Localization of epithelial sodium channel (ENaC) and CFTR in the germinal epithelium of the testis, Sertoli cells, and spermatozoa View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-04

AUTHORS

Sachin Sharma, Aaron Hanukoglu, Israel Hanukoglu

ABSTRACT

Spermatogenesis starts within the seminiferous tubules of the testis by mitotic division of spermatogonia that produces spermatocytes. Meiotic division of these spermatocytes produces haploid spermatids that differentiate into spermatozoa. In this study, we examined the expression of ENaC and CFTR (a Cl- channel) in rat testicular sections using confocal microscopic immunofluorescence. The structural integrity of the seminiferous tubule sections was verified by precise phalloidin staining of the actin fibers located abundantly at both basal and adluminal tight junctions. The acrosome forming regions in the round spermatids were stained using an FITC coupled lectin (wheat germ agglutinin). In all phases of the germ cells (spermatogonia, spermatocytes, and spermatids) ENaC was localized in cytoplasmic pools. Prior to spermiation, ENaC immunofluorescence appeared along the tails of the spermatids. In spermatozoa isolated from the epididymis, ENaC was localized at the acrosome and a central region of the sperm flagellum. The mature sperm are transcriptionally silent. Hence, we suggest that ENaC subunits in cytoplasmic pools in germ cells serve as the source of ENaC subunits located along the tail of spermatozoa. The locations of ENaC is compatible with a possible role in the acrosomal reaction and sperm mobility. In contrast to ENaC, CFTR immunofluorescence was most strongly observed specifically within the Sertoli cell nuclei. Based on the nuclear localization of CFTR we suggest that, in addition to its role as an ion channel, CFTR may have an independent role in gene regulation within the nuclei. More... »

PAGES

195-208

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10735-018-9759-2

DOI

http://dx.doi.org/10.1007/s10735-018-9759-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1101078075

