Building a PGC-LC-MS N-glycan retention library and elution mapping resource View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-09-13

AUTHORS

Jodie L. Abrahams, Matthew P. Campbell, Nicolle H. Packer

ABSTRACT

Porous graphitised carbon-liquid chromatography (PGC-LC) has been proven to be a powerful technique for the analysis and characterisation of complex mixtures of isomeric and isobaric glycan structures. Here we evaluate the elution behaviour of N-glycans on PGC-LC and thereby provide the potential of using chromatographic separation properties, together with mass spectrometry (MS) fragmentation, to determine glycan structure assignments more easily. We used previously reported N-glycan structures released from the purified glycoproteins Immunoglobulin G (IgG), Immunoglobulin A (IgA), lactoferrin, α1-acid glycoprotein, Ribonuclease B (RNase B), fetuin and ovalbumin to profile their behaviour on capillary PGC-LC-MS. Over 100 glycan structures were determined by MS/MS, and together with targeted exoglycosidase digestions, created a N-glycan PGC retention library covering a full spectrum of biologically significant N-glycans from pauci mannose to sialylated tetra-antennary classes. The resultant PGC retention library (http://www.glycostore.org/showPgc) incorporates retention times and supporting fragmentation spectra including exoglycosidase digestion products, and provides detailed knowledge on the elution properties of N-glycans by PGC-LC. Consequently, this platform should serve as a valuable resource for facilitating the detailed analysis of the glycosylation of both purified recombinant, and complex mixtures of, glycoproteins using established workflows. More... »

PAGES

15-29

References to SciGraph publications

  • 2005-05-01. Fragmentation of negative ions from carbohydrates: Part 3. Fragmentation of hybrid and complex N-linked glycans in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2005-05-01. Fragmentation of negative ions from carbohydrates: Part 2. Fragmentation of high-mannose N-linked glycans in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 1998-02-27. Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM) in GLYCOANALYSIS PROTOCOLS
  • 2011-05-31. Genetics and the environment converge to dysregulate N-glycosylation in multiple sclerosis in NATURE COMMUNICATIONS
  • 2012-04-25. Glycomics using mass spectrometry in GLYCOCONJUGATE JOURNAL
  • 2008-11-27. The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2014-01-10. Toolboxes for a standardised and systematic study of glycans in BMC BIOINFORMATICS
  • 2013-01-31. Reversed-phase liquid-chromatographic mass spectrometric N-glycan analysis of biopharmaceuticals in ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • 2000-06-01. Composition of N-linked carbohydrates from ovalbumin and co-purified glycoproteins in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2014-12-09. Clinical Glycomics Employing Graphitized Carbon Liquid Chromatography–Mass Spectrometry in CHROMATOGRAPHIA
  • 2015. Isomer-Specific Analysis of Released N-Glycans by LC-ESI MS/MS with Porous Graphitized Carbon in GLYCO-ENGINEERING
  • 2009-02-27. Oligosaccharide analysis by graphitized carbon liquid chromatography–mass spectrometry in ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • 2006-09-15. Detailed Structural Analysis of N-Glycans Released From Glycoproteins in SDS-PAGE Gel Bands Using HPLC Combined With Exoglycosidase Array Digestions in GLYCOBIOLOGY PROTOCOLS
  • 2013-04-20. Structural Feature Ions for Distinguishing N- and O-Linked Glycan Isomers by LC-ESI-IT MS/MS in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2012-06-07. Structural analysis of N- and O-glycans released from glycoproteins in NATURE PROTOCOLS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10719-017-9793-4

    DOI

    http://dx.doi.org/10.1007/s10719-017-9793-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1091593561

