The red microalga Flintiella sanguinaria as a new exopolysaccharide producer View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-10

AUTHORS

Clément Gaignard, Vanessa Macao, Christine Gardarin, Christophe Rihouey, Luc Picton, Philippe Michaud, Céline Laroche

ABSTRACT

Flintiella sanguinaria is a red unicellular microalgae belonging to Porphyridiophyceae with a high potential as an exopolysaccharide producer. A new culture medium was developed by a stoichiometry approach based on elemental analysis of microalgae, and culture conditions were improved after measurements of photosynthetic activity as a function of irradiance, temperature and pH. These optimal conditions were thus validated in a photobioreactor for the production of biomass and exopolysaccharide (EPS). After extraction and purification of the EPS, it was characterised by HPAEC-PAD, HPLC and FT-IR as a methylated and acetylated galactoxylan including a significant amount of rhamnose and glucuronic acid in its structure. Macromolecular conformation in dilute solution of native, deproteinised and desubstituted EPS from F. sanguinaria revealed that this polysaccharide had a strong associative behaviour in which hydrophobic interactions or hydrogen bonding but also proteins were implicated. More... »

PAGES

2803-2814

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10811-018-1389-2

DOI

http://dx.doi.org/10.1007/s10811-018-1389-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1100790541


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