Agrobacterium-mediated transformation of faba bean (Vicia faba L.) using embryo axes View Full Text


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Article Info

DATE

2005-04

AUTHORS

Moemen Hanafy, Thomas Pickardt, Heiko Kiesecker, Hans-Joerg Jacobsen

ABSTRACT

A system for the production of transgenic faba bean by Agrobacterium-mediated transformation was developed. This system is based upon direct shoot organogenesis after transformation of meristematic cells derived from embryo axes. Explants were co-cultivated with A. tumefaciens strain EHA105/pGlsfa, which harbored a binary vector containing a gene encoding a sulphur rich sunflower albumin (SFA8) linked to the bar gene. Strain EHA 101/pAN109 carrying the binary plasmid containing the coding sequence of a mutant aspartate kinase gene (lysC) from E. coli in combination with neomycinphosphotransferase II gene (nptII) was used as well. The coding sequences of SFA8 and LysC genes were fused to seed specific promoters, either Vicia faba legumin B4 promoter (LeB4) or phaseolin promoter, respectively. Seven phosphinothricin (PPT) resistant clones from Mythos and Albatross cultivars were recovered. Integration, inheritance and expression of the transgenes were confirmed by Southern blot, PCR, enzyme activity assay and Western blot. More... »

PAGES

227-236

References to SciGraph publications

  • 1989-08. In vitro propagation of Vicia faba L. by micro-cutting and multiple shoot induction in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-02. Transformation of Vicia narbonensis via Agrobacterium-mediated gene transfer in PLANT CELL REPORTS
  • 1989-02. Plant regeneration via somatic embryogenesis inVicia narbonensis in PROTOPLASMA
  • 2003-03. Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method in PLANTA
  • 1990-08. Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens — mediated gene transfer in THEORETICAL AND APPLIED GENETICS
  • 2001-10. Agrobacterium-mediated transformation of Vicia faba in MOLECULAR BREEDING
  • 1995-09. Seed specific expression of the 2S albumin gene from Brazil nut (Bertholletia excelsa) in transgenicVicia narbonensis in MOLECULAR BREEDING
  • 1995-12. Thidiazuron-induced plant regeneration from protoplasts of Vicia faba cv. Mythos in PLANT CELL REPORTS
  • 1997-02. Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray in THEORETICAL AND APPLIED GENETICS
  • 1995-02. The sulphur-rich Brazil nut 2S albumin is specifically formed in transgenic seeds of the grain legume Vicia narbonensis in EUPHYTICA
  • 1988-08. Stable Transformation of Soybean (Glycine Max) by Particle Acceleration in NATURE BIOTECHNOLOGY
  • 1997-05. A simple system for pea transformation in PLANT CELL REPORTS
  • 1993-05. Stable transformation via particle bombardment in two different soybean regeneration systems in PLANT CELL REPORTS
  • 1996. Genetic Stability and Expression of Foreign Genes in Transgenic Lines of the Legume Vicia narbonensis in TRANSGENIC ORGANISMS AND BIOSAFETY
  • 2000-01. Agrobacterium mediated transformation of chickpea (Cicer arietinum L.) embryo axes in PLANT CELL REPORTS
  • 1992-07. Inheritance of a bacterial hygromycin phosphotransferase gene in the progeny of primary transgenic pea plants in THEORETICAL AND APPLIED GENETICS
  • 1997-10. Transformation of a grain legume (Lupinus angustifolius L.) via Agrobacterium tumefaciens-mediated gene transfer to shoot apices in MOLECULAR BREEDING
  • 1996-05. Production of fertile transgenic peanut (Arachis hypogaea L.) plants using Agrobacterium tumefaciens in PLANT CELL REPORTS
  • 1992-12. New plant binary vectors with selectable markers located proximal to the left T-DNA border in PLANT MOLECULAR BIOLOGY
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 2000-11. Agrobacterium tumefaciens– mediated transformation of soybean [Glycine max (L.) Merrill.] using immature zygotic cotyledon explants in PLANT CELL REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10681-005-1690-4

    DOI

    http://dx.doi.org/10.1007/s10681-005-1690-4

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    https://app.dimensions.ai/details/publication/pub.1047167529


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