Bimetallic blends and chitosan nanocomposites: novel antifungal agents against cotton seedling damping-off View Full Text


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Article Info

DATE

2017-10-02

AUTHORS

Kamel A. Abd-Elsalam, Alexander Yu. Vasil’kov, Ernest E. Said-Galiev, Margarita S. Rubina, Alexei R. Khokhlov, Alexander V. Naumkin, Eleonora V. Shtykova, Mousa A. Alghuthaymi

ABSTRACT

Phytopathological studies of chitosan nanocomposites are mainly focused on in vitro efficiency, so it is essential to perform a complementary greenhouse assay to find eco-friendly alternatives for plant disease management. In the present study, Cu-chitosan and Zn-chitosan nanocomposites were prepared by reduction of metal precursors in the presence of chitosan in sc CO2 medium and deposition of organosol on chitosan, respectively. Physicochemical properties of the nanocomposites were characterized by X-ray fluorescence analysis (XRF), Small angles X-ray Scattering (SAXS), X-ray Photoelectron spectroscopy (XPS), and Transmission electron microscopy (TEM). The bimetallic blends (BBs) based on nanoscale Cu(OH)2 were obtained through simple precipitation and grinding methods. In vitro and in vivo studies of the antifungal activity of Cu-chitosan, Zn-chitosan and BBs at concentrations of 30, 60, and 100 μg ml−1 were conducted against two anastomosis groups of Rhizoctonia solani for control of cotton seedling damping-off. Effect of metal-chitosan nanocomposites at 100 μg ml−1 combined with Cu-tolerant Trichoderma longibrachiatum strains was also evaluated for control of cotton seedling damping-off under greenhouse conditions. The BBs and Cu-chitosan nanocomposite showed the highest antifungal efficacy against both anastomosis groups of R. solani in vitro. These results indicated that BBs, Cu-chitosan nanocomposite, and BBs combined with Trichoderma may suppress cotton seedling disease caused by R. solani in vivo. The evaluation of R. solani in a greenhouse with a Trichoderma strain showed synergistic inhibitory effect with BBs. Light micrographs of mycelia treated with BBs showed the disruption of the hyphal structures. The interaction of the nanocomposites with DNA isolated from the exposed fungal cells, by means of bonding and/or degradation, was also investigated. DNA interaction in terms of binding and degradation for treated DNA with BBs and chitosan nanocomposites was demonstrated. The results showed the absence of DNA amplification by a microsatellite primed PCR. More... »

PAGES

57-72

References to SciGraph publications

  • 2011-05-25. Composite materials for medical purposes based on polyvinylpyrrolidone modified with ketoprofen and silver nanoparticles in RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY A
  • 2014-08-08. Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo in JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE A
  • 2015-01-10. Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds in APPLIED NANOSCIENCE
  • 2013-12-05. Bioactivity, mechanism of action, and cytotoxicity of copper-based nanoparticles: A review in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2003-11-01. Chitosan improves development, and protects Vitis vinifera L. against Botrytis cinerea in PLANT CELL REPORTS
  • 2007-06. Isolation of high-quality DNA from cotton and its fungal pathogens in JOURNAL OF PLANT DISEASES AND PROTECTION
  • 2011-06-18. Synthesis of Ag and Cu-chitosan metal-polymer nanocomposites in supercritical carbon dioxide medium and study of their structure and antimicrobial activity in NANOBIOTECHNOLOGY REPORTS
  • 2014-07-14. A residue-free green synergistic antifungal nanotechnology for pesticide thiram by ZnO nanoparticles in SCIENTIFIC REPORTS
  • 2012-08-30. Antifungal effect and mechanism of chitosan against the rice sheath blight pathogen, Rhizoctonia solani in BIOTECHNOLOGY LETTERS
  • 2012-08-26. Structure of mono- and bimetallic heterogeneous catalysts based on noble metals obtained by means of fluid technology and metal-vapor synthesis in RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY A
  • 2015-02-05. Chitosan as a promising natural compound to enhance potential physiological responses in plant: a review in PLANT PHYSIOLOGY REPORTS
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    33 Rhizoctonia solani
    34 SC-CO2 medium
    35 Trichoderma
    36 Trichoderma strains
    37 X-ray fluorescence analysis
    38 X-ray photoelectron spectroscopy
    39 X-ray scattering
    40 absence
    41 activity
    42 agents
    43 alternative
    44 amplification
    45 analysis
    46 anastomosis group
    47 angle X-ray scattering
    48 antifungal activity
    49 antifungal agents
    50 antifungal efficacy
    51 binding
    52 blends
    53 bonding
    54 cells
    55 chitosan
    56 chitosan nanocomposite
    57 concentration
    58 conditions
    59 control
    60 control of cotton
    61 cotton
    62 damping
    63 degradation
    64 deposition
    65 disease
    66 disease management
    67 disruption
    68 eco-friendly alternative
    69 effect
    70 efficacy
    71 efficiency
    72 electron microscopy
    73 evaluation
    74 fluorescence analysis
    75 fungal cells
    76 greenhouse
    77 greenhouse conditions
    78 group
    79 high antifungal efficacy
    80 hyphal structures
    81 inhibitory effect
    82 interaction
    83 light micrographs
    84 management
    85 means
    86 medium
    87 metal precursors
    88 method
    89 micrographs
    90 microsatellites
    91 microscopy
    92 mycelium
    93 nanocomposites
    94 nanoscale
    95 novel antifungal agents
    96 organosols
    97 photoelectron spectroscopy
    98 physicochemical properties
    99 phytopathological studies
    100 plant disease management
    101 precipitation
    102 precursors
    103 presence
    104 presence of chitosan
    105 present study
    106 properties
    107 reduction
    108 results
    109 scattering
    110 simple precipitation
    111 small-angle X-ray scattering
    112 solani
    113 spectroscopy
    114 strains
    115 structure
    116 study
    117 synergistic inhibitory effect
    118 terms
    119 terms of binding
    120 transmission electron microscopy
    121 vitro
    122 vivo
    123 vivo studies
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