Fine mapping of Ren3 reveals two loci mediating hypersensitive response against Erysiphe necator in grapevine View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-02-17

AUTHORS

Daniel Zendler, Pierre Schneider, Reinhard Töpfer, Eva Zyprian

ABSTRACT

Grapevine (Vitis vinifera L.) is economically very important for the production of wine, table grapes and raisins. However, grapevine is threatened by a brought range of pathogens. A destructive disease worldwide is powdery mildew caused by the ascomycete Erysiphe necator. In the grapevine cultivar `Regent’ a resistance locus against E. necator, Ren3, was previously reported. It spans an interval of approximately seven Mb on chromosome 15. We attempted to delimit this interval to facilitate its further molecular analysis. New simple sequence repeat markers targeted to the Ren3 region were designed. They were applied for fine mapping in the cross populations of ‘Regent’ × ‘Lemberger’ and ‘Regent’ × ‘Cabernet Sauvignon’ that segregate for E. necator resistance. Complementarily we scored E. necator infection levels of ‘Regent’ × ‘Lemberger’ progeny at different time points over the course of the vegetation period in 2015 and 2016. Subsequent QTL analysis revealed a maximum LOD value that shifted during the season from marker GF15-10 located at 2.2 Mb to marker GF15-53 located at 3.5 Mb and to marker ScORA7* located at 9.4 Mb on chromosome 15 (positions according to the grapevine reference genome of PN40024). To investigate the Ren3-encoded resistance mechanism we performed detached leaf infection assays for microscopic studies. These revealed that Ren3 carrying individuals react with a hypersensitive response. Results of detached leaf assays on recombinants in the Ren3 locus indicate that not only one, but two distinct genetic regions on chromosome 15 mediate hypersensitive response against E. necator. More... »

PAGES

68

References to SciGraph publications

  • 2010-12-28. Using a limited mapping strategy to identify major QTLs for resistance to grapevine powdery mildew (Erysiphe necator) and their use in marker-assisted breeding in THEORETICAL AND APPLIED GENETICS
  • 2006-10-17. Development of SCAR markers linked to powdery mildew (Uncinula necator) resistance in grapevine (Vitis vinifera L. and Vitis sp.) in MOLECULAR BREEDING
  • 2004-06-04. Mapping 245 SSR markers on the Vitis vinifera genome: a tool for grape genetics in THEORETICAL AND APPLIED GENETICS
  • 2014-12-09. Adaptive genomic structural variation in the grape powdery mildew pathogen, Erysiphe necator in BMC GENOMICS
  • 2011-12-23. A standardised method for the quantitative analysis of resistance to grapevine powdery mildew in EUROPEAN JOURNAL OF PLANT PATHOLOGY
  • 2011-04-08. Programmed cell death in the plant immune system in CELL DEATH & DIFFERENTIATION
