguous assignments of all 8 (19)F NMR resonances. Our studies suggest that (19)F-edited NOESY NMR spectra are generally adequate for complete assignment without the need to resort to mutational analysis.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10858-010-9415-y", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1101518", 
        "issn": [
          "0925-2738", 
          "1573-5001"
        ], 
        "name": "Journal of Biomolecular NMR", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "47"
      }
    ], 
    "name": "Approaches to the assignment of 19F resonances from 3-fluorophenylalanine labeled calmodulin using solution state NMR", 
    "pagination": "113-123", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b519814ab60a65e7b1c6d08cd97103d3a593a8206f03c4590e43adf30266de1f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "20401735"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9110829"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10858-010-9415-y"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1038016714"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10858-010-9415-y", 
      "https://app.dimensions.ai/details/publication/pub.1038016714"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:17", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000348_0000000348/records_54313_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s10858-010-9415-y"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10858-010-9415-y'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10858-010-9415-y'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10858-010-9415-y'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10858-010-9415-y'


 

This table displays all metadata directly associated to this object as RDF triples.

277 TRIPLES      21 PREDICATES      87 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10858-010-9415-y schema:about N0fd1536335d3499bb1c0963e221242e0
2 N104586b0ccb74502bf86ee4fdd63df24
3 N120cbb6458a049bb8a8a4c21d2a86ce2
4 N19efa5c2226245fea77639002698652b
5 N3e5afb03807c477d9e39a9f95ce7e5aa
6 N42bd22dfbd6b4150b5f7d88063055c14
7 N4394d6890bd845eca977cdba32b109a3
8 N4768efaef1fc42619d74f40b21e3222f
9 N4b6946f2e6694a4ab54669d8a59427b2
10 N52bcd408162e463cb6045234735e7177
11 N6290a67d47394e84ba92a4a5cead004e
12 N62dd3a2091e54b12be52eeb21d15b5ba
13 N89d4586739b7407a8b2499522cc2fbee
14 N98068e87011d4bcd874418e7c1ba30b9
15 Nb56e5cacdd1040878f48c38efbc54328
16 Nb704fb660cad4558ba5d7769048075dd
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author Nfb8409fdf59c4960b53564a5c1d5910f
20 schema:citation sg:pub.10.1007/bf00185455
21 sg:pub.10.1007/bf00197809
22 sg:pub.10.1007/bf00404272
23 sg:pub.10.1007/s10858-004-3534-2
24 sg:pub.10.1038/nsb0995-758
25 sg:pub.10.1038/nsb0995-777
26 https://app.dimensions.ai/details/publication/pub.1077812513
27 https://app.dimensions.ai/details/publication/pub.1077991809
28 https://app.dimensions.ai/details/publication/pub.1082839909
29 https://doi.org/10.1002/1097-458x(200007)38:7<551::aid-mrc686>3.0.co;2-q
30 https://doi.org/10.1002/cmr.10035
31 https://doi.org/10.1006/abio.2002.5661
32 https://doi.org/10.1006/jmbi.1998.1935
33 https://doi.org/10.1006/jmbi.1999.3188
34 https://doi.org/10.1006/snmr.2002.0063
35 https://doi.org/10.1016/0005-2744(77)90199-1
36 https://doi.org/10.1016/0006-2952(90)90190-v
37 https://doi.org/10.1016/0079-6565(94)80009-x
38 https://doi.org/10.1016/j.bbagen.2006.10.017
39 https://doi.org/10.1016/j.jmb.2004.12.058
40 https://doi.org/10.1016/j.str.2003.09.004
41 https://doi.org/10.1016/s0022-2836(02)00465-5
42 https://doi.org/10.1016/s0092-8674(02)00682-7
43 https://doi.org/10.1016/s0959-440x(00)00234-7
44 https://doi.org/10.1016/s0969-2126(00)00544-x
45 https://doi.org/10.1021/bi000582x
