Structure and biological activities of a hexosamine-rich cell wall polysaccharide isolated from the probiotic Lactobacillus farciminis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-01-12

AUTHORS

Emmanuel Maes, Irina Sadovskaya, Mathilde Lévêque, Elisabeth Elass-Rochard, Bruno Payré, Thierry Grard, Vassilia Théodorou, Yann Guérardel, Muriel Mercier-Bonin

ABSTRACT

Lactobacillus farciminis CIP 103136 is a bacterial strain with recognized probiotic properties. However, the mechanisms underlying such properties have only been partially elucidated. In this study, we isolated and purified a cell-wall associated polysaccharide (CWPS), and evaluated its biological role in vitro. The structure of CWPS and responses from stimulation of (i) human macrophage-like THP-1 cells, (ii) human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR2 or TLR4) and (iii) human colonocyte-like T84 intestinal epithelial cells, upon exposure to CWPS were studied. The structure of the purified CWPS from L. farciminis CIP 103136 was analyzed by nuclear magnetic resonance (NMR), MALDI-TOF-TOF MS, and methylation analyses in its native form and following Smith degradation. It was shown to be a novel branched polysaccharide, composed of linear backbone of trisaccharide repeating units of: [→6αGlcpNAc1 → 4βManpNAc1 → 4βGlcpNAc1→] highly substituted with single residues of αGlcp, αGalp and αGlcpNAc. Subsequently, the lack of pro- or anti-inflammatory properties of CWPS was established on macrophage-like THP-1 cells. In addition, CWPS failed to modulate cell signaling pathways dependent of TLR2 and TLR4 in transfected HEK-cells. Finally, in T84 cells, CWPS neither influenced intestinal barrier integrity under basal conditions nor prevented TNF-α/IFN-γ cytokine-mediated epithelium impairment. More... »

PAGES

39-55

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10719-018-09854-y

DOI

http://dx.doi.org/10.1007/s10719-018-09854-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111375189

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30637506


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85 polysaccharides
86 probiotic Lactobacillus farciminis
87 probiotic properties
88 properties
89 receptors
90 residues
91 resonance
92 response
93 role
94 single residue
95 stimulation
96 strains
97 structure
98 structure of CWPS
99 study
100 such properties
101 trisaccharide
102 units
103 wall polysaccharides
104 αGalp
105 αGlcp
106 αGlcpNAc
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