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28905148


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Neurosciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromatography, Liquid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Glycoproteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Graphite", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mass Spectrometry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polysaccharides", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Institute for Glycomics, Griffith University, QLD, 4222, Gold Coast, Australia", 
              "id": "http://www.grid.ac/institutes/grid.1022.1", 
              "name": [
                "Department of Chemistry and Biomolecular Sciences, Faculty of Science & Engineering, Macquarie University, North Ryde, 2109, Sydney, NSW, Australia", 
                "Institute for Glycomics, Griffith University, QLD, 4222, Gold Coast, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Abrahams", 
            "givenName": "Jodie L.", 
            "id": "sg:person.0732346463.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732346463.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute for Glycomics, Griffith University, QLD, 4222, Gold Coast, Australia", 
              "id": "http://www.grid.ac/institutes/grid.1022.1", 
              "name": [
                "Department of Chemistry and Biomolecular Sciences, Faculty of Science & Engineering, Macquarie University, North Ryde, 2109, Sydney, NSW, Australia", 
                "Institute for Glycomics, Griffith University, QLD, 4222, Gold Coast, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Campbell", 
            "givenName": "Matthew P.", 
            "id": "sg:person.01303007173.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303007173.10"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute for Glycomics, Griffith University, QLD, 4222, Gold Coast, Australia", 
              "id": "http://www.grid.ac/institutes/grid.1022.1", 
              "name": [
                "Department of Chemistry and Biomolecular Sciences, Faculty of Science & Engineering, Macquarie University, North Ryde, 2109, Sydney, NSW, Australia", 
                "Institute for Glycomics, Griffith University, QLD, 4222, Gold Coast, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Packer", 
            "givenName": "Nicolle H.", 
            "id": "sg:person.0657131714.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657131714.23"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s00216-009-2664-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045598050", 
              "https://doi.org/10.1007/s00216-009-2664-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1016/s1044-0305(00)00122-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016033386", 
              "https://doi.org/10.1016/s1044-0305(00)00122-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1385/1-59745-167-3:125", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000245844", 
              "https://doi.org/10.1385/1-59745-167-3:125"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms1333", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018591287", 
              "https://doi.org/10.1038/ncomms1333"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2012.063", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035692290", 
              "https://doi.org/10.1038/nprot.2012.063"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1385/0-89603-355-4:53", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083291092", 
              "https://doi.org/10.1385/0-89603-355-4:53"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10719-012-9376-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019594761", 
              "https://doi.org/10.1007/s10719-012-9376-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1016/j.jasms.2008.11.012", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029202640", 
              "https://doi.org/10.1016/j.jasms.2008.11.012"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1016/j.jasms.2005.01.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012784675", 
              "https://doi.org/10.1016/j.jasms.2005.01.005"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4939-2760-9_29", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041260983", 
              "https://doi.org/10.1007/978-1-4939-2760-9_29"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10337-014-2813-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019339575", 
              "https://doi.org/10.1007/s10337-014-2813-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2105-15-s1-s9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018559057", 
              "https://doi.org/10.1186/1471-2105-15-s1-s9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s13361-013-0610-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004464648", 
              "https://doi.org/10.1007/s13361-013-0610-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1016/j.jasms.2005.01.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027892668", 
              "https://doi.org/10.1016/j.jasms.2005.01.006"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00216-012-6690-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020197428", 
              "https://doi.org/10.1007/s00216-012-6690-3"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017-09-13", 
        "datePublishedReg": "2017-09-13", 
        "description": "Porous graphitised carbon-liquid chromatography (PGC-LC) has been proven to be a powerful technique for the analysis and characterisation of complex mixtures of isomeric and isobaric glycan structures. Here we evaluate the elution behaviour of N-glycans on PGC-LC and thereby provide the potential of using chromatographic separation properties, together with mass spectrometry  (MS) fragmentation, to determine glycan structure assignments more easily. We used previously reported N-glycan structures released from the purified glycoproteins Immunoglobulin G (IgG), Immunoglobulin A (IgA), lactoferrin, \u03b11-acid glycoprotein, Ribonuclease B (RNase B), fetuin and ovalbumin to profile their behaviour on capillary PGC-LC-MS. Over 100 glycan structures were determined by MS/MS, and together with targeted exoglycosidase digestions, created a N-glycan PGC retention library covering a full spectrum of biologically significant N-glycans from pauci mannose to sialylated tetra-antennary classes. The resultant PGC retention library (http://www.glycostore.org/showPgc) incorporates retention times and supporting fragmentation spectra including exoglycosidase digestion products, and provides detailed knowledge on the elution properties of N-glycans by PGC-LC. Consequently, this platform should serve as a valuable resource for facilitating the detailed analysis of the glycosylation of both purified recombinant, and complex mixtures of, glycoproteins using established workflows.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s10719-017-9793-4", 
        "inLanguage": "en", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1096094", 
            "issn": [
              "0969-3653", 
              "1573-4986"
            ], 
            "name": "Glycoconjugate Journal", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "35"
          }
        ], 
        "keywords": [
          "chromatographic separation properties", 
          "complex mixtures", 
          "isobaric glycan structures", 
          "glycan structures", 
          "MS/MS", 
          "separation properties", 
          "elution behavior", 
          "ribonuclease B", 
          "fragmentation spectra", 
          "glycoprotein immunoglobulin G", 
          "MS n", 
          "elution properties", 
          "retention time", 
          "powerful technique", 
          "mixture", 
          "MS", 
          "spectra", 
          "structure", 
          "\u03b11-acid glycoprotein", 
          "properties", 
          "digestion products", 
          "glycans", 
          "exoglycosidase digestion", 
          "chromatography", 
          "characterisation", 
          "products", 
          "detailed knowledge", 
          "behavior", 
          "detailed analysis", 
          "immunoglobulin G", 
          "mannose", 
          "potential", 
          "fragmentation", 
          "glycosylation", 
          "technique", 
          "analysis", 
          "fetuin", 
          "library", 
          "digestion", 
          "glycoprotein", 
          "platform", 
          "lactoferrin", 
          "class", 
          "time", 
          "mass", 
          "full spectrum", 
          "workflow", 
          "valuable resource", 
          "knowledge", 
          "resources", 
          "immunoglobulin A", 
          "mapping resources", 
          "PGC-LC", 
          "graphitised carbon-liquid chromatography", 
          "carbon-liquid chromatography", 
          "capillary PGC-LC", 
          "targeted exoglycosidase digestions", 
          "N-glycan PGC retention library", 
          "PGC retention library", 
          "retention library", 
          "pauci mannose", 
          "tetra-antennary classes", 
          "resultant PGC retention library", 
          "exoglycosidase digestion products", 
          "glycan retention library", 
          "elution mapping resource"
        ], 
        "name": "Building a PGC-LC-MS N-glycan retention library and elution mapping resource", 
        "pagination": "15-29", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1091593561"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s10719-017-9793-4"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "28905148"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s10719-017-9793-4", 
          "https://app.dimensions.ai/details/publication/pub.1091593561"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2021-11-01T18:28", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_727.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s10719-017-9793-4"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10719-017-9793-4'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10719-017-9793-4'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10719-017-9793-4'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10719-017-9793-4'