46 https://doi.org/10.1021/bi00138a005
47 https://doi.org/10.1021/bi9628275
48 https://doi.org/10.1021/ja00076a099
49 https://doi.org/10.1021/ja00087a058
50 https://doi.org/10.1021/ja00353a071
51 https://doi.org/10.1021/ja0041876
52 https://doi.org/10.1021/ja060618u
53 https://doi.org/10.1021/ja8085752
54 https://doi.org/10.1021/ja960465i
55 https://doi.org/10.1073/pnas.0308641101
56 https://doi.org/10.1073/pnas.0705253104
57 https://doi.org/10.1074/jbc.271.19.11284
58 https://doi.org/10.1074/jbc.273.41.26375
59 https://doi.org/10.1146/annurev.bb.06.060177.001055
60 https://doi.org/10.1146/annurev.bb.24.060195.000505
61 https://doi.org/10.1146/annurev.bb.25.060196.001115
62 schema:datePublished 2010-06
63 schema:datePublishedReg 2010-06-01
64 schema:description Traditional single site replacement mutations (in this case, phenylalanine to tyrosine) were compared with methods which exclusively employ (15)N and (19)F-edited two- and three-dimensional NMR experiments for purposes of assigning (19)F NMR resonances from calmodulin (CaM), biosynthetically labeled with 3-fluorophenylalanine (3-FPhe). The global substitution of 3-FPhe for native phenylalanine was tolerated in CaM as evidenced by a comparison of (1)H-(15)N HSQC spectra and calcium binding assays in the presence and absence of 3-FPhe. The (19)F NMR spectrum reveals six resolved resonances, one of which integrates to three 3-FPhe species, making for a total of eight fluorophenylalanines. Single phenylalanine to tyrosine mutants of five phenylalanine positions resulted in (19)F NMR spectra with significant chemical shift perturbations of the remaining resonances, and provided only a single definitive assignment. Although (1)H-(19)F heteronucleclear NOEs proved weak, (19)F-edited (1)H-(1)H NOESY connectivities were relatively easy to establish by making use of the (3)J(FH) coupling between the fluorine nucleus and the adjacent fluorophenylalanine delta proton. (19)F-edited NOESY connectivities between the delta protons and alpha and beta nuclei in addition to (15)N-edited (1)H, (1)H NOESY crosspeaks proved sufficient to assign 4 of 8 (19)F resonances. Controlled cleavage of the protein into two fragments using trypsin, and a repetition of the above 2D and 3D techniques resulted in unambiguous assignments of all 8 (19)F NMR resonances. Our studies suggest that (19)F-edited NOESY NMR spectra are generally adequate for complete assignment without the need to resort to mutational analysis.
65 schema:genre research_article
66 schema:inLanguage en
67 schema:isAccessibleForFree false
68 schema:isPartOf N2e037af5897a4a06b5153e64f1d96deb
69 N447ad98ea8684af09dd7dd012918f960
70 sg:journal.1101518
71 schema:name Approaches to the assignment of 19F resonances from 3-fluorophenylalanine labeled calmodulin using solution state NMR
72 schema:pagination 113-123
73 schema:productId N1a451f900b2046dcbcc0cebb73427b34
74 N30c4712ded53481083e49b96b9119024
75 N80ea3bc679614d3f86e37834635d2df5
76 N8f1dfc644bd24d24a933cfacf4728766
77 Nf50777cb8b17477caef329397c7b50d0
78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038016714
79 https://doi.org/10.1007/s10858-010-9415-y
80 schema:sdDatePublished 2019-04-11T10:17
81 schema:sdLicense https://scigraph.springernature.com/explorer/license/
82 schema:sdPublisher N63ed0a6f66c542df90d28fcaba0aa44f
83 schema:url http://link.springer.com/10.1007/s10858-010-9415-y
84 sgo:license sg:explorer/license/
85 sgo:sdDataset articles
86 rdf:type schema:ScholarlyArticle
87 N0fd1536335d3499bb1c0963e221242e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Nuclear Magnetic Resonance, Biomolecular
89 rdf:type schema:DefinedTerm
90 N104586b0ccb74502bf86ee4fdd63df24 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Fluorine
92 rdf:type schema:DefinedTerm
