In vitro study of partially hydrolyzed poly(2-ethyl-2-oxazolines) as materials for biomedical applications View Full Text


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Article Info

DATE

2015-04

AUTHORS

Rushita Shah, Zuzana Kronekova, Anna Zahoranová, Ladislav Roller, Nabanita Saha, Petr Saha, Juraj Kronek

ABSTRACT

Polymers based on 2-oxazoline, such as poly(2-ethyl-2-oxazolines) (PETOx), are considered to be a type of 'pseudopeptide' with the ability to form novel biomaterials. The hydrolysis of PETOx was carried out to evaluate its use in biomedical applications. In the present work, PETOx samples with a range of molar masses were prepared by living cationic polymerization. Hydrolysis was carried out at time intervals ranging from 15 to 180 min to prepare copolymers with different amounts of ethylene imine units. (1)H NMR spectroscopy was used to identify the structure of the hydrolyzed polymers. The dependence of in vitro cell viability on the degree of hydrolysis was determined using three different model cell lines, namely, mouse embryonic 3T3 fibroblasts, pancreatic βTC3 cells, and mouse lymphoid macrophages P388.D1. It was demonstrated that increasing the degree of hydrolysis decreased cell viability for all cell types. Fibroblast cells displayed the highest tolerance; additionally, the effect of polymer size showed no observable significance. Macrophage cells, immune system representatives, displayed the highest sensitivity to contact with hydrolyzed PETOx. The effect of polymer hydrolysis, polymer concentration and the incubation time on cell viability was experimentally observed. Confocal laser-scanning microscopy provided evidence of cellular uptake of pyrene-labeled (co)polymers. More... »

PAGES

157

References to SciGraph publications

  • 2012-06. Immunomodulatory efficiency of poly(2-oxazolines) in JOURNAL OF MATERIALS SCIENCE: MATERIALS IN MEDICINE
  • 2010-12. Nanomaterial cytotoxicity is composition, size, and cell type dependent in PARTICLE AND FIBRE TOXICOLOGY
  • 2011-07. In vitro bio-immunological and cytotoxicity studies of poly(2-oxazolines) in JOURNAL OF MATERIALS SCIENCE: MATERIALS IN MEDICINE
  • 2010-03. Synthesis and bioimmunological efficiency of poly(2-oxazolines) containing a free amino group in JOURNAL OF MATERIALS SCIENCE: MATERIALS IN MEDICINE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10856-015-5485-4

    DOI

    http://dx.doi.org/10.1007/s10856-015-5485-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1020167162

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25783502


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