Using the Flow Cytometry to Quantify the Giardia Cysts and Cryptosporidium Oocysts in Water Samples View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-05

AUTHORS

Bing-Mu Hsu, Nan-Min Wu, Hung-Der Jang, Feng-Cheng Shih, Min-Tao Wan, Chien-Min Kung

ABSTRACT

The flow cytometry (FC) has been used to detect Giardia cysts and Cryptosporidium oocysts quantitatively and instantaneously in this study. The experimental results showed that FC is potential to become a more precise method for the detection of Giardia and Cryptosporidium in water. This study also evaluated the staining efficiencies for three commercial antibodies. After staining Cryptosporidium oocysts with direct immunofluorescent antibodies in water samples, two populations were detected in the scatter-plots (FL1 versus SSC) of the FC. The Cryptosporidium oocysts and Giardia cysts are significantly separated from other particles while stained with direct immunofluorescent antibodies produced by Meridian Diagnostics and Waterborne Inc. More... »

PAGES

155-162

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10661-005-1608-6

DOI

http://dx.doi.org/10.1007/s10661-005-1608-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001253901

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15931984


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