Distribution of sperm antigen 6 (SPAG6) and 16 (SPAG16) in mouse ciliated and non-ciliated tissues View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-03-25

AUTHORS

Jimena Alciaturi, Gabriel Anesetti, Florencia Irigoin, Fernanda Skowronek, Rossana Sapiro

ABSTRACT

The cilia and flagella of eukaryotic cells serve many functions, exhibiting remarkable conservation of both structure and molecular composition in widely divergent eukaryotic organisms. SPAG6 and SPAG16 are the homologous in the mice to Chlamydomonas reinhardtii PF16 and PF20. Both proteins are associated with the axonemal central apparatus and are essential for ciliary and flagellar motility in mammals. Recent data derived from high-throughput studies revealed expression of these genes in tissues that do not contain motile cilia. However, the distribution of SPAG6 and SPAG16 in ciliated and non-ciliated tissues is not completely understood. In this work, we performed a quantitative analysis of the expression of Spag6 and Spag16 genes in parallel with the immune-localization of the proteins in several tissues of adult mice. Expression of mRNA was higher in the testis and tissues bearing motile cilia than in the other analyzed tissues. Both proteins were present in ciliated and non-ciliated tissues. In the testis, SPAG6 was detected in spermatogonia, spermatocytes, and in the sperm flagella whereas SPAG16 was found in spermatocytes and in the sperm flagella. In addition, both proteins were detected in the cytoplasm of cells from the brain, spinal cord, and ovary. A small isoform of SPAG16 was localized in the nucleus of germ cells and some neurons. In a parallel set of experiments, we overexpressed EGFP-SPAG6 in cultured cells and observed that the protein co-localized with a subset of acetylated cytoplasmic microtubules. A role of these proteins stabilizing the cytoplasmic microtubules of eukaryotic cells is discussed. More... »

PAGES

1-14

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10735-019-09817-z

DOI

http://dx.doi.org/10.1007/s10735-019-09817-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112985219

