Emission Anisotropy of Fluorescein Covalently Linked to Oligonucleotides View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-03

AUTHORS

A. P. Blokhin, M. V. Kvach, V. A. Povedailo, V. V. Shmanai, D. L. Yakovlev

ABSTRACT

Rotational depolarization of fluorescence from fluorescein covalently linked to oligonucleotides was studied. Fluorescence anisotropy of two nucleic acids was measured as a function of the temperature-to-viscosity ratio in buffer solutions with various glycerin concentrations. It was shown that the experimental results could be satisfactorily explained by a diffusion model of an elongated molecular top with internal rotation. It was found that the coefficient of internal rotational diffusion in all instances was 1.5–2 times greater than that for rotation around the oligonucleotide axis. More... »

PAGES

19-24

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10812-017-0420-8

DOI

http://dx.doi.org/10.1007/s10812-017-0420-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084815357


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0912", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Materials Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Engineering", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "BI Stepanov Institute of Physics", 
          "id": "https://www.grid.ac/institutes/grid.426545.4", 
          "name": [
            "B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Nezavisimost\u2032 Ave, 220072, Minsk, Belarus"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Blokhin", 
        "givenName": "A. P.", 
        "id": "sg:person.016301203567.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016301203567.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Physical and Organic Chemistry", 
          "id": "https://www.grid.ac/institutes/grid.435325.6", 
          "name": [
            "Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kvach", 
        "givenName": "M. V.", 
        "id": "sg:person.01007165027.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007165027.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "BI Stepanov Institute of Physics", 
          "id": "https://www.grid.ac/institutes/grid.426545.4", 
          "name": [
            "B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Nezavisimost\u2032 Ave, 220072, Minsk, Belarus"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Povedailo", 
        "givenName": "V. A.", 
        "id": "sg:person.07522503641.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07522503641.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Physical and Organic Chemistry", 
          "id": "https://www.grid.ac/institutes/grid.435325.6", 
          "name": [
            "Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shmanai", 
        "givenName": "V. V.", 
        "id": "sg:person.0656057035.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656057035.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "BI Stepanov Institute of Physics", 
          "id": "https://www.grid.ac/institutes/grid.426545.4", 
          "name": [
            "B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Nezavisimost\u2032 Ave, 220072, Minsk, Belarus"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yakovlev", 
        "givenName": "D. L.", 
        "id": "sg:person.014100067442.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014100067442.38"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1063/1.4812637", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002162811"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj0750345", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005037123"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj0750345", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005037123"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj0510155", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005074965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj0510155", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005074965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1529/biophysj.105.069500", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014845652"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-0-387-46312-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025074156", 
          "https://doi.org/10.1007/978-0-387-46312-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-0-387-46312-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025074156", 
          "https://doi.org/10.1007/978-0-387-46312-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1037402207", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-36705-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037402207", 
          "https://doi.org/10.1007/978-3-642-36705-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-36705-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037402207", 
          "https://doi.org/10.1007/978-3-642-36705-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0301-0104(88)87001-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044845283"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9610919", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055212192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9610919", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055212192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ie50501a040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055632015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.1701790", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057772143"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.432951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058010983"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.447378", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058025398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.473708", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058055351"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.481453", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058079274"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-03", 
    "datePublishedReg": "2017-03-01", 
    "description": "Rotational depolarization of fluorescence from fluorescein covalently linked to oligonucleotides was studied. Fluorescence anisotropy of two nucleic acids was measured as a function of the temperature-to-viscosity ratio in buffer solutions with various glycerin concentrations. It was shown that the experimental results could be satisfactorily explained by a diffusion model of an elongated molecular top with internal rotation. It was found that the coefficient of internal rotational diffusion in all instances was 1.5\u20132 times greater than that for rotation around the oligonucleotide axis.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10812-017-0420-8", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1005931", 
        "issn": [
          "0021-9037", 
          "1573-8647"
        ], 
        "name": "Journal of Applied Spectroscopy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "84"
      }
    ], 
    "name": "Emission Anisotropy of Fluorescein Covalently Linked to Oligonucleotides", 
    "pagination": "19-24", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "cac96db6ae87e69d06e01987ca25151dfe3cb737223b6687e21224b872233495"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10812-017-0420-8"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084815357"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10812-017-0420-8", 
      "https://app.dimensions.ai/details/publication/pub.1084815357"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:29", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000349_0000000349/records_113641_00000004.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs10812-017-0420-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10812-017-0420-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10812-017-0420-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10812-017-0420-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10812-017-0420-8'


 

This table displays all metadata directly associated to this object as RDF triples.