     

    This table displays all metadata directly associated to this object as RDF triples.

    235 TRIPLES      22 PREDICATES      115 URIs      89 LITERALS      12 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s10719-017-9793-4 schema:about N174cd0153fc145cf9a2acf93e8e1cdad
    2 N1848a633c6bd4719922c48875f91350e
    3 N2d360d70cbcc4f50a34057444d26cebb
    4 N4abc7e0db0b549dc91645f6b421a1074
    5 Ne02de1061b574048bb79dc4e460fa122
    6 anzsrc-for:06
    7 anzsrc-for:0601
    8 anzsrc-for:11
    9 anzsrc-for:1108
    10 anzsrc-for:1109
    11 schema:author N795892b3a9ae4bcaa8418619d22ddf77
    12 schema:citation sg:pub.10.1007/978-1-4939-2760-9_29
    13 sg:pub.10.1007/s00216-009-2664-5
    14 sg:pub.10.1007/s00216-012-6690-3
    15 sg:pub.10.1007/s10337-014-2813-7
    16 sg:pub.10.1007/s10719-012-9376-3
    17 sg:pub.10.1007/s13361-013-0610-4
    18 sg:pub.10.1016/j.jasms.2005.01.005
    19 sg:pub.10.1016/j.jasms.2005.01.006
    20 sg:pub.10.1016/j.jasms.2008.11.012
    21 sg:pub.10.1016/s1044-0305(00)00122-7
    22 sg:pub.10.1038/ncomms1333
    23 sg:pub.10.1038/nprot.2012.063
    24 sg:pub.10.1186/1471-2105-15-s1-s9
    25 sg:pub.10.1385/0-89603-355-4:53
    26 sg:pub.10.1385/1-59745-167-3:125
    27 schema:datePublished 2017-09-13
    28 schema:datePublishedReg 2017-09-13
    29 schema:description Porous graphitised carbon-liquid chromatography (PGC-LC) has been proven to be a powerful technique for the analysis and characterisation of complex mixtures of isomeric and isobaric glycan structures. Here we evaluate the elution behaviour of N-glycans on PGC-LC and thereby provide the potential of using chromatographic separation properties, together with mass spectrometry (MS) fragmentation, to determine glycan structure assignments more easily. We used previously reported N-glycan structures released from the purified glycoproteins Immunoglobulin G (IgG), Immunoglobulin A (IgA), lactoferrin, α1-acid glycoprotein, Ribonuclease B (RNase B), fetuin and ovalbumin to profile their behaviour on capillary PGC-LC-MS. Over 100 glycan structures were determined by MS/MS, and together with targeted exoglycosidase digestions, created a N-glycan PGC retention library covering a full spectrum of biologically significant N-glycans from pauci mannose to sialylated tetra-antennary classes. The resultant PGC retention library (http://www.glycostore.org/showPgc) incorporates retention times and supporting fragmentation spectra including exoglycosidase digestion products, and provides detailed knowledge on the elution properties of N-glycans by PGC-LC. Consequently, this platform should serve as a valuable resource for facilitating the detailed analysis of the glycosylation of both purified recombinant, and complex mixtures of, glycoproteins using established workflows.
    30 schema:genre article
    31 schema:inLanguage en
    32 schema:isAccessibleForFree false
    33 schema:isPartOf Nbbf68772c3424c909cb098d39ea89b25
    34 Nbe9ad7c3a2cb47398515c0f6274342fc
    35 sg:journal.1096094
    36 schema:keywords MS
    37 MS n
    38 MS/MS
    39 N-glycan PGC retention library
    40 PGC retention library
    41 PGC-LC
    42 analysis
    43 behavior
    44 capillary PGC-LC
    45 carbon-liquid chromatography
    46 characterisation
    47 chromatographic separation properties
    48 chromatography
    49 class
    50 complex mixtures
    51 detailed analysis
    52 detailed knowledge
    53 digestion
    54 digestion products
    55 elution behavior
    56 elution mapping resource
    57 elution properties
    58 exoglycosidase digestion
    59 exoglycosidase digestion products
    60 fetuin
    61 fragmentation
    62 fragmentation spectra
    63 full spectrum
    64 glycan retention library
    65 glycan structures
    66 glycans
    67 glycoprotein
    68 glycoprotein immunoglobulin G
    69 glycosylation
    70 graphitised carbon-liquid chromatography
    71 immunoglobulin A
    72 immunoglobulin G
    73 isobaric glycan structures
    74 knowledge
