Preparation of polycarbonate/gelatine microspheres using a high-voltage electrostatic technique for enhancing the adhesion and proliferation of mesenchymal stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-05

AUTHORS

Chunxu Li, Linlong Li, Rui Ma, Zongliang Wang, Xincui Shi, Xiaoyu Yang, Yu Wang, Peibiao Zhang

ABSTRACT

In this study, a high-voltage electrostatic technique is introduced to prepare polycarbonate (PC) microspheres in order to design an expansion strategy for the bone marrow mesenchymal stem cells (BMMSCs). The effects of the solution concentration, temperature, nozzle specification and voltage on the sphericity, homogeneity, diameter and sedimentation velocities of the microspheres are investigated. By optimising the preparation parameters, PC microspheres with a diameter of 316.39 μm ± 14.75 μm and porous surface were prepared. The gelatine-modified PC (GEL-PC) microspheres exhibited better hydrophilicity and protein adsorption ability than PC microspheres. Enhancement of the adhesion and proliferation of the BMMSCs can be observed on GEL-PC microspheres after 7 days. Therefore, this study demonstrates that employing a dynamic culture using GEL-PC microspheres is a promising method for deriving BMMSCs. More... »

PAGES

1-18

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10853-018-03200-1

DOI

http://dx.doi.org/10.1007/s10853-018-03200-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111754464


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