Separation of glycosphingolipids with titanium dioxide View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-10-04

AUTHORS

Ayaka Noda, Miki Kato, Shota Miyazaki, Mamoru Kyogashima

ABSTRACT

We introduce the principle of a new technique to isolate glycosphingolipids (GSLs) from phospholipids. Neutral and acidic GSLs in organic solvent bind to titanium dioxide under neutral pH and can be eluted with 5 mg/ml of 2,5-dihydroxybenzoic acid in methanol. This special property is applicable for eliminating phospholipids, including sphingomyelin, which cannot be eliminated by a typical mild alkaline treatment. By using this technique, we demonstrated the rapid separation of minor components of GSLs, namely sulfatide and gangliosides from rabbit serum and liver, respectively. The minor GSL components were effectively purified despite both sources containing tremendous amount of phospholipids and simple lipids such as cholesterol, cholesteryl esters and triglycerides. More... »

PAGES

493-498

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10719-018-9844-5

DOI

http://dx.doi.org/10.1007/s10719-018-9844-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1107397294

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30284662


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