    75 lactoferrin
    76 library
    77 mannose
    78 mapping resources
    79 mass
    80 mixture
    81 pauci mannose
    82 platform
    83 potential
    84 powerful technique
    85 products
    86 properties
    87 resources
    88 resultant PGC retention library
    89 retention library
    90 retention time
    91 ribonuclease B
    92 separation properties
    93 spectra
    94 structure
    95 targeted exoglycosidase digestions
    96 technique
    97 tetra-antennary classes
    98 time
    99 valuable resource
    100 workflow
    101 α1-acid glycoprotein
    102 schema:name Building a PGC-LC-MS N-glycan retention library and elution mapping resource
    103 schema:pagination 15-29
    104 schema:productId N266facbb69a24d0aa9e070d0b8a8b262
    105 N63895cfbf1d34104b662d36b61bcb5b8
    106 N8ed60bd25b5444f08c54aea5890c697d
    107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091593561
    108 https://doi.org/10.1007/s10719-017-9793-4
    109 schema:sdDatePublished 2021-11-01T18:28
    110 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    111 schema:sdPublisher N748e71d7510f4dd1a7b1a5251156745d
    112 schema:url https://doi.org/10.1007/s10719-017-9793-4
    113 sgo:license sg:explorer/license/
    114 sgo:sdDataset articles
    115 rdf:type schema:ScholarlyArticle
    116 N174cd0153fc145cf9a2acf93e8e1cdad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Chromatography, Liquid
    118 rdf:type schema:DefinedTerm
    119 N1848a633c6bd4719922c48875f91350e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Graphite
    121 rdf:type schema:DefinedTerm
    122 N266facbb69a24d0aa9e070d0b8a8b262 schema:name doi
    123 schema:value 10.1007/s10719-017-9793-4
    124 rdf:type schema:PropertyValue
    125 N2d360d70cbcc4f50a34057444d26cebb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Polysaccharides
    127 rdf:type schema:DefinedTerm
    128 N4abc7e0db0b549dc91645f6b421a1074 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Mass Spectrometry
    130 rdf:type schema:DefinedTerm
    131 N59d34de5ced94eec92c4c34953acef72 rdf:first sg:person.01303007173.10
    132 rdf:rest N91950b798e464347989e609bd0b823bf
    133 N63895cfbf1d34104b662d36b61bcb5b8 schema:name dimensions_id
    134 schema:value pub.1091593561
    135 rdf:type schema:PropertyValue
    136 N748e71d7510f4dd1a7b1a5251156745d schema:name Springer Nature - SN SciGraph project
    137 rdf:type schema:Organization
    138 N795892b3a9ae4bcaa8418619d22ddf77 rdf:first sg:person.0732346463.23
    139 rdf:rest N59d34de5ced94eec92c4c34953acef72
    140 N8ed60bd25b5444f08c54aea5890c697d schema:name pubmed_id
    141 schema:value 28905148
    142 rdf:type schema:PropertyValue
    143 N91950b798e464347989e609bd0b823bf rdf:first sg:person.0657131714.23
    144 rdf:rest rdf:nil
    145 Nbbf68772c3424c909cb098d39ea89b25 schema:issueNumber 1
    146 rdf:type schema:PublicationIssue
    147 Nbe9ad7c3a2cb47398515c0f6274342fc schema:volumeNumber 35
    148 rdf:type schema:PublicationVolume
    149 Ne02de1061b574048bb79dc4e460fa122 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Glycoproteins
    151 rdf:type schema:DefinedTerm
    152 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    153 schema:name Biological Sciences
    154 rdf:type schema:DefinedTerm
    155 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    156 schema:name Biochemistry and Cell Biology
    157 rdf:type schema:DefinedTerm
    158 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    159 schema:name Medical and Health Sciences
    160 rdf:type schema:DefinedTerm
    161 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
    162 schema:name Medical Microbiology
    163 rdf:type schema:DefinedTerm
    164 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
    165 schema:name Neurosciences
    166 rdf:type schema:DefinedTerm
    167 sg:journal.1096094 schema:issn 0969-3653
    168 1573-4986
    169 schema:name Glycoconjugate Journal
    170 schema:publisher Springer Nature
    171 rdf:type schema:Periodical