  • 2016-04-02. Quantitative trait loci affecting pathogen resistance and ripening of grapevines in MOLECULAR GENETICS AND GENOMICS
  • 2009-12-30. The powdery mildew resistance gene REN1 co-segregates with an NBS-LRR gene cluster in two Central Asian grapevines in BMC GENETICS
  • 2010-09-01. Phylogeography and population structure of the grape powdery mildew fungus, Erysiphe necator, from diverse Vitis species in BMC EVOLUTIONARY BIOLOGY
  • 2007-12-07. Resistance to Erysiphe necator in the grapevine ‘Kishmish vatkana’ is controlled by a single locus through restriction of hyphal growth in THEORETICAL AND APPLIED GENETICS
  • 2005-12. Genetic Variation and Population Structure of the Grape Powdery Mildew Fungus, Erysiphe necator, in Southern France in EUROPEAN JOURNAL OF PLANT PATHOLOGY
  • 2005-05. Development and characterization of a large set of microsatellite markers in grapevine (Vitis vinifera L.) suitable for multiplex PCR in MOLECULAR BREEDING
  • 2009-10-11. Resistance to Plasmopara viticola in grapevine ‘Bianca’ is controlled by a major dominant gene causing localised necrosis at the infection site in THEORETICAL AND APPLIED GENETICS
  • 2011-07-07. Defence responses in Rpv3-dependent resistance to grapevine downy mildew in PLANTA
  • 2005-01. Isolation of (AC)n-microsatellites in Vitis vinifera L. and analysis of genetic background in grapevines under marker assisted selection in MOLECULAR BREEDING
  • 2014-08-12. QTL analysis of flowering time and ripening traits suggests an impact of a genomic region on linkage group 1 in Vitis in THEORETICAL AND APPLIED GENETICS
  • 2007-08-26. The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla in NATURE
  • 2006-11-16. Plant NBS-LRR proteins in pathogen sensing and host defense in NATURE IMMUNOLOGY
  • 2003-10-22. Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine in THEORETICAL AND APPLIED GENETICS
  • 2014-06-11. Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population in EUPHYTICA
  • 2015-05-20. Current understanding of grapevine defense mechanisms against the biotrophic fungus (Erysiphe necator), the causal agent of powdery mildew disease in HORTICULTURE RESEARCH
  • 2016-07-29. Identification of two novel powdery mildew resistance loci, Ren6 and Ren7, from the wild Chinese grape species Vitis piasezkii in BMC PLANT BIOLOGY
  • 2010-08-17. Genetic linkage maps of two interspecific grape crosses (Vitis spp.) used to localize quantitative trait loci for downy mildew resistance in TREE GENETICS & GENOMES
  • 2007-05-05. Genetic mapping and localization of quantitative trait loci affecting fungal disease resistance and leaf morphology in grapevine (Vitis vinifera L) in MOLECULAR BREEDING
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10681-017-1857-9

