Resolution enhanced homonuclear carbon decoupled triple resonance experiments for unambiguous RNA structural characterization View Full Text


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Article Info

DATE

2005-06

AUTHORS

Kwaku T. Dayie

ABSTRACT

Large RNAs (>30 nucleotides) suffer from extensive resonance overlap that can seriously hamper unambiguous structural characterization. Here we present a set of 3D multinuclear NMR experiments with improved and optimized resolution and sensitivity for aiding with the assignment of RNA molecules. In all these experiments strong base and ribose carbon-carbon couplings are eliminated by homonuclear band-selective decoupling, leading to improved signal to noise and resolution of the C5, C6, and C1' carbon resonances. This decoupling scheme is applied to base-type selective 13C-edited NOESY, 13C-edited TOCSY (HCCH, CCH), HCCNH, and ribose H1C1C2 experiments. The 3D implementation of the HCCNH experiment with both carbon and nitrogen evolution enables direct correlation of 13C and 15N resonances at different proton resonant frequencies. The advantages of the new experiments are demonstrated on a 36 nucleotides hairpin RNA from domain 5 (D5) of the group II intron Pylaiella littoralis using an abbreviated assignment strategy. These four experiments provided additional separation for regions of the RNA that have overlapped chemical shift resonances, and enabled the assignment of critical D5 bulge nucleotides that could not be assigned using current experimental schemes. More... »

PAGES

129-139

References to SciGraph publications

  • 1997-04. Assignment methodology for larger RNA oligonucleotides: Application to an ATP-binding RNA aptamer in JOURNAL OF BIOMOLECULAR NMR
  • 2001-06. Novel 2D and 3D multiple-quantum bi-directional HCNCH experiments for the correlation of ribose and base protons/carbons in 13C/15N labeled RNA in JOURNAL OF BIOMOLECULAR NMR
  • 1995-12. Triple resonance HNCCCH experiments for correlating exchangeable and nonexchangeable cytidine and uridine base protons in RNA in JOURNAL OF BIOMOLECULAR NMR
  • 2001-06. A TROSY relayed HCCH-COSY experiment for correlating adenine H2/H8 resonances in uniformly 13C-labeled RNA molecules in JOURNAL OF BIOMOLECULAR NMR
  • 2003-05. Triple resonance experiments for the simultaneous correlation of H6/H5 and exchangeable protons of pyrimidine nucleotides in 13C,15N-labeled RNA applicable to larger RNA molecules in JOURNAL OF BIOMOLECULAR NMR
  • 2004-02. Solution structure of domain 5 of a group II intron ribozyme reveals a new RNA motif in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2004-09. Enhanced spectral resolution in RNA HCP spectra for measurement of 3JC2′P and 3JC4′P couplings and 31P chemical shift changes upon weak alignment in JOURNAL OF BIOMOLECULAR NMR
  • 1992-11. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions in JOURNAL OF BIOMOLECULAR NMR
  • 1995-11. NMRPipe: A multidimensional spectral processing system based on UNIX pipes in JOURNAL OF BIOMOLECULAR NMR
  • 2001-02. Base-type-selective high-resolution 13C edited NOESY for sequential assignment of large RNAs in JOURNAL OF BIOMOLECULAR NMR
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10858-005-5093-6

    DOI

    http://dx.doi.org/10.1007/s10858-005-5093-6

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1034608770

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16034664


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    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10858-005-5093-6'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10858-005-5093-6'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10858-005-5093-6'

    RDF/XML is a standard XML format for linked data.

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