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29453757


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Acrosome", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blastodisc", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Nucleus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cystic Fibrosis Transmembrane Conductance Regulator", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epithelial Sodium Channels", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epithelium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Subunits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sertoli Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sperm Motility", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spermatozoa", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Testis", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Ariel University", 
          "id": "https://www.grid.ac/institutes/grid.411434.7", 
          "name": [
            "Laboratory of Cell Biology, Ariel University, 40700, Ariel, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sharma", 
        "givenName": "Sachin", 
        "id": "sg:person.016210203243.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016210203243.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wolfson Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.414317.4", 
          "name": [
            "Sackler Faculty of Medicine, Tel-Aviv University, Tel Aviv, Israel", 
            "Division of Pediatric Endocrinology, E. Wolfson Medical Center, Holon, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hanukoglu", 
        "givenName": "Aaron", 
        "id": "sg:person.0706304505.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706304505.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ariel University", 
          "id": "https://www.grid.ac/institutes/grid.411434.7", 
          "name": [
            "Laboratory of Cell Biology, Ariel University, 40700, Ariel, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hanukoglu", 
        "givenName": "Israel", 
        "id": "sg:person.01205074705.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205074705.88"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1749-6632.1952.tb26576.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000300818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajprenal.00116.2012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000827185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/aja.1001280302", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002131846"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajprenal.00648.2010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002159833"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2605.2008.00864.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008672638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.367606", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008718186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng0396-248", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009425431", 
          "https://doi.org/10.1038/ng0396-248"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2265.2005.02255.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010141752"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.semcdb.2014.02.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010758573"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-3476(06)80176-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015333614"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b924455g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016577257"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b924455g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016577257"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/andr.12165", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018337326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod63.1.99", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018456035"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod63.1.99", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018456035"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/febs.12765", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019208564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pmic.201600107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020370274"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/spmg.1.1.14525", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020768877"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-physiol-020911-153258", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021032277"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/febs.13840", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021465727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-319-31973-5_9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026316342", 
          "https://doi.org/10.1007/978-3-319-31973-5_9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.e04-11-1010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027718593"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.46.27411", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028385343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00232-010-9294-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028949967", 
          "https://doi.org/10.1007/s00232-010-9294-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00232-010-9294-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028949967", 
          "https://doi.org/10.1007/s00232-010-9294-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0104418", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029928484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsbmb.2008.06.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030835576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1756-0500-5-513", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033246958", 
          "https://doi.org/10.1186/1756-0500-5-513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajplung.00036.2012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034152636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humupd/dms027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034966230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrurol.2012.77", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036143365", 
          "https://doi.org/10.1038/nrurol.2012.77"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0022212", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036875724"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1046/j.1523-1755.2000.00968.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038431240", 
          "https://doi.org/10.1046/j.1523-1755.2000.00968.x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.gene.2015.12.061", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040150228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.gene.2015.12.061", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040150228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m508172200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044379796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00418-011-0904-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045398850", 
          "https://doi.org/10.1007/s00418-011-0904-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-319-30567-7_3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046660057", 
          "https://doi.org/10.1007/978-3-319-30567-7_3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.241245898", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047611423"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1530/rep-15-0463", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053428170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi060490t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055201919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi060490t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055201919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/aja.2010.62", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056439785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/aja.2010.62", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056439785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/er.2014-1101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064286086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/1573399811666150615144108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069206435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/spmg.26228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072310992"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1530/rep-16-0588", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074196692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00418-016-1535-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074242574", 
          "https://doi.org/10.1007/s00418-016-1535-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00418-016-1535-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074242574", 
          "https://doi.org/10.1007/s00418-016-1535-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpcell.1998.274.4.c922", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083256767"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.gene.2017.04.044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085074035"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-04", 
    "datePublishedReg": "2018-04-01", 
    "description": "Spermatogenesis starts within the seminiferous tubules of the testis by mitotic division of spermatogonia that produces spermatocytes. Meiotic division of these spermatocytes produces haploid spermatids that differentiate into spermatozoa. In this study, we examined the expression of ENaC and CFTR (a Cl- channel) in rat testicular sections using confocal microscopic immunofluorescence. The structural integrity of the seminiferous tubule sections was verified by precise phalloidin staining of the actin fibers located abundantly at both basal and adluminal tight junctions. The acrosome forming regions in the round spermatids were stained using an FITC coupled lectin (wheat germ agglutinin). In all phases of the germ cells (spermatogonia, spermatocytes, and spermatids) ENaC was localized in cytoplasmic pools. Prior to spermiation, ENaC immunofluorescence appeared along the tails of the spermatids. In spermatozoa isolated from the epididymis, ENaC was localized at the acrosome and a central region of the sperm flagellum. The mature sperm are transcriptionally silent. Hence, we suggest that ENaC subunits in cytoplasmic pools in germ cells serve as the source of ENaC subunits located along the tail of spermatozoa. The locations of ENaC is compatible with a possible role in the acrosomal reaction and sperm mobility. In contrast to ENaC, CFTR immunofluorescence was most strongly observed specifically within the Sertoli cell nuclei. Based on the nuclear localization of CFTR we suggest that, in addition to its role as an ion channel, CFTR may have an independent role in gene regulation within the nuclei.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10735-018-9759-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.7173253", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1032970", 
        "issn": [
          "1567-2379", 
          "1573-6865"
        ], 
        "name": "Journal of Molecular Histology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "49"
      }
    ], 
    "name": "Localization of epithelial sodium channel (ENaC) and CFTR in the germinal epithelium of the testis, Sertoli cells, and spermatozoa", 
    "pagination": "195-208", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "fc147783a38c412c55aa357a4ce655474c26e17ae819e0b1833c55ddde99d2c9"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29453757"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101193653"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10735-018-9759-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1101078075"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10735-018-9759-2", 
      "https://app.dimensions.ai/details/publication/pub.1101078075"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000502.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s10735-018-9759-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10735-018-9759-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10735-018-9759-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10735-018-9759-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10735-018-9759-2'


 

This table displays all metadata directly associated to this object as RDF triples.

