Biobanking of different body fluids within the frame of IVF—a standard operating procedure to improve reproductive biology research View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-11-26

AUTHORS

Michael Schenk, Berthold Huppertz, Barbara Obermayer-Pietsch, Darja Kastelic, Martina Hörmann-Kröpfl, Gregor Weiss

ABSTRACT

PurposeThe aim of the present study was to develop a standard operating procedure (SOP) for the collection, transport, and storage of human cumulus cells, follicular fluid, blood serum, seminal plasma, embryo culture supernatant, and embryo culture supernatant control obtained within the IVF process under approved protocols and written informed consent from participating patients. The SOP was developed at the Kinderwunsch Institut Schenk, Dobl, Austria, together with Biobank Graz of the Medical University of Graz, Austria.MethodsThe SOP provides comprehensive details of laboratory procedures and sampling of the different fluids within the IVF process. Furthermore, information on sample coding, references of involved laboratory techniques (e.g., oocyte retrieval with a Steiner-TAN needle), ethical approvals, and biobanking procedures are presented.ResultsThe result of the present study is a standard operating procedure.ConclusionsThe SOP ensures a professional way for collection and scientific use of IVF samples by the Kinderwunsch Institut Schenk, Dobl, Austria, and Biobank Graz of the Medical University of Graz, Austria. It can be used as a template for other institutions to unify specimen collection procedures in the field of reproductive health research. More... »

PAGES

283-290

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10815-016-0847-5

DOI

http://dx.doi.org/10.1007/s10815-016-0847-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022098111

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27889868


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