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30911868


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of the Republic", 
          "id": "https://www.grid.ac/institutes/grid.11630.35", 
          "name": [
            "Departamento de Histolog\u00eda y Embriolog\u00eda, Facultad de Medicina, Universidad de la Rep\u00fablica, Gral. Flores 2125, Montevideo, Uruguay"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alciaturi", 
        "givenName": "Jimena", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of the Republic", 
          "id": "https://www.grid.ac/institutes/grid.11630.35", 
          "name": [
            "Departamento de Histolog\u00eda y Embriolog\u00eda, Facultad de Medicina, Universidad de la Rep\u00fablica, Gral. Flores 2125, Montevideo, Uruguay"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Anesetti", 
        "givenName": "Gabriel", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur de Montevideo", 
          "id": "https://www.grid.ac/institutes/grid.418532.9", 
          "name": [
            "Departamento de Histolog\u00eda y Embriolog\u00eda, Facultad de Medicina, Universidad de la Rep\u00fablica, Gral. Flores 2125, Montevideo, Uruguay", 
            "Laboratorio de Gen\u00e9tica Molecular Humana, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, Uruguay"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Irigoin", 
        "givenName": "Florencia", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of the Republic", 
          "id": "https://www.grid.ac/institutes/grid.11630.35", 
          "name": [
            "Departamento de Histolog\u00eda y Embriolog\u00eda, Facultad de Medicina, Universidad de la Rep\u00fablica, Gral. Flores 2125, Montevideo, Uruguay"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Skowronek", 
        "givenName": "Fernanda", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of the Republic", 
          "id": "https://www.grid.ac/institutes/grid.11630.35", 
          "name": [
            "Departamento de Histolog\u00eda y Embriolog\u00eda, Facultad de Medicina, Universidad de la Rep\u00fablica, Gral. Flores 2125, Montevideo, Uruguay"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sapiro", 
        "givenName": "Rossana", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1095/biolreprod62.3.511", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002240647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod62.3.511", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002240647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tcb.2014.10.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004236310"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.22.17.6298-6305.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004576014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.22.22.7993-8004.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006134435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cm.21271", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007902797"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep25840", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010745449", 
          "https://doi.org/10.1038/srep25840"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.1999.9520", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011594986"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/cbir.1996.0011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013003822"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/molehr/gah163", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013882446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1165/rcmb.2012-0362oc", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015998056"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tcb.2005.04.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016257468"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tcb.2005.04.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016257468"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m300688200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020795036"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0404280101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021692115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.050377", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023933181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.physiol.69.040705.141236", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025273003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0008189", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026311398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0810777105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026670084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026706357", 
          "https://doi.org/10.1038/nmeth.2019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cm.20189", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027412755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13630-016-0030-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027428285", 
          "https://doi.org/10.1186/s13630-016-0030-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027621591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027621591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0906414106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028495935"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pcbi.1001065", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029034776"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0020625", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029088213"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10735-016-9694-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029455653", 
          "https://doi.org/10.1007/s10735-016-9694-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10735-016-9694-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029455653", 
          "https://doi.org/10.1007/s10735-016-9694-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.74.1.68", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029870095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.051326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031173269"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0112879", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031542095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/356080a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032052993", 