138 TRIPLES      21 PREDICATES      42 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10812-017-0420-8 schema:about anzsrc-for:09
2 anzsrc-for:0912
3 schema:author N19168b8c7ea440dc8d675c37457bfac4
4 schema:citation sg:pub.10.1007/978-0-387-46312-4
5 sg:pub.10.1007/978-3-642-36705-2
6 https://app.dimensions.ai/details/publication/pub.1037402207
7 https://doi.org/10.1016/0301-0104(88)87001-0
8 https://doi.org/10.1021/bi9610919
9 https://doi.org/10.1021/ie50501a040
10 https://doi.org/10.1042/bj0510155
11 https://doi.org/10.1042/bj0750345
12 https://doi.org/10.1063/1.1701790
13 https://doi.org/10.1063/1.432951
14 https://doi.org/10.1063/1.447378
15 https://doi.org/10.1063/1.473708
16 https://doi.org/10.1063/1.4812637
17 https://doi.org/10.1063/1.481453
18 https://doi.org/10.1529/biophysj.105.069500
19 schema:datePublished 2017-03
20 schema:datePublishedReg 2017-03-01
21 schema:description Rotational depolarization of fluorescence from fluorescein covalently linked to oligonucleotides was studied. Fluorescence anisotropy of two nucleic acids was measured as a function of the temperature-to-viscosity ratio in buffer solutions with various glycerin concentrations. It was shown that the experimental results could be satisfactorily explained by a diffusion model of an elongated molecular top with internal rotation. It was found that the coefficient of internal rotational diffusion in all instances was 1.5–2 times greater than that for rotation around the oligonucleotide axis.
22 schema:genre research_article
23 schema:inLanguage en
24 schema:isAccessibleForFree false
25 schema:isPartOf N4ff90325afbd43e9bd01691bfe8f03c5
26 N6a77144bf14d44a28e75b6fae86a4d84
27 sg:journal.1005931
28 schema:name Emission Anisotropy of Fluorescein Covalently Linked to Oligonucleotides
29 schema:pagination 19-24
30 schema:productId N2cdf6eb0eb3643d296c06559738188d4
31 N8f0210d5080d4fa49317375f4eea3951
32 Na0a70f949e7b4ccfb161ba21ffd71a5a
33 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084815357
34 https://doi.org/10.1007/s10812-017-0420-8
35 schema:sdDatePublished 2019-04-11T10:29
36 schema:sdLicense https://scigraph.springernature.com/explorer/license/
37 schema:sdPublisher Nd51f68913338488f8efb2a11bf25c838
38 schema:url https://link.springer.com/10.1007%2Fs10812-017-0420-8
39 sgo:license sg:explorer/license/
40 sgo:sdDataset articles
41 rdf:type schema:ScholarlyArticle
42 N19168b8c7ea440dc8d675c37457bfac4 rdf:first sg:person.016301203567.99
43 rdf:rest N1d2cf9b3e4b043a086a4bfa425ed3900
44 N1d2cf9b3e4b043a086a4bfa425ed3900 rdf:first sg:person.01007165027.54
45 rdf:rest N44c62f63334043d8a2b97dd3f1a5aa25
46 N2cdf6eb0eb3643d296c06559738188d4 schema:name dimensions_id
47 schema:value pub.1084815357
48 rdf:type schema:PropertyValue
49 N44c62f63334043d8a2b97dd3f1a5aa25 rdf:first sg:person.07522503641.48
50 rdf:rest N672ef072b7c74dcd91429ce6f67ecc87
51 N4ff90325afbd43e9bd01691bfe8f03c5 schema:volumeNumber 84
52 rdf:type schema:PublicationVolume
53 N5a560139c57a4db7b889f5ca035347ad rdf:first sg:person.014100067442.38
54 rdf:rest rdf:nil
55 N672ef072b7c74dcd91429ce6f67ecc87 rdf:first sg:person.0656057035.12
56 rdf:rest N5a560139c57a4db7b889f5ca035347ad
57 N6a77144bf14d44a28e75b6fae86a4d84 schema:issueNumber 1
58 rdf:type schema:PublicationIssue
59 N8f0210d5080d4fa49317375f4eea3951 schema:name doi
60 schema:value 10.1007/s10812-017-0420-8
61 rdf:type schema:PropertyValue
62 Na0a70f949e7b4ccfb161ba21ffd71a5a schema:name readcube_id
63 schema:value cac96db6ae87e69d06e01987ca25151dfe3cb737223b6687e21224b872233495
64 rdf:type schema:PropertyValue
65 Nd51f68913338488f8efb2a11bf25c838 schema:name Springer Nature - SN SciGraph project
66 rdf:type schema:Organization
67 anzsrc-for:09 schema:inDefinedTermSet anzsrc-for:
68 schema:name Engineering
69 rdf:type schema:DefinedTerm
70 anzsrc-for:0912 schema:inDefinedTermSet anzsrc-for:
71 schema:name Materials Engineering