289 TRIPLES      21 PREDICATES      88 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10735-018-9759-2 schema:about N0052656c26b04e3e94bff1645f6589cf
2 N04fb672e9dc34e0488cc09f390aeaf38
3 N0d75301e631449ee842bcbf644eb3a78
4 N19c69a2899374d0c91d769c5c768898d
5 N26094eaa70e143fe9c4490849fe4128d
6 N2b37cd06e11f422a9292364f3e55a8dd
7 N3bae6e893c244807ac3bf24695d6c6e2
8 N3e0764dc2ee2486684e2e608c424ba46
9 N536cdadc80764daa930180fd9bf582f1
10 N849f13ca75c3448eb81bd1e30b39f635
11 Nba086835a91a44c899c39f55d9e5bf9c
12 Nc9f542948a6b409eb90991fea31a3a55
13 Ncb5dccb9a6ce477e9a92992026256c16
14 Ne4a2bae04e594eef9d4377f3b6058d47
15 anzsrc-for:06
16 anzsrc-for:0601
17 schema:author N835d2acedbf24e1188abcc0ae20999fb
18 schema:citation sg:pub.10.1007/978-3-319-30567-7_3
19 sg:pub.10.1007/978-3-319-31973-5_9
20 sg:pub.10.1007/s00232-010-9294-x
21 sg:pub.10.1007/s00418-011-0904-1
22 sg:pub.10.1007/s00418-016-1535-3
23 sg:pub.10.1038/ng0396-248
24 sg:pub.10.1038/nrurol.2012.77
25 sg:pub.10.1046/j.1523-1755.2000.00968.x
26 sg:pub.10.1186/1756-0500-5-513
27 https://doi.org/10.1002/aja.1001280302
28 https://doi.org/10.1002/pmic.201600107
29 https://doi.org/10.1016/j.gene.2015.12.061
30 https://doi.org/10.1016/j.gene.2017.04.044
31 https://doi.org/10.1016/j.jsbmb.2008.06.013
32 https://doi.org/10.1016/j.semcdb.2014.02.008
33 https://doi.org/10.1016/s0022-3476(06)80176-9
34 https://doi.org/10.1021/bi060490t
35 https://doi.org/10.1038/aja.2010.62
36 https://doi.org/10.1039/b924455g
37 https://doi.org/10.1073/pnas.241245898
38 https://doi.org/10.1074/jbc.270.46.27411
39 https://doi.org/10.1074/jbc.m508172200
40 https://doi.org/10.1091/mbc.e04-11-1010
41 https://doi.org/10.1093/humupd/dms027
42 https://doi.org/10.1095/biolreprod63.1.99
43 https://doi.org/10.1101/gad.367606
44 https://doi.org/10.1111/andr.12165
45 https://doi.org/10.1111/febs.12765
46 https://doi.org/10.1111/febs.13840
47 https://doi.org/10.1111/j.1365-2265.2005.02255.x
48 https://doi.org/10.1111/j.1365-2605.2008.00864.x
49 https://doi.org/10.1111/j.1749-6632.1952.tb26576.x
50 https://doi.org/10.1146/annurev-physiol-020911-153258
51 https://doi.org/10.1152/ajpcell.1998.274.4.c922
52 https://doi.org/10.1152/ajplung.00036.2012
53 https://doi.org/10.1152/ajprenal.00116.2012
54 https://doi.org/10.1152/ajprenal.00648.2010
55 https://doi.org/10.1210/er.2014-1101
56 https://doi.org/10.1371/journal.pone.0022212
57 https://doi.org/10.1371/journal.pone.0104418
58 https://doi.org/10.1530/rep-15-0463
59 https://doi.org/10.1530/rep-16-0588
60 https://doi.org/10.2174/1573399811666150615144108
61 https://doi.org/10.4161/spmg.1.1.14525
62 https://doi.org/10.4161/spmg.26228
63 schema:datePublished 2018-04
64 schema:datePublishedReg 2018-04-01
65 schema:description Spermatogenesis starts within the seminiferous tubules of the testis by mitotic division of spermatogonia that produces spermatocytes. Meiotic division of these spermatocytes produces haploid spermatids that differentiate into spermatozoa. In this study, we examined the expression of ENaC and CFTR (a Cl<sup>-</sup> channel) in rat testicular sections using confocal microscopic immunofluorescence. The structural integrity of the seminiferous tubule sections was verified by precise phalloidin staining of the actin fibers located abundantly at both basal and adluminal tight junctions. The acrosome forming regions in the round spermatids were stained using an FITC coupled lectin (wheat germ agglutinin). In all phases of the germ cells (spermatogonia, spermatocytes, and spermatids) ENaC was localized in cytoplasmic pools. Prior to spermiation, ENaC immunofluorescence appeared along the tails of the spermatids. In spermatozoa isolated from the epididymis, ENaC was localized at the acrosome and a central region of the sperm flagellum. The mature sperm are transcriptionally silent. Hence, we suggest that ENaC subunits in cytoplasmic pools in germ cells serve as the source of ENaC subunits located along the tail of spermatozoa. The locations of ENaC is compatible with a possible role in the acrosomal reaction and sperm mobility. In contrast to ENaC, CFTR immunofluorescence was most strongly observed specifically within the Sertoli cell nuclei. Based on the nuclear localization of CFTR we suggest that, in addition to its role as an ion channel, CFTR may have an independent role in gene regulation within the nuclei.