          "https://doi.org/10.1038/356080a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.100.6.2008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034426016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0107271", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034806526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1439-0264.2010.01000.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036162027"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1439-0264.2010.01000.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036162027"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0125936", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037521128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1078-0432.ccr-05-2552", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041779391"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(75)90411-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041951841"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-pharmtox-011112-140326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042629305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/mcp.m400177-mcp200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043986155"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.104.2.289", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046168581"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.103.2.571", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046191493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/cji.0b013e3181fb64fa", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047705689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/cji.0b013e3181fb64fa", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047705689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep16506", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048660641", 
          "https://doi.org/10.1038/srep16506"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.105.049254", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052243280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0169(1997)38:1<1::aid-cm1>3.0.co;2-c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052943932"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.91.3.125s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058438183"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.115.130989", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060111067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/ijo.2014.2768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071513745"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/ijo.2014.2768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071513745"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079207378", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13630-017-0046-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084252548", 
          "https://doi.org/10.1186/s13630-017-0046-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13630-017-0046-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084252548", 
          "https://doi.org/10.1186/s13630-017-0046-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-08739-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091161851", 
          "https://doi.org/10.1038/s41598-017-08739-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-08739-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091161851", 
          "https://doi.org/10.1038/s41598-017-08739-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-08739-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091161851", 
          "https://doi.org/10.1038/s41598-017-08739-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fcell.2018.00008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100894002"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/ijo.2018.4390", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103777129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2217/imt-2017-0179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105018665"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2217/imt-2017-0179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105018665"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0205422", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1107500276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0205422", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1107500276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0205422", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1107500276"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-03-25", 
    "datePublishedReg": "2019-03-25", 
    "description": "The cilia and flagella of eukaryotic cells serve many functions, exhibiting remarkable conservation of both structure and molecular composition in widely divergent eukaryotic organisms. SPAG6 and SPAG16 are the homologous in the mice to Chlamydomonas reinhardtii PF16 and PF20. Both proteins are associated with the axonemal central apparatus and are essential for ciliary and flagellar motility in mammals. Recent data derived from high-throughput studies revealed expression of these genes in tissues that do not contain motile cilia. However, the distribution of SPAG6 and SPAG16 in ciliated and non-ciliated tissues is not completely understood. In this work, we performed a quantitative analysis of the expression of Spag6 and Spag16 genes in parallel with the immune-localization of the proteins in several tissues of adult mice. Expression of mRNA was higher in the testis and tissues bearing motile cilia than in the other analyzed tissues. Both proteins were present in ciliated and non-ciliated tissues. In the testis, SPAG6 was detected in spermatogonia, spermatocytes, and in the sperm flagella whereas SPAG16 was found in spermatocytes and in the sperm flagella. In addition, both proteins were detected in the cytoplasm of cells from the brain, spinal cord, and ovary. A small isoform of SPAG16 was localized in the nucleus of germ cells and some neurons. In a parallel set of experiments, we overexpressed EGFP-SPAG6 in cultured cells and observed that the protein co-localized with a subset of acetylated cytoplasmic microtubules. A role of these proteins stabilizing the cytoplasmic microtubules of eukaryotic cells is discussed.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10735-019-09817-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2564737", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1032970", 
        "issn": [
          "1567-2379", 
          "1573-6865"
        ], 
        "name": "Journal of Molecular Histology", 
        "type": "Periodical"
      }
    ], 
    "name": "Distribution of sperm antigen 6 (SPAG6) and 16 (SPAG16) in mouse ciliated and non-ciliated tissues", 
    "pagination": "1-14", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "54ce5f6a682375c004cf98c696ee16563763b0231578c55f06f484950f64a195"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30911868"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101193653"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10735-019-09817-z"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112985219"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10735-019-09817-z", 
      "https://app.dimensions.ai/details/publication/pub.1112985219"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000366_0000000366/records_112045_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs10735-019-09817-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10735-019-09817-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10735-019-09817-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10735-019-09817-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10735-019-09817-z'