72 rdf:type schema:DefinedTerm
73 sg:journal.1005931 schema:issn 0021-9037
74 1573-8647
75 schema:name Journal of Applied Spectroscopy
76 rdf:type schema:Periodical
77 sg:person.01007165027.54 schema:affiliation https://www.grid.ac/institutes/grid.435325.6
78 schema:familyName Kvach
79 schema:givenName M. V.
80 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007165027.54
81 rdf:type schema:Person
82 sg:person.014100067442.38 schema:affiliation https://www.grid.ac/institutes/grid.426545.4
83 schema:familyName Yakovlev
84 schema:givenName D. L.
85 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014100067442.38
86 rdf:type schema:Person
87 sg:person.016301203567.99 schema:affiliation https://www.grid.ac/institutes/grid.426545.4
88 schema:familyName Blokhin
89 schema:givenName A. P.
90 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016301203567.99
91 rdf:type schema:Person
92 sg:person.0656057035.12 schema:affiliation https://www.grid.ac/institutes/grid.435325.6
93 schema:familyName Shmanai
94 schema:givenName V. V.
95 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656057035.12
96 rdf:type schema:Person
97 sg:person.07522503641.48 schema:affiliation https://www.grid.ac/institutes/grid.426545.4
98 schema:familyName Povedailo
99 schema:givenName V. A.
100 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07522503641.48
101 rdf:type schema:Person
102 sg:pub.10.1007/978-0-387-46312-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025074156
103 https://doi.org/10.1007/978-0-387-46312-4
104 rdf:type schema:CreativeWork
105 sg:pub.10.1007/978-3-642-36705-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037402207
106 https://doi.org/10.1007/978-3-642-36705-2
107 rdf:type schema:CreativeWork
108 https://app.dimensions.ai/details/publication/pub.1037402207 schema:CreativeWork
109 https://doi.org/10.1016/0301-0104(88)87001-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044845283
110 rdf:type schema:CreativeWork
111 https://doi.org/10.1021/bi9610919 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055212192
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1021/ie50501a040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055632015
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1042/bj0510155 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005074965
116 rdf:type schema:CreativeWork
117 https://doi.org/10.1042/bj0750345 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005037123
118 rdf:type schema:CreativeWork
119 https://doi.org/10.1063/1.1701790 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057772143
120 rdf:type schema:CreativeWork
121 https://doi.org/10.1063/1.432951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058010983
122 rdf:type schema:CreativeWork
123 https://doi.org/10.1063/1.447378 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058025398
124 rdf:type schema:CreativeWork
125 https://doi.org/10.1063/1.473708 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058055351
126 rdf:type schema:CreativeWork
127 https://doi.org/10.1063/1.4812637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002162811
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1063/1.481453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058079274
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1529/biophysj.105.069500 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014845652
132 rdf:type schema:CreativeWork
133 https://www.grid.ac/institutes/grid.426545.4 schema:alternateName BI Stepanov Institute of Physics
134 schema:name B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Nezavisimost′ Ave, 220072, Minsk, Belarus
135 rdf:type schema:Organization
136 https://www.grid.ac/institutes/grid.435325.6 schema:alternateName Institute of Physical and Organic Chemistry
137 schema:name Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus
138 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...