    DOI

    http://dx.doi.org/10.1007/s10681-017-1857-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1083894321


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Julius-K\u00fchn Institut, Institut f\u00fcr Rebenz\u00fcchtung Geilweilerhof, 76833, Siebeldingen, Germany", 
              "id": "http://www.grid.ac/institutes/grid.13946.39", 
              "name": [
                "Julius-K\u00fchn Institut, Institut f\u00fcr Rebenz\u00fcchtung Geilweilerhof, 76833, Siebeldingen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zendler", 
            "givenName": "Daniel", 
            "id": "sg:person.014445011017.84", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014445011017.84"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Julius-K\u00fchn Institut, Institut f\u00fcr Rebenz\u00fcchtung Geilweilerhof, 76833, Siebeldingen, Germany", 
              "id": "http://www.grid.ac/institutes/grid.13946.39", 
              "name": [
                "Julius-K\u00fchn Institut, Institut f\u00fcr Rebenz\u00fcchtung Geilweilerhof, 76833, Siebeldingen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schneider", 
            "givenName": "Pierre", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Julius-K\u00fchn Institut, Institut f\u00fcr Rebenz\u00fcchtung Geilweilerhof, 76833, Siebeldingen, Germany", 
              "id": "http://www.grid.ac/institutes/grid.13946.39", 
              "name": [
                "Julius-K\u00fchn Institut, Institut f\u00fcr Rebenz\u00fcchtung Geilweilerhof, 76833, Siebeldingen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "T\u00f6pfer", 
            "givenName": "Reinhard", 
            "id": "sg:person.0744241567.80", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744241567.80"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Julius-K\u00fchn Institut, Institut f\u00fcr Rebenz\u00fcchtung Geilweilerhof, 76833, Siebeldingen, Germany", 
              "id": "http://www.grid.ac/institutes/grid.13946.39", 
              "name": [
                "Julius-K\u00fchn Institut, Institut f\u00fcr Rebenz\u00fcchtung Geilweilerhof, 76833, Siebeldingen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zyprian", 
            "givenName": "Eva", 
            "id": "sg:person.0676126367.38", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676126367.38"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s00122-007-0680-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017068538", 
              "https://doi.org/10.1007/s00122-007-0680-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00438-016-1200-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005930552", 
              "https://doi.org/10.1007/s00438-016-1200-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11032-004-7651-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019889385", 
              "https://doi.org/10.1007/s11032-004-7651-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12870-016-0855-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005756027", 
              "https://doi.org/10.1186/s12870-016-0855-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-009-1167-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020261521", 
              "https://doi.org/10.1007/s00122-009-1167-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/hortres.2015.20", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029921680", 
              "https://doi.org/10.1038/hortres.2015.20"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11295-010-0322-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016816211", 
              "https://doi.org/10.1007/s11295-010-0322-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00425-011-1461-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049700745", 
              "https://doi.org/10.1007/s00425-011-1461-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11032-007-9097-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009344109", 
              "https://doi.org/10.1007/s11032-007-9097-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11032-006-9047-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021042206", 
              "https://doi.org/10.1007/s11032-006-9047-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cdd.2011.37", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030921204", 
              "https://doi.org/10.1038/cdd.2011.37"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10681-014-1167-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046405663", 
              "https://doi.org/10.1007/s10681-014-1167-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2156-10-89", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048446228", 
              "https://doi.org/10.1186/1471-2156-10-89"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-010-1511-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017001898", 
              "https://doi.org/10.1007/s00122-010-1511-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10658-011-9922-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013899791", 
              "https://doi.org/10.1007/s10658-011-9922-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature06148", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040664071", 
              "https://doi.org/10.1038/nature06148"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-014-2310-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000561244", 
              "https://doi.org/10.1007/s00122-014-2310-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-004-1704-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028610095", 
              "https://doi.org/10.1007/s00122-004-1704-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ni1410", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034869150", 
              "https://doi.org/10.1038/ni1410"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2164-15-1081", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005040312", 
              "https://doi.org/10.1186/1471-2164-15-1081"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10658-005-4563-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046247200", 