66 schema:genre research_article
67 schema:inLanguage en
68 schema:isAccessibleForFree false
69 schema:isPartOf Nda48ec27ff0f4e4aabe8ca31197c96f1
70 Nf279cbb040554b289636ebf79838fe2a
71 sg:journal.1032970
72 schema:name Localization of epithelial sodium channel (ENaC) and CFTR in the germinal epithelium of the testis, Sertoli cells, and spermatozoa
73 schema:pagination 195-208
74 schema:productId N35b1479766694d269a89f177a244ed53
75 N7ae7db77cc02432bab6d782ed42d41d2
76 N7bbe06024bd2402bbcf950a204383207
77 N95d26cfac6b24eb99f7f6c7490b77c1f
78 Naecdceaeed5d482586e14f4be84354a5
79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101078075
80 https://doi.org/10.1007/s10735-018-9759-2
81 schema:sdDatePublished 2019-04-10T15:49
82 schema:sdLicense https://scigraph.springernature.com/explorer/license/
83 schema:sdPublisher N9173f5c75d554750b2e9397837d433f3
84 schema:url http://link.springer.com/10.1007/s10735-018-9759-2
85 sgo:license sg:explorer/license/
86 sgo:sdDataset articles
87 rdf:type schema:ScholarlyArticle
88 N0052656c26b04e3e94bff1645f6589cf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Cell Nucleus
90 rdf:type schema:DefinedTerm
91 N04fb672e9dc34e0488cc09f390aeaf38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Sertoli Cells
93 rdf:type schema:DefinedTerm
94 N0a8cbe2f13574b52b2318fa2fc03a8b7 rdf:first sg:person.01205074705.88
95 rdf:rest rdf:nil
96 N0d75301e631449ee842bcbf644eb3a78 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Epithelial Sodium Channels
98 rdf:type schema:DefinedTerm
99 N19c69a2899374d0c91d769c5c768898d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Acrosome
101 rdf:type schema:DefinedTerm
102 N26094eaa70e143fe9c4490849fe4128d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Sperm Motility
104 rdf:type schema:DefinedTerm
105 N2b37cd06e11f422a9292364f3e55a8dd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Blastodisc
107 rdf:type schema:DefinedTerm
108 N35b1479766694d269a89f177a244ed53 schema:name nlm_unique_id
109 schema:value 101193653
110 rdf:type schema:PropertyValue
111 N3bae6e893c244807ac3bf24695d6c6e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Rats
113 rdf:type schema:DefinedTerm
114 N3e0764dc2ee2486684e2e608c424ba46 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Male
116 rdf:type schema:DefinedTerm
117 N536cdadc80764daa930180fd9bf582f1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Animals
119 rdf:type schema:DefinedTerm
120 N73099febabb94ffda461802427bd31dc rdf:first sg:person.0706304505.05
121 rdf:rest N0a8cbe2f13574b52b2318fa2fc03a8b7
122 N7ae7db77cc02432bab6d782ed42d41d2 schema:name readcube_id
123 schema:value fc147783a38c412c55aa357a4ce655474c26e17ae819e0b1833c55ddde99d2c9
124 rdf:type schema:PropertyValue
125 N7bbe06024bd2402bbcf950a204383207 schema:name doi
126 schema:value 10.1007/s10735-018-9759-2
127 rdf:type schema:PropertyValue
128 N835d2acedbf24e1188abcc0ae20999fb rdf:first sg:person.016210203243.47
129 rdf:rest N73099febabb94ffda461802427bd31dc
130 N849f13ca75c3448eb81bd1e30b39f635 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Epithelium
132 rdf:type schema:DefinedTerm
133 N9173f5c75d554750b2e9397837d433f3 schema:name Springer Nature - SN SciGraph project
134 rdf:type schema:Organization
135 N95d26cfac6b24eb99f7f6c7490b77c1f schema:name dimensions_id
136 schema:value pub.1101078075
137 rdf:type schema:PropertyValue
138 Naecdceaeed5d482586e14f4be84354a5 schema:name pubmed_id
139 schema:value 29453757
140 rdf:type schema:PropertyValue
141 Nba086835a91a44c899c39f55d9e5bf9c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Spermatozoa
143 rdf:type schema:DefinedTerm
144 Nc9f542948a6b409eb90991fea31a3a55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Testis
146 rdf:type schema:DefinedTerm
147 Ncb5dccb9a6ce477e9a92992026256c16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Protein Subunits
149 rdf:type schema:DefinedTerm
150 Nda48ec27ff0f4e4aabe8ca31197c96f1 schema:issueNumber 2
151 rdf:type schema:PublicationIssue
152 Ne4a2bae04e594eef9d4377f3b6058d47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Cystic Fibrosis Transmembrane Conductance Regulator