 

This table displays all metadata directly associated to this object as RDF triples.

258 TRIPLES      21 PREDICATES      79 URIs      18 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10735-019-09817-z schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N5910e227bc45496c8e82836aa864dafc
4 schema:citation sg:pub.10.1007/s10735-016-9694-z
5 sg:pub.10.1038/356080a0
6 sg:pub.10.1038/nmeth.2019
7 sg:pub.10.1038/s41598-017-08739-8
8 sg:pub.10.1038/srep16506
9 sg:pub.10.1038/srep25840
10 sg:pub.10.1186/s13630-016-0030-8
11 sg:pub.10.1186/s13630-017-0046-8
12 https://app.dimensions.ai/details/publication/pub.1079207378
13 https://doi.org/10.1002/(sici)1097-0169(1997)38:1<1::aid-cm1>3.0.co;2-c
14 https://doi.org/10.1002/cm.20189
15 https://doi.org/10.1002/cm.21271
16 https://doi.org/10.1006/cbir.1996.0011
17 https://doi.org/10.1006/dbio.1999.9520
18 https://doi.org/10.1006/meth.2001.1262
19 https://doi.org/10.1016/0012-1606(75)90411-x
20 https://doi.org/10.1016/j.tcb.2005.04.008
21 https://doi.org/10.1016/j.tcb.2014.10.004
22 https://doi.org/10.1073/pnas.0404280101
23 https://doi.org/10.1073/pnas.0810777105
24 https://doi.org/10.1073/pnas.0906414106
25 https://doi.org/10.1074/jbc.m300688200
26 https://doi.org/10.1074/mcp.m400177-mcp200
27 https://doi.org/10.1083/jcb.100.6.2008
28 https://doi.org/10.1083/jcb.103.2.571
29 https://doi.org/10.1083/jcb.104.2.289
30 https://doi.org/10.1083/jcb.74.1.68
31 https://doi.org/10.1083/jcb.91.3.125s
32 https://doi.org/10.1093/molehr/gah163
33 https://doi.org/10.1095/biolreprod.105.049254
34 https://doi.org/10.1095/biolreprod.115.130989
35 https://doi.org/10.1095/biolreprod62.3.511
36 https://doi.org/10.1097/cji.0b013e3181fb64fa
37 https://doi.org/10.1111/j.1439-0264.2010.01000.x
38 https://doi.org/10.1128/mcb.22.17.6298-6305.2002
39 https://doi.org/10.1128/mcb.22.22.7993-8004.2002
40 https://doi.org/10.1146/annurev-pharmtox-011112-140326
41 https://doi.org/10.1146/annurev.physiol.69.040705.141236
42 https://doi.org/10.1158/1078-0432.ccr-05-2552
43 https://doi.org/10.1165/rcmb.2012-0362oc
44 https://doi.org/10.1242/jcs.050377
45 https://doi.org/10.1242/jcs.051326
46 https://doi.org/10.1371/journal.pcbi.1001065
47 https://doi.org/10.1371/journal.pone.0008189
48 https://doi.org/10.1371/journal.pone.0020625
49 https://doi.org/10.1371/journal.pone.0107271
50 https://doi.org/10.1371/journal.pone.0112879
51 https://doi.org/10.1371/journal.pone.0125936
52 https://doi.org/10.1371/journal.pone.0205422
53 https://doi.org/10.2217/imt-2017-0179
54 https://doi.org/10.3389/fcell.2018.00008
55 https://doi.org/10.3892/ijo.2014.2768
56 https://doi.org/10.3892/ijo.2018.4390
57 schema:datePublished 2019-03-25
58 schema:datePublishedReg 2019-03-25
59 schema:description The cilia and flagella of eukaryotic cells serve many functions, exhibiting remarkable conservation of both structure and molecular composition in widely divergent eukaryotic organisms. SPAG6 and SPAG16 are the homologous in the mice to Chlamydomonas reinhardtii PF16 and PF20. Both proteins are associated with the axonemal central apparatus and are essential for ciliary and flagellar motility in mammals. Recent data derived from high-throughput studies revealed expression of these genes in tissues that do not contain motile cilia. However, the distribution of SPAG6 and SPAG16 in ciliated and non-ciliated tissues is not completely understood. In this work, we performed a quantitative analysis of the expression of Spag6 and Spag16 genes in parallel with the immune-localization of the proteins in several tissues of adult mice. Expression of mRNA was higher in the testis and tissues bearing motile cilia than in the other analyzed tissues. Both proteins were present in ciliated and non-ciliated tissues. In the testis, SPAG6 was detected in spermatogonia, spermatocytes, and in the sperm flagella whereas SPAG16 was found in spermatocytes and in the sperm flagella. In addition, both proteins were detected in the cytoplasm of cells from the brain, spinal cord, and ovary. A small isoform of SPAG16 was localized in the nucleus of germ cells and some neurons. In a parallel set of experiments, we overexpressed EGFP-SPAG6 in cultured cells and observed that the protein co-localized with a subset of acetylated cytoplasmic microtubules. A role of these proteins stabilizing the cytoplasmic microtubules of eukaryotic cells is discussed.
60 schema:genre research_article
61 schema:inLanguage en
62 schema:isAccessibleForFree false
63 schema:isPartOf sg:journal.1032970
64 schema:name Distribution of sperm antigen 6 (SPAG6) and 16 (SPAG16) in mouse ciliated and non-ciliated tissues
65 schema:pagination 1-14
66 schema:productId N09cb948a5bbd41fba4c09b43bda91476
67 N1aaef52cf8924bb0b312656b354c6cdb
68 N23c910cbec6944128f89ae1b6f8621f5
69 N77ae2de5e1b6416983532a4896062a46
70 N86cc82dcd82e40cc83b266c10cb2766e
71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112985219
72 https://doi.org/10.1007/s10735-019-09817-z
73 schema:sdDatePublished 2019-04-11T13:04
74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
75 schema:sdPublisher N87845a112543418c9a764e7f5e5c9286
76 schema:url https://link.springer.com/10.1007%2Fs10735-019-09817-z
77 sgo:license sg:explorer/license/
78 sgo:sdDataset articles
79 rdf:type schema:ScholarlyArticle
80 N036bed620b5f4106b88ffaf8753c41f2 rdf:first N5445d280881d4ce2835959c92ad14ab8
81 rdf:rest Nebeb13e57d1a478e8b5cfe3154fb82c3
82 N09cb948a5bbd41fba4c09b43bda91476 schema:name pubmed_id
