In vitro cultivation of primary intestinal cells from Eisenia fetida as basis for ecotoxicological studies View Full Text


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Article Info

DATE

2021-11-17

AUTHORS

Simon A. B. Riedl, Matthias Völkl, Anja Holzinger, Julia Jasinski, Valérie Jérôme, Thomas Scheibel, Heike Feldhaar, Ruth Freitag

ABSTRACT

The earthworm Eisenia fetida is a commonly used model organism for unspecific soil feeders in ecotoxicological studies. Its intestinal cells are the first to encounter possible pollutants co-ingested by the earthworm, which makes them prime candidates for studies of toxic effects of environmental pollutants on the cellular as compared to the organismic level. In this context, the aim of this study was to demonstrate the suitability of preparations of primary intestinal E. fetida cells for in vitro ecotoxicological studies. For this purpose, a suitable isolation and cultivation protocol was established. Cells were isolated directly from the intestine, maintaining >85% viability during subsequent cultivations (up to 144 h). Exposure to established pollutants and soil elutriates comprising silver nanoparticles and metal ions (Cu2+, Cd2+) induced a significant decrease in the metabolic activity of the cells. In case of microplastic particles (MP particles), namely 0.2, 0.5, 2.0, and 3.0 µm diameter polystyrene (PS) beads as well as 0.5 and 2.0 µm diameter polylactic acid (PLA) beads, no active uptake was observed. Slight positive as well as negative dose and size dependent effects on the metabolism were seen, which to some extent might correlate with effects on the organismic level. More... »

PAGES

221-233

References to SciGraph publications

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  • 2016-08-31. Evaluation of cell binding to collagen and gelatin: a study of the effect of 2D and 3D architecture and surface chemistry in JOURNAL OF MATERIALS SCIENCE: MATERIALS IN MEDICINE
  • 2015-03-12. Establishment of toxicity thresholds in subpopulations of coelomocytes (amoebocytes vs. eleocytes) of Eisenia fetida exposed in vitro to a variety of metals: implications for biomarker measurements in ECOTOXICOLOGY
  • 2013-08-28. Establishment of an in vitro culture system for intestinal epithelial cells from Pheretima aspergillum (E. Perrier) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 1975. Response of Earthworm Leukocytes to Concanavalin a and Transplantation Antigens in IMMUNOLOGIC PHYLOGENY
  • 2018-10-01. Oxidative stress, energy metabolism and molecular responses of earthworms (Eisenia fetida) exposed to low-density polyethylene microplastics in ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • 2021-08-03. Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells in SCIENTIFIC REPORTS
  • 2014-07-11. Optimization of NRU assay in primary cultures of Eisenia fetida for metal toxicity assessment in ECOTOXICOLOGY
  • 1963-08. Heat-death Temperature in Allolobophora terrestris (Sav.) forma longa (Ude) