Ontology type: schema:ScholarlyArticle Open Access: True
2015-11
AUTHORSD. Montjean, C. Geoffroy-Siraudin, M. Gervoise-Boyer, P. Tourame, P. Boyer
ABSTRACTPURPOSE: Oocyte vitrification is a worldwide used technique that has proved its worth. Although it was shown not to alter oocyte integrity, a recent study concluded that it may affect oocyte embryo development. As the morphology and kinetics of embryos derived from sibling fresh and vitrified oocytes have not been described previously, the present study evaluates cleavage rate, blastomeres size, fragmentation rate, and blastocyst formation in vitrified/warmed oocyte derived embryos (VODE) as compared with sibling fresh oocytes derived embryos (FODE). METHODS: This investigation included 90 infertility cases displaying large cohort of mature oocytes at pick up, divided into 2 groups after denudation. A part of oocytes underwent ICSI while others were vitrified. Oocyte warming cycles were performed when no pregnancy was achieved using fresh eggs. Zygote to blastocyst development was recorded prospectively in an image database up to day 5. RESULTS: VODE did not show major difference as compared with FODE in terms of cleavage rate, number of blastomeres, fragmentation rate, and blastomeres size. Furthermore, percentage of morulae at day 4 and blastocysts at day 5 are not affected by oocyte vitrification. CONCLUSION: Although our results show that embryo development is not altered by oocyte vitrification, offspring follow-up is essential to exclude any adverse developmental effect of the technique. More... »
PAGES1615-1621
http://scigraph.springernature.com/pub.10.1007/s10815-015-0569-0
DOIhttp://dx.doi.org/10.1007/s10815-015-0569-0
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/26409475
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