Cloning and characterization of repetitive sequences and development of SCAR markers specific for the P genome of Agropyron cristatum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-04

AUTHORS

M. Wu, J. P. Zhang, J. C. Wang, X. M. Yang, A. N. Gao, X. K. Zhang, W. H. Liu, L. H. Li

ABSTRACT

In previous studies, we successfully transferred the P genome of Agropyron cristatum into wheat using wide hybridization methods. In the current investigation, repetitive sequences were cloned and DNA markers specific for the P genome of A. cristatum were developed. Three P genome-specific markers, designated OPX07-1036, OPX11-817 and OPC05-1539, were identified and isolated using random amplified polymorphic DNAs. The three markers were successfully amplified in all tested materials that contained Agropyron chromatin, such as Agropyron itself and wheat-Agropyron addition lines. These RAPD markers were converted into SCAR markers to be used in detection of P genome chromatin in wheat. In situ probing with fluorescent-labeled marker DNA has shown that they are distributed in all arms of Agropyron hence they will be useful in a variety of studies on introgressions of the P-genome chromatin into wheat. More... »

PAGES

363-372

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10681-009-0033-2

DOI

http://dx.doi.org/10.1007/s10681-009-0033-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037996920


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