Morphokinetics analysis of embryos derived from vitrified/warmed oocytes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-11

AUTHORS

D. Montjean, C. Geoffroy-Siraudin, M. Gervoise-Boyer, P. Tourame, P. Boyer

ABSTRACT

PURPOSE: Oocyte vitrification is a worldwide used technique that has proved its worth. Although it was shown not to alter oocyte integrity, a recent study concluded that it may affect oocyte embryo development. As the morphology and kinetics of embryos derived from sibling fresh and vitrified oocytes have not been described previously, the present study evaluates cleavage rate, blastomeres size, fragmentation rate, and blastocyst formation in vitrified/warmed oocyte derived embryos (VODE) as compared with sibling fresh oocytes derived embryos (FODE). METHODS: This investigation included 90 infertility cases displaying large cohort of mature oocytes at pick up, divided into 2 groups after denudation. A part of oocytes underwent ICSI while others were vitrified. Oocyte warming cycles were performed when no pregnancy was achieved using fresh eggs. Zygote to blastocyst development was recorded prospectively in an image database up to day 5. RESULTS: VODE did not show major difference as compared with FODE in terms of cleavage rate, number of blastomeres, fragmentation rate, and blastomeres size. Furthermore, percentage of morulae at day 4 and blastocysts at day 5 are not affected by oocyte vitrification. CONCLUSION: Although our results show that embryo development is not altered by oocyte vitrification, offspring follow-up is essential to exclude any adverse developmental effect of the technique. More... »

PAGES

1615-1621

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10815-015-0569-0

DOI

http://dx.doi.org/10.1007/s10815-015-0569-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031824138

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26409475


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