              "https://doi.org/10.1007/s10658-005-4563-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11032-004-1362-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016123931", 
              "https://doi.org/10.1007/s11032-004-1362-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2148-10-268", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039017305", 
              "https://doi.org/10.1186/1471-2148-10-268"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-003-1445-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049116493", 
              "https://doi.org/10.1007/s00122-003-1445-3"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017-02-17", 
        "datePublishedReg": "2017-02-17", 
        "description": "Grapevine (Vitis vinifera L.) is economically very important for the production of wine, table grapes and raisins. However, grapevine is threatened by a brought range of pathogens. A destructive disease worldwide is powdery mildew caused by the ascomycete Erysiphe necator. In the grapevine cultivar `Regent\u2019 a resistance locus against E. necator, Ren3, was previously reported. It spans an interval of approximately seven Mb on chromosome 15. We attempted to delimit this interval to facilitate its further molecular analysis. New simple sequence repeat markers targeted to the Ren3 region were designed. They were applied for fine mapping in the cross populations of \u2018Regent\u2019 \u00d7 \u2018Lemberger\u2019 and \u2018Regent\u2019 \u00d7 \u2018Cabernet Sauvignon\u2019 that segregate for E. necator resistance. Complementarily we scored E. necator infection levels of \u2018Regent\u2019 \u00d7 \u2018Lemberger\u2019 progeny at different time points over the course of the vegetation period in 2015 and 2016. Subsequent QTL analysis revealed a maximum LOD value that shifted during the season from marker GF15-10 located at 2.2\u00a0Mb to marker GF15-53 located at 3.5\u00a0Mb and to marker ScORA7* located at 9.4\u00a0Mb on chromosome 15 (positions according to the grapevine reference genome of PN40024). To investigate the Ren3-encoded resistance mechanism we performed detached leaf infection assays for microscopic studies. These revealed that Ren3 carrying individuals react with a hypersensitive response. Results of detached leaf assays on recombinants in the Ren3 locus indicate that not only one, but two distinct genetic regions on chromosome 15 mediate hypersensitive response against E. necator.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s10681-017-1857-9", 
        "inLanguage": "en", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1028679", 
            "issn": [
              "0014-2336", 
              "1573-5060"
            ], 
            "name": "Euphytica", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "213"
          }
        ], 
        "keywords": [
          "Erysiphe necator", 
          "new simple sequence repeat (SSR) markers", 
          "simple sequence repeat (SSR) markers", 
          "hypersensitive response", 
          "fine mapping", 
          "leaf infection assays", 
          "detached leaf assays", 
          "distinct genetic regions", 
          "production of wine", 
          "sequence repeat (SSR) markers", 
          "range of pathogens", 
          "subsequent QTL analysis", 
          "powdery mildew", 
          "table grapes", 
          "REN3", 
          "vegetation period", 
          "resistance loci", 
          "cross population", 
          "leaf assays", 
          "infection levels", 
          "destructive disease", 
          "grapevine cultivars", 
          "Cabernet Sauvignon", 
          "further molecular analysis", 
          "QTL analysis", 
          "grapevine", 
          "repeat (SSR) markers", 
          "GF15", 
          "Lemberger", 
          "infection assays", 
          "necator", 
          "resistance mechanisms", 
          "genetic regions", 
          "LOD values", 
          "mildew", 
          "cultivars", 
          "grapes", 
          "season", 
          "Sauvignon", 
          "molecular analysis", 
          "pathogens", 
          "progeny", 
          "production", 
          "raisins", 
          "recombinants", 
          "wine", 
          "resistance", 
          "response", 
          "population", 
          "region", 
          "mapping", 
          "regents", 
          "loci", 
          "MB", 
          "period", 
          "levels", 
          "disease", 
          "range", 
          "intervals", 
          "markers", 
          "values", 
          "chromosome 15", 
          "study", 
          "analysis", 
          "assays", 
          "results", 
          "individuals", 
          "different time points", 
          "mechanism", 
          "point", 
          "time points", 
          "microscopic study", 
          "course", 
          "ascomycete Erysiphe necator", 
          "Ren3 region", 
          "necator resistance", 
          "necator infection levels", 
          "maximum LOD value", 
          "ScORA7", 
          "detached leaf infection assays", 
          "chromosome 15 mediate hypersensitive response", 
          "mediate hypersensitive response"
        ], 
        "name": "Fine mapping of Ren3 reveals two loci mediating hypersensitive response against Erysiphe necator in grapevine", 
        "pagination": "68", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1083894321"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s10681-017-1857-9"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s10681-017-1857-9", 
          "https://app.dimensions.ai/details/publication/pub.1083894321"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2021-12-01T19:40", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_743.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s10681-017-1857-9"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10681-017-1857-9'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10681-017-1857-9'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10681-017-1857-9'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10681-017-1857-9'