83 schema:value 30911868
84 rdf:type schema:PropertyValue
85 N1aaef52cf8924bb0b312656b354c6cdb schema:name dimensions_id
86 schema:value pub.1112985219
87 rdf:type schema:PropertyValue
88 N1ab75fb8b3af4d48b10d49adf3abedce schema:affiliation https://www.grid.ac/institutes/grid.11630.35
89 schema:familyName Anesetti
90 schema:givenName Gabriel
91 rdf:type schema:Person
92 N23c910cbec6944128f89ae1b6f8621f5 schema:name doi
93 schema:value 10.1007/s10735-019-09817-z
94 rdf:type schema:PropertyValue
95 N3f6242a5d38c4cbca4affd09db5e8df5 schema:affiliation https://www.grid.ac/institutes/grid.11630.35
96 schema:familyName Alciaturi
97 schema:givenName Jimena
98 rdf:type schema:Person
99 N5445d280881d4ce2835959c92ad14ab8 schema:affiliation https://www.grid.ac/institutes/grid.418532.9
100 schema:familyName Irigoin
101 schema:givenName Florencia
102 rdf:type schema:Person
103 N5910e227bc45496c8e82836aa864dafc rdf:first N3f6242a5d38c4cbca4affd09db5e8df5
104 rdf:rest N80af1ab48e3e4074a66083d55fc2945b
105 N60e5351020b54dbfa77dd27845c63112 rdf:first N6cf3d18541ea43099e72d4edf5876bba
106 rdf:rest rdf:nil
107 N6cf3d18541ea43099e72d4edf5876bba schema:affiliation https://www.grid.ac/institutes/grid.11630.35
108 schema:familyName Sapiro
109 schema:givenName Rossana
110 rdf:type schema:Person
111 N77ae2de5e1b6416983532a4896062a46 schema:name nlm_unique_id
112 schema:value 101193653
113 rdf:type schema:PropertyValue
114 N80af1ab48e3e4074a66083d55fc2945b rdf:first N1ab75fb8b3af4d48b10d49adf3abedce
115 rdf:rest N036bed620b5f4106b88ffaf8753c41f2
116 N86cc82dcd82e40cc83b266c10cb2766e schema:name readcube_id
117 schema:value 54ce5f6a682375c004cf98c696ee16563763b0231578c55f06f484950f64a195
118 rdf:type schema:PropertyValue
119 N87845a112543418c9a764e7f5e5c9286 schema:name Springer Nature - SN SciGraph project
120 rdf:type schema:Organization
121 Nebeb13e57d1a478e8b5cfe3154fb82c3 rdf:first Nf699691163fd4b328f09c7c318391582
122 rdf:rest N60e5351020b54dbfa77dd27845c63112
123 Nf699691163fd4b328f09c7c318391582 schema:affiliation https://www.grid.ac/institutes/grid.11630.35
124 schema:familyName Skowronek
125 schema:givenName Fernanda
126 rdf:type schema:Person
127 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
128 schema:name Biological Sciences
129 rdf:type schema:DefinedTerm
130 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
131 schema:name Biochemistry and Cell Biology
132 rdf:type schema:DefinedTerm
133 sg:grant.2564737 http://pending.schema.org/fundedItem sg:pub.10.1007/s10735-019-09817-z
134 rdf:type schema:MonetaryGrant
135 sg:journal.1032970 schema:issn 1567-2379
136 1573-6865
137 schema:name Journal of Molecular Histology
138 rdf:type schema:Periodical
139 sg:pub.10.1007/s10735-016-9694-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1029455653
140 https://doi.org/10.1007/s10735-016-9694-z
141 rdf:type schema:CreativeWork
142 sg:pub.10.1038/356080a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032052993
143 https://doi.org/10.1038/356080a0
144 rdf:type schema:CreativeWork
145 sg:pub.10.1038/nmeth.2019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026706357
146 https://doi.org/10.1038/nmeth.2019
147 rdf:type schema:CreativeWork
148 sg:pub.10.1038/s41598-017-08739-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091161851
149 https://doi.org/10.1038/s41598-017-08739-8
150 rdf:type schema:CreativeWork
151 sg:pub.10.1038/srep16506 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048660641
152 https://doi.org/10.1038/srep16506
153 rdf:type schema:CreativeWork
154 sg:pub.10.1038/srep25840 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010745449
155 https://doi.org/10.1038/srep25840
156 rdf:type schema:CreativeWork
157 sg:pub.10.1186/s13630-016-0030-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027428285
158 https://doi.org/10.1186/s13630-016-0030-8
159 rdf:type schema:CreativeWork
160 sg:pub.10.1186/s13630-017-0046-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084252548
161 https://doi.org/10.1186/s13630-017-0046-8
162 rdf:type schema:CreativeWork
163 https://app.dimensions.ai/details/publication/pub.1079207378 schema:CreativeWork
164 https://doi.org/10.1002/(sici)1097-0169(1997)38:1<1::aid-cm1>3.0.co;2-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1052943932
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1002/cm.20189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027412755
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1002/cm.21271 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007902797
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1006/cbir.1996.0011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013003822
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1006/dbio.1999.9520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011594986
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1006/meth.2001.1262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027621591
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1016/0012-1606(75)90411-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041951841
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/j.tcb.2005.04.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016257468
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/j.tcb.2014.10.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004236310
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1073/pnas.0404280101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021692115
