Biomimetic design and fabrication of porous chitosan–gelatin liver scaffolds with hierarchical channel network View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-01

AUTHORS

Haibo Gong, Jephte Agustin, David Wootton, Jack G. Zhou

ABSTRACT

The presence of a hierarchical channel network in tissue engineering scaffold is essential to construct metabolically demanding liver tissue with thick and complex structures. In this research, chitosan–gelatin (C/G) scaffolds with fine three-dimensional channels were fabricated using indirect solid freeform fabrication and freeze-drying techniques. Fabrication processes were studied to create predesigned hierarchical channel network inside C/G scaffolds and achieve desired porous structure. Static in-vitro cell culture test showed that HepG2 cells attached on both micro-pores and micro-channels in C/G scaffolds successfully. HepG2 proliferated at much higher rates on C/G scaffolds with channel network, compared with those without channels. This approach demonstrated a promising way to engineer liver scaffolds with hierarchical channel network, and may lead to the development of thick and complex liver tissue equivalent in the future. More... »

PAGES

113-120

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10856-013-5061-8

DOI

http://dx.doi.org/10.1007/s10856-013-5061-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004391336

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24121873


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