154 rdf:type schema:DefinedTerm
155 Nf279cbb040554b289636ebf79838fe2a schema:volumeNumber 49
156 rdf:type schema:PublicationVolume
157 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
158 schema:name Biological Sciences
159 rdf:type schema:DefinedTerm
160 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
161 schema:name Biochemistry and Cell Biology
162 rdf:type schema:DefinedTerm
163 sg:grant.7173253 http://pending.schema.org/fundedItem sg:pub.10.1007/s10735-018-9759-2
164 rdf:type schema:MonetaryGrant
165 sg:journal.1032970 schema:issn 1567-2379
166 1573-6865
167 schema:name Journal of Molecular Histology
168 rdf:type schema:Periodical
169 sg:person.01205074705.88 schema:affiliation https://www.grid.ac/institutes/grid.411434.7
170 schema:familyName Hanukoglu
171 schema:givenName Israel
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205074705.88
173 rdf:type schema:Person
174 sg:person.016210203243.47 schema:affiliation https://www.grid.ac/institutes/grid.411434.7
175 schema:familyName Sharma
176 schema:givenName Sachin
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016210203243.47
178 rdf:type schema:Person
179 sg:person.0706304505.05 schema:affiliation https://www.grid.ac/institutes/grid.414317.4
180 schema:familyName Hanukoglu
181 schema:givenName Aaron
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706304505.05
183 rdf:type schema:Person
184 sg:pub.10.1007/978-3-319-30567-7_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046660057
185 https://doi.org/10.1007/978-3-319-30567-7_3
186 rdf:type schema:CreativeWork
187 sg:pub.10.1007/978-3-319-31973-5_9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026316342
188 https://doi.org/10.1007/978-3-319-31973-5_9
189 rdf:type schema:CreativeWork
190 sg:pub.10.1007/s00232-010-9294-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028949967
191 https://doi.org/10.1007/s00232-010-9294-x
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/s00418-011-0904-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045398850
194 https://doi.org/10.1007/s00418-011-0904-1
195 rdf:type schema:CreativeWork
196 sg:pub.10.1007/s00418-016-1535-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074242574
197 https://doi.org/10.1007/s00418-016-1535-3
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/ng0396-248 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009425431
200 https://doi.org/10.1038/ng0396-248
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/nrurol.2012.77 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036143365
203 https://doi.org/10.1038/nrurol.2012.77
204 rdf:type schema:CreativeWork
205 sg:pub.10.1046/j.1523-1755.2000.00968.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038431240
206 https://doi.org/10.1046/j.1523-1755.2000.00968.x
207 rdf:type schema:CreativeWork
208 sg:pub.10.1186/1756-0500-5-513 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033246958
209 https://doi.org/10.1186/1756-0500-5-513
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1002/aja.1001280302 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002131846
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1002/pmic.201600107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020370274
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/j.gene.2015.12.061 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040150228
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/j.gene.2017.04.044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085074035
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/j.jsbmb.2008.06.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030835576
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/j.semcdb.2014.02.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010758573
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/s0022-3476(06)80176-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015333614
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1021/bi060490t schema:sameAs https://app.dimensions.ai/details/publication/pub.1055201919
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1038/aja.2010.62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056439785