93 N120cbb6458a049bb8a8a4c21d2a86ce2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Solutions
95 rdf:type schema:DefinedTerm
96 N19efa5c2226245fea77639002698652b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Trypsin
98 rdf:type schema:DefinedTerm
99 N1a451f900b2046dcbcc0cebb73427b34 schema:name doi
100 schema:value 10.1007/s10858-010-9415-y
101 rdf:type schema:PropertyValue
102 N2e037af5897a4a06b5153e64f1d96deb schema:volumeNumber 47
103 rdf:type schema:PublicationVolume
104 N30c4712ded53481083e49b96b9119024 schema:name nlm_unique_id
105 schema:value 9110829
106 rdf:type schema:PropertyValue
107 N3e5afb03807c477d9e39a9f95ce7e5aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Tyrosine
109 rdf:type schema:DefinedTerm
110 N42bd22dfbd6b4150b5f7d88063055c14 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Calmodulin
112 rdf:type schema:DefinedTerm
113 N4394d6890bd845eca977cdba32b109a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Models, Molecular
115 rdf:type schema:DefinedTerm
116 N447ad98ea8684af09dd7dd012918f960 schema:issueNumber 2
117 rdf:type schema:PublicationIssue
118 N4768efaef1fc42619d74f40b21e3222f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Crystallography, X-Ray
120 rdf:type schema:DefinedTerm
121 N4b6946f2e6694a4ab54669d8a59427b2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Peptide Fragments
123 rdf:type schema:DefinedTerm
124 N52bcd408162e463cb6045234735e7177 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Mutagenesis, Site-Directed
126 rdf:type schema:DefinedTerm
127 N6290a67d47394e84ba92a4a5cead004e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Xenopus Proteins
129 rdf:type schema:DefinedTerm
130 N62dd3a2091e54b12be52eeb21d15b5ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Molecular Sequence Data
132 rdf:type schema:DefinedTerm
133 N63ed0a6f66c542df90d28fcaba0aa44f schema:name Springer Nature - SN SciGraph project
134 rdf:type schema:Organization
135 N80ea3bc679614d3f86e37834635d2df5 schema:name readcube_id
136 schema:value b519814ab60a65e7b1c6d08cd97103d3a593a8206f03c4590e43adf30266de1f
137 rdf:type schema:PropertyValue
138 N89d4586739b7407a8b2499522cc2fbee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Amino Acid Sequence
140 rdf:type schema:DefinedTerm
141 N8f1dfc644bd24d24a933cfacf4728766 schema:name dimensions_id
142 schema:value pub.1038016714
143 rdf:type schema:PropertyValue
144 N98068e87011d4bcd874418e7c1ba30b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Nitrogen Isotopes
146 rdf:type schema:DefinedTerm
147 Nb048a73543e74013aedd79457d37e47f rdf:first sg:person.01361657751.39
148 rdf:rest Nc26847294d424e85a17423b407e222b0
149 Nb56e5cacdd1040878f48c38efbc54328 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Phenylalanine
151 rdf:type schema:DefinedTerm
152 Nb704fb660cad4558ba5d7769048075dd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Animals
154 rdf:type schema:DefinedTerm
155 Nc26847294d424e85a17423b407e222b0 rdf:first sg:person.01171427402.14
156 rdf:rest rdf:nil
157 Nf50777cb8b17477caef329397c7b50d0 schema:name pubmed_id
158 schema:value 20401735
159 rdf:type schema:PropertyValue
160 Nfb8409fdf59c4960b53564a5c1d5910f rdf:first sg:person.01220465703.40
161 rdf:rest Nb048a73543e74013aedd79457d37e47f
162 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
163 schema:name Biological Sciences
164 rdf:type schema:DefinedTerm
165 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
166 schema:name Biochemistry and Cell Biology
167 rdf:type schema:DefinedTerm
168 sg:journal.1101518 schema:issn 0925-2738
169 1573-5001
170 schema:name Journal of Biomolecular NMR
171 rdf:type schema:Periodical
172 sg:person.01171427402.14 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
173 schema:familyName Scott Prosser
174 schema:givenName R.