     

    This table displays all metadata directly associated to this object as RDF triples.

    256 TRIPLES      22 PREDICATES      131 URIs      99 LITERALS      6 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s10681-017-1857-9 schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author Naf4c6923573347b2b3b95cbb67e44082
    4 schema:citation sg:pub.10.1007/s00122-003-1445-3
    5 sg:pub.10.1007/s00122-004-1704-y
    6 sg:pub.10.1007/s00122-007-0680-4
    7 sg:pub.10.1007/s00122-009-1167-2
    8 sg:pub.10.1007/s00122-010-1511-6
    9 sg:pub.10.1007/s00122-014-2310-2
    10 sg:pub.10.1007/s00425-011-1461-5
    11 sg:pub.10.1007/s00438-016-1200-5
    12 sg:pub.10.1007/s10658-005-4563-8
    13 sg:pub.10.1007/s10658-011-9922-z
    14 sg:pub.10.1007/s10681-014-1167-4
    15 sg:pub.10.1007/s11032-004-1362-4
    16 sg:pub.10.1007/s11032-004-7651-0
    17 sg:pub.10.1007/s11032-006-9047-9
    18 sg:pub.10.1007/s11032-007-9097-7
    19 sg:pub.10.1007/s11295-010-0322-x
    20 sg:pub.10.1038/cdd.2011.37
    21 sg:pub.10.1038/hortres.2015.20
    22 sg:pub.10.1038/nature06148
    23 sg:pub.10.1038/ni1410
    24 sg:pub.10.1186/1471-2148-10-268
    25 sg:pub.10.1186/1471-2156-10-89
    26 sg:pub.10.1186/1471-2164-15-1081
    27 sg:pub.10.1186/s12870-016-0855-8
    28 schema:datePublished 2017-02-17
    29 schema:datePublishedReg 2017-02-17
    30 schema:description Grapevine (Vitis vinifera L.) is economically very important for the production of wine, table grapes and raisins. However, grapevine is threatened by a brought range of pathogens. A destructive disease worldwide is powdery mildew caused by the ascomycete Erysiphe necator. In the grapevine cultivar `Regent’ a resistance locus against E. necator, Ren3, was previously reported. It spans an interval of approximately seven Mb on chromosome 15. We attempted to delimit this interval to facilitate its further molecular analysis. New simple sequence repeat markers targeted to the Ren3 region were designed. They were applied for fine mapping in the cross populations of ‘Regent’ × ‘Lemberger’ and ‘Regent’ × ‘Cabernet Sauvignon’ that segregate for E. necator resistance. Complementarily we scored E. necator infection levels of ‘Regent’ × ‘Lemberger’ progeny at different time points over the course of the vegetation period in 2015 and 2016. Subsequent QTL analysis revealed a maximum LOD value that shifted during the season from marker GF15-10 located at 2.2 Mb to marker GF15-53 located at 3.5 Mb and to marker ScORA7* located at 9.4 Mb on chromosome 15 (positions according to the grapevine reference genome of PN40024). To investigate the Ren3-encoded resistance mechanism we performed detached leaf infection assays for microscopic studies. These revealed that Ren3 carrying individuals react with a hypersensitive response. Results of detached leaf assays on recombinants in the Ren3 locus indicate that not only one, but two distinct genetic regions on chromosome 15 mediate hypersensitive response against E. necator.
    31 schema:genre article
    32 schema:inLanguage en
    33 schema:isAccessibleForFree false
    34 schema:isPartOf N4045ecef3e704abab70a70fe6f9a3b04
    35 N9c3b6d4ba00d4dda9a290e82038d4006
    36 sg:journal.1028679
    37 schema:keywords Cabernet Sauvignon
    38 Erysiphe necator
    39 GF15
    40 LOD values
    41 Lemberger
    42 MB
    43 QTL analysis
    44 REN3
    45 Ren3 region
    46 Sauvignon
    47 ScORA7
    48 analysis
    49 ascomycete Erysiphe necator
    50 assays
    51 chromosome 15
    52 chromosome 15 mediate hypersensitive response
    53 course
    54 cross population
    55 cultivars
    56 destructive disease
    57 detached leaf assays
    58 detached leaf infection assays
    59 different time points
    60 disease
    61 distinct genetic regions
    62 fine mapping
    63 further molecular analysis
    64 genetic regions
    65 grapes
    66 grapevine
    67 grapevine cultivars
    68 hypersensitive response
    69 individuals
    70 infection assays
    71 infection levels
    72 intervals
    73 leaf assays
    74 leaf infection assays
    75 levels
    76 loci
    77 mapping
    78 markers
    79 maximum LOD value
    80 mechanism
    81 mediate hypersensitive response
    82 microscopic study
    83 mildew
    84 molecular analysis
    85 necator
    86 necator infection levels
