SPR and ITC determination of the kinetics and the thermodynamics of bivalent versus monovalent sugar ligand–lectin interactions View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-05

AUTHORS

Bandaru Narasimha Murthy, Sharmistha Sinha, Avadhesha Surolia, Shantinath S. Indi, Narayanaswamy Jayaraman

ABSTRACT

A kinetic study of the interaction of bivalent and monovalent sugar ligands with a lectin was undertaken with the aid of surface plasmon resonance (SPR) method. The study involved a series of bivalent alpha-D-mannopyranoside containing sugar ligands, with systematic variation in the distance between the sugar ligands. The detailed kinetic studies showed that bivalent ligands underwent a faster association (k(on)) and a slower dissociation (k(off)) of the ligand-lectin complexes, in comparison to the monovalent ligand-lectin complexes. The kinetic constants were complemented further by assessing the thermodynamic parameters with the aid of isothermal titration calorimetry (ITC). The initiation of cross-linking of ligand-lectin interactions emerge from the early stages of the complexation. The dynamic light scattering (DLS) and the transmission electron microscopy (TEM) techniques allowed judging the sizes and morphologies of the complex in the solution and solid states, respectively. More... »

PAGES

313-321

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10719-007-9076-6

DOI

http://dx.doi.org/10.1007/s10719-007-9076-6

DIMENSIONS

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PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17955363


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