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171427402.14
176 rdf:type schema:Person
177 sg:person.01220465703.40 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
178 schema:familyName Kitevski-LeBlanc
179 schema:givenName Julianne L.
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220465703.40
181 rdf:type schema:Person
182 sg:person.01361657751.39 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
183 schema:familyName Evanics
184 schema:givenName Ferenc
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361657751.39
186 rdf:type schema:Person
187 sg:pub.10.1007/bf00185455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041846678
188 https://doi.org/10.1007/bf00185455
189 rdf:type schema:CreativeWork
190 sg:pub.10.1007/bf00197809 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001469691
191 https://doi.org/10.1007/bf00197809
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/bf00404272 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004332552
194 https://doi.org/10.1007/bf00404272
195 rdf:type schema:CreativeWork
196 sg:pub.10.1007/s10858-004-3534-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028093136
197 https://doi.org/10.1007/s10858-004-3534-2
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/nsb0995-758 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026576907
200 https://doi.org/10.1038/nsb0995-758
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/nsb0995-777 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033189599
203 https://doi.org/10.1038/nsb0995-777
204 rdf:type schema:CreativeWork
205 https://app.dimensions.ai/details/publication/pub.1077812513 schema:CreativeWork
206 https://app.dimensions.ai/details/publication/pub.1077991809 schema:CreativeWork
207 https://app.dimensions.ai/details/publication/pub.1082839909 schema:CreativeWork
208 https://doi.org/10.1002/1097-458x(200007)38:7<551::aid-mrc686>3.0.co;2-q schema:sameAs https://app.dimensions.ai/details/publication/pub.1020957930
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1002/cmr.10035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026082447
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1006/abio.2002.5661 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000806680
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1006/jmbi.1998.1935 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000010976
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1006/jmbi.1999.3188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001931600
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1006/snmr.2002.0063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029960993
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/0005-2744(77)90199-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001576498
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/0006-2952(90)90190-v schema:sameAs https://app.dimensions.ai/details/publication/pub.1018188671
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/0079-6565(94)80009-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051298558
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/j.bbagen.2006.10.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012292357
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.jmb.2004.12.058 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034801867
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/j.str.2003.09.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048409736
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/s0022-2836(02)00465-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041550713
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/s0092-8674(02)00682-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007001088
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/s0959-440x(00)00234-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037648308
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/s0969-2126(00)00544-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014373103
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1021/bi000582x schema:sameAs https://app.dimensions.ai/details/publication/pub.1055159139
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1021/bi00138a005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055162267
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1021/bi9628275 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055213215
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1021/ja00076a099 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055704798
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1021/ja00087a058 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055705760
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1021/ja00353a071 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055725893
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1021/ja0041876 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055730900
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1021/ja060618u schema:sameAs https://app.dimensions.ai/details/publication/pub.1055841592
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1021/ja8085752 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055860044
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1021/ja960465i schema:sameAs https://app.dimensions.ai/details/publication/pub.1055864741
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1073/pnas.0308641101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051783879
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1073/pnas.0705253104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005534760
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1074/jbc.271.19.11284 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011769863
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1074/jbc.273.41.26375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046767462
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1146/annurev.bb.06.060177.001055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027823832
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1146/annurev.bb.24.060195.000505 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015489796
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1146/annurev.bb.25.060196.001115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020433849
273 rdf:type schema:CreativeWork
274 https://www.grid.ac/institutes/grid.17063.33 schema:alternateName University of Toronto
275 schema:name Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, ON, Canada
276 Department of Chemistry, University of Toronto, UTM, 3359 Mississauga Rd. North, L5L 1C6, Mississauga, ON, Canada
277 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...