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1039/b924455g schema:sameAs https://app.dimensions.ai/details/publication/pub.1016577257
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1073/pnas.241245898 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047611423
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1074/jbc.270.46.27411 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028385343
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1074/jbc.m508172200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044379796
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1091/mbc.e04-11-1010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027718593
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1093/humupd/dms027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034966230
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1095/biolreprod63.1.99 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018456035
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1101/gad.367606 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008718186
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1111/andr.12165 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018337326
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1111/febs.12765 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019208564
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1111/febs.13840 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021465727
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1111/j.1365-2265.2005.02255.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010141752
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1111/j.1365-2605.2008.00864.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008672638
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1111/j.1749-6632.1952.tb26576.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1000300818
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1146/annurev-physiol-020911-153258 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021032277
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1152/ajpcell.1998.274.4.c922 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083256767
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1152/ajplung.00036.2012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034152636
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1152/ajprenal.00116.2012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000827185
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1152/ajprenal.00648.2010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002159833
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1210/er.2014-1101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064286086
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1371/journal.pone.0022212 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036875724
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1371/journal.pone.0104418 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029928484
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1530/rep-15-0463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053428170
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1530/rep-16-0588 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074196692
276 rdf:type schema:CreativeWork
277 https://doi.org/10.2174/1573399811666150615144108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069206435
278 rdf:type schema:CreativeWork
279 https://doi.org/10.4161/spmg.1.1.14525 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020768877
280 rdf:type schema:CreativeWork
281 https://doi.org/10.4161/spmg.26228 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072310992
282 rdf:type schema:CreativeWork
283 https://www.grid.ac/institutes/grid.411434.7 schema:alternateName Ariel University
284 schema:name Laboratory of Cell Biology, Ariel University, 40700, Ariel, Israel
285 rdf:type schema:Organization
286 https://www.grid.ac/institutes/grid.414317.4 schema:alternateName Wolfson Medical Center
287 schema:name Division of Pediatric Endocrinology, E. Wolfson Medical Center, Holon, Israel
288 Sackler Faculty of Medicine, Tel-Aviv University, Tel Aviv, Israel
289 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...