Electropolymerization of hydroxyphenylacetic acid isomers and the development of a bioelectrode for the diagnosis of bacterial meningitis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-12

AUTHORS

Luciano Pereira Rodrigues, Deusmaque Carneiro Ferreira, Lucas Franco Ferreira, Sara Cuadros-Orellana, Guilherme Corrêa de Oliveira, Ana G. Brito-Madurro, Ronaldo Junio de Oliveira, Odonírio Abrahão, João M. Madurro

ABSTRACT

In this work, three polymeric films derived from hydroxyphenylacetic acid isomers were electropolymerized onto the surfaces of graphite electrodes through cyclic voltammetry. Analysis of electrochemical behaviors and electrical properties of the three polymeric films were investigated and their probably structures were explored by molecular modeling. The best functionalization strategy for the incorporation and retention of adenine and guanine nitrogenous bases was the poly(3-hydroxyphenylacetic acid). It was applied for the immobilization of a nucleotide probe specific to DNA of the bacteria Neisseria meningitidis operated in amperometric and impedimetric indirect modes. The simple protocol of electrochemical detection of DNA by the bioelectrode, developed in this work, could be further enhanced and applied in a low-cost and pain-less platform to diagnose human meningitis infection. More... »

PAGES

1277-1287

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10800-015-0892-2

DOI

http://dx.doi.org/10.1007/s10800-015-0892-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047826301


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