    87 necator resistance
    88 new simple sequence repeat (SSR) markers
    89 pathogens
    90 period
    91 point
    92 population
    93 powdery mildew
    94 production
    95 production of wine
    96 progeny
    97 raisins
    98 range
    99 range of pathogens
    100 recombinants
    101 regents
    102 region
    103 repeat (SSR) markers
    104 resistance
    105 resistance loci
    106 resistance mechanisms
    107 response
    108 results
    109 season
    110 sequence repeat (SSR) markers
    111 simple sequence repeat (SSR) markers
    112 study
    113 subsequent QTL analysis
    114 table grapes
    115 time points
    116 values
    117 vegetation period
    118 wine
    119 schema:name Fine mapping of Ren3 reveals two loci mediating hypersensitive response against Erysiphe necator in grapevine
    120 schema:pagination 68
    121 schema:productId N4d0de3c30e604519bd2775fdbec1e8ff
    122 N89aec421584c4bd39fcff16e31cbd24d
    123 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083894321
    124 https://doi.org/10.1007/s10681-017-1857-9
    125 schema:sdDatePublished 2021-12-01T19:40
    126 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    127 schema:sdPublisher N78d28523d3bf41b9ab63a5cd77af2a35
    128 schema:url https://doi.org/10.1007/s10681-017-1857-9
    129 sgo:license sg:explorer/license/
    130 sgo:sdDataset articles
    131 rdf:type schema:ScholarlyArticle
    132 N4045ecef3e704abab70a70fe6f9a3b04 schema:volumeNumber 213
    133 rdf:type schema:PublicationVolume
    134 N4d0de3c30e604519bd2775fdbec1e8ff schema:name doi
    135 schema:value 10.1007/s10681-017-1857-9
    136 rdf:type schema:PropertyValue
    137 N51d0dee1176c4556a2be6ec5756d41fa rdf:first sg:person.0744241567.80
    138 rdf:rest Nb4bc0faec41c407abc44197afc9c1db4
    139 N78d28523d3bf41b9ab63a5cd77af2a35 schema:name Springer Nature - SN SciGraph project
    140 rdf:type schema:Organization
    141 N89aec421584c4bd39fcff16e31cbd24d schema:name dimensions_id
    142 schema:value pub.1083894321
    143 rdf:type schema:PropertyValue
    144 N9c3b6d4ba00d4dda9a290e82038d4006 schema:issueNumber 3
    145 rdf:type schema:PublicationIssue
    146 Naf4c6923573347b2b3b95cbb67e44082 rdf:first sg:person.014445011017.84
    147 rdf:rest Nda435b0d2feb4ca6bff4ba733e361f42
    148 Nb4bc0faec41c407abc44197afc9c1db4 rdf:first sg:person.0676126367.38
    149 rdf:rest rdf:nil
    150 Nc1a293b65ec04b9ca264b5432f2a1c32 schema:affiliation grid-institutes:grid.13946.39
    151 schema:familyName Schneider
    152 schema:givenName Pierre
    153 rdf:type schema:Person
    154 Nda435b0d2feb4ca6bff4ba733e361f42 rdf:first Nc1a293b65ec04b9ca264b5432f2a1c32
    155 rdf:rest N51d0dee1176c4556a2be6ec5756d41fa
    156 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    157 schema:name Biological Sciences
    158 rdf:type schema:DefinedTerm
    159 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    160 schema:name Genetics
    161 rdf:type schema:DefinedTerm
    162 sg:journal.1028679 schema:issn 0014-2336
    163 1573-5060
    164 schema:name Euphytica
    165 schema:publisher Springer Nature
    166 rdf:type schema:Periodical
    167 sg:person.014445011017.84 schema:affiliation grid-institutes:grid.13946.39
    168 schema:familyName Zendler
    169 schema:givenName Daniel
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014445011017.84
    171 rdf:type schema:Person
    172 sg:person.0676126367.38 schema:affiliation grid-institutes:grid.13946.39
    173 schema:familyName Zyprian
    174 schema:givenName Eva
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676126367.38
    176 rdf:type schema:Person
    177 sg:person.0744241567.80 schema:affiliation grid-institutes:grid.13946.39
    178 schema:familyName Töpfer
    179 schema:givenName Reinhard
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744241567.80
    181 rdf:type schema:Person
    182 sg:pub.10.1007/s00122-003-1445-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049116493
    183 https://doi.org/10.1007/s00122-003-1445-3
    184 rdf:type schema:CreativeWork
    185 sg:pub.10.1007/s00122-004-1704-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1028610095
    186 https://doi.org/10.1007/s00122-004-1704-y
    187 rdf:type schema:CreativeWork
    188 sg:pub.10.1007/s00122-007-0680-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017068538