    172 sg:person.01303007173.10 schema:affiliation grid-institutes:grid.1022.1
    173 schema:familyName Campbell
    174 schema:givenName Matthew P.
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303007173.10
    176 rdf:type schema:Person
    177 sg:person.0657131714.23 schema:affiliation grid-institutes:grid.1022.1
    178 schema:familyName Packer
    179 schema:givenName Nicolle H.
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657131714.23
    181 rdf:type schema:Person
    182 sg:person.0732346463.23 schema:affiliation grid-institutes:grid.1022.1
    183 schema:familyName Abrahams
    184 schema:givenName Jodie L.
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732346463.23
    186 rdf:type schema:Person
    187 sg:pub.10.1007/978-1-4939-2760-9_29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041260983
    188 https://doi.org/10.1007/978-1-4939-2760-9_29
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1007/s00216-009-2664-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045598050
    191 https://doi.org/10.1007/s00216-009-2664-5
    192 rdf:type schema:CreativeWork
    193 sg:pub.10.1007/s00216-012-6690-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020197428
    194 https://doi.org/10.1007/s00216-012-6690-3
    195 rdf:type schema:CreativeWork
    196 sg:pub.10.1007/s10337-014-2813-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019339575
    197 https://doi.org/10.1007/s10337-014-2813-7
    198 rdf:type schema:CreativeWork
    199 sg:pub.10.1007/s10719-012-9376-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019594761
    200 https://doi.org/10.1007/s10719-012-9376-3
    201 rdf:type schema:CreativeWork
    202 sg:pub.10.1007/s13361-013-0610-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004464648
    203 https://doi.org/10.1007/s13361-013-0610-4
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1016/j.jasms.2005.01.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012784675
    206 https://doi.org/10.1016/j.jasms.2005.01.005
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1016/j.jasms.2005.01.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027892668
    209 https://doi.org/10.1016/j.jasms.2005.01.006
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1016/j.jasms.2008.11.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029202640
    212 https://doi.org/10.1016/j.jasms.2008.11.012
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1016/s1044-0305(00)00122-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016033386
    215 https://doi.org/10.1016/s1044-0305(00)00122-7
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1038/ncomms1333 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018591287
    218 https://doi.org/10.1038/ncomms1333
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1038/nprot.2012.063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035692290
    221 https://doi.org/10.1038/nprot.2012.063
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1186/1471-2105-15-s1-s9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018559057
    224 https://doi.org/10.1186/1471-2105-15-s1-s9
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1385/0-89603-355-4:53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083291092
    227 https://doi.org/10.1385/0-89603-355-4:53
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1385/1-59745-167-3:125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000245844
    230 https://doi.org/10.1385/1-59745-167-3:125
    231 rdf:type schema:CreativeWork
    232 grid-institutes:grid.1022.1 schema:alternateName Institute for Glycomics, Griffith University, QLD, 4222, Gold Coast, Australia
    233 schema:name Department of Chemistry and Biomolecular Sciences, Faculty of Science & Engineering, Macquarie University, North Ryde, 2109, Sydney, NSW, Australia
    234 Institute for Glycomics, Griffith University, QLD, 4222, Gold Coast, Australia
    235 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...