Rapid micropropagation system in vitro and antioxidant activity of Scabiosa tschiliensis Grunning View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-10-30

AUTHORS

Junli Wang, Kun Liu, Dongting Xu, Qian Wang, Kaili Bi, Yunfei Song, Jianfei Li, Lu Zhang

ABSTRACT

A rapid micropropagation system for Scabiosa tschiliensis Grunning, an ethnic medicinal plant, has been developed. Calluses were induced from leaf and petiole explants on Murashige and Skoog (MS) medium supplemented with 2.0 mg l−1 thidiazuron and 0.5 mg l−1 2,4-dicholorophenoxyacetic acid. In this medium, callus induction rate was about 94.05 %. Adventitious shoots developed from leaf (86.30 %) and petiole (83.33 %) calluses when cultured on MS medium containing 4.0 or 2.0 mg l−1 N6-benzyladenine (BA), respectively. Up to 73.85 % of the regenerated shoots formed complete plantlets on MS medium supplemented with 2.0 mg l−1 indole-3-butyric acid, with an average of 3.25 roots per shoot. Quantitative analysis of flavonoids showed that the phytochemical profiles of calluses and regenerated plants were similar to that of wild-type plants. The 2, 2-diphenyl-1-picrylhydrazyl assay revealed that the flavonoid extracts of calluses, adventitious shoots and wild-type plants had stronger antioxidant activities, the inhibitory concentrations being 23.944, 31.329 and 26.502 μg ml−1, respectively, where 50 % of DPPH was scavenged (IC50). Results showed that this perennial herb could be used as a potential source of new natural antioxidants. More... »

PAGES

305-310

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10725-012-9765-4

DOI

http://dx.doi.org/10.1007/s10725-012-9765-4

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https://app.dimensions.ai/details/publication/pub.1050403104


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