    189 https://doi.org/10.1007/s00122-007-0680-4
    190 rdf:type schema:CreativeWork
    191 sg:pub.10.1007/s00122-009-1167-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020261521
    192 https://doi.org/10.1007/s00122-009-1167-2
    193 rdf:type schema:CreativeWork
    194 sg:pub.10.1007/s00122-010-1511-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017001898
    195 https://doi.org/10.1007/s00122-010-1511-6
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1007/s00122-014-2310-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000561244
    198 https://doi.org/10.1007/s00122-014-2310-2
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1007/s00425-011-1461-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049700745
    201 https://doi.org/10.1007/s00425-011-1461-5
    202 rdf:type schema:CreativeWork
    203 sg:pub.10.1007/s00438-016-1200-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005930552
    204 https://doi.org/10.1007/s00438-016-1200-5
    205 rdf:type schema:CreativeWork
    206 sg:pub.10.1007/s10658-005-4563-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046247200
    207 https://doi.org/10.1007/s10658-005-4563-8
    208 rdf:type schema:CreativeWork
    209 sg:pub.10.1007/s10658-011-9922-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1013899791
    210 https://doi.org/10.1007/s10658-011-9922-z
    211 rdf:type schema:CreativeWork
    212 sg:pub.10.1007/s10681-014-1167-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046405663
    213 https://doi.org/10.1007/s10681-014-1167-4
    214 rdf:type schema:CreativeWork
    215 sg:pub.10.1007/s11032-004-1362-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016123931
    216 https://doi.org/10.1007/s11032-004-1362-4
    217 rdf:type schema:CreativeWork
    218 sg:pub.10.1007/s11032-004-7651-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019889385
    219 https://doi.org/10.1007/s11032-004-7651-0
    220 rdf:type schema:CreativeWork
    221 sg:pub.10.1007/s11032-006-9047-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021042206
    222 https://doi.org/10.1007/s11032-006-9047-9
    223 rdf:type schema:CreativeWork
    224 sg:pub.10.1007/s11032-007-9097-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009344109
    225 https://doi.org/10.1007/s11032-007-9097-7
    226 rdf:type schema:CreativeWork
    227 sg:pub.10.1007/s11295-010-0322-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016816211
    228 https://doi.org/10.1007/s11295-010-0322-x
    229 rdf:type schema:CreativeWork
    230 sg:pub.10.1038/cdd.2011.37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030921204
    231 https://doi.org/10.1038/cdd.2011.37
    232 rdf:type schema:CreativeWork
    233 sg:pub.10.1038/hortres.2015.20 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029921680
    234 https://doi.org/10.1038/hortres.2015.20
    235 rdf:type schema:CreativeWork
    236 sg:pub.10.1038/nature06148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040664071
    237 https://doi.org/10.1038/nature06148
    238 rdf:type schema:CreativeWork
    239 sg:pub.10.1038/ni1410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034869150
    240 https://doi.org/10.1038/ni1410
    241 rdf:type schema:CreativeWork
    242 sg:pub.10.1186/1471-2148-10-268 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039017305
    243 https://doi.org/10.1186/1471-2148-10-268
    244 rdf:type schema:CreativeWork
    245 sg:pub.10.1186/1471-2156-10-89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048446228
    246 https://doi.org/10.1186/1471-2156-10-89
    247 rdf:type schema:CreativeWork
    248 sg:pub.10.1186/1471-2164-15-1081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005040312
    249 https://doi.org/10.1186/1471-2164-15-1081
    250 rdf:type schema:CreativeWork
    251 sg:pub.10.1186/s12870-016-0855-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005756027
    252 https://doi.org/10.1186/s12870-016-0855-8
    253 rdf:type schema:CreativeWork
    254 grid-institutes:grid.13946.39 schema:alternateName Julius-Kühn Institut, Institut für Rebenzüchtung Geilweilerhof, 76833, Siebeldingen, Germany
    255 schema:name Julius-Kühn Institut, Institut für Rebenzüchtung Geilweilerhof, 76833, Siebeldingen, Germany
    256 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...