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1073/pnas.0810777105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026670084
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1073/pnas.0906414106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028495935
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1074/jbc.m300688200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020795036
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1074/mcp.m400177-mcp200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043986155
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1083/jcb.100.6.2008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034426016
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1083/jcb.103.2.571 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046191493
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1083/jcb.104.2.289 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046168581
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1083/jcb.74.1.68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029870095
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1083/jcb.91.3.125s schema:sameAs https://app.dimensions.ai/details/publication/pub.1058438183
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1093/molehr/gah163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013882446
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1095/biolreprod.105.049254 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052243280
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1095/biolreprod.115.130989 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060111067
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1095/biolreprod62.3.511 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002240647
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1097/cji.0b013e3181fb64fa schema:sameAs https://app.dimensions.ai/details/publication/pub.1047705689
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1111/j.1439-0264.2010.01000.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1036162027
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1128/mcb.22.17.6298-6305.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004576014
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1128/mcb.22.22.7993-8004.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006134435
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1146/annurev-pharmtox-011112-140326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042629305
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1146/annurev.physiol.69.040705.141236 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025273003
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1158/1078-0432.ccr-05-2552 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041779391
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1165/rcmb.2012-0362oc schema:sameAs https://app.dimensions.ai/details/publication/pub.1015998056
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1242/jcs.050377 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023933181
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1242/jcs.051326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031173269
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1371/journal.pcbi.1001065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029034776
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1371/journal.pone.0008189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026311398
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1371/journal.pone.0020625 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029088213
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1371/journal.pone.0107271 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034806526
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1371/journal.pone.0112879 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031542095
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1371/journal.pone.0125936 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037521128
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1371/journal.pone.0205422 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107500276
243 rdf:type schema:CreativeWork
244 https://doi.org/10.2217/imt-2017-0179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105018665
245 rdf:type schema:CreativeWork
246 https://doi.org/10.3389/fcell.2018.00008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100894002
247 rdf:type schema:CreativeWork
248 https://doi.org/10.3892/ijo.2014.2768 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071513745
249 rdf:type schema:CreativeWork
250 https://doi.org/10.3892/ijo.2018.4390 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103777129
251 rdf:type schema:CreativeWork
252 https://www.grid.ac/institutes/grid.11630.35 schema:alternateName University of the Republic
253 schema:name Departamento de Histología y Embriología, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay
254 rdf:type schema:Organization
255 https://www.grid.ac/institutes/grid.418532.9 schema:alternateName Institut Pasteur de Montevideo
256 schema:name Departamento de Histología y Embriología, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay
257 Laboratorio de Genética Molecular Humana, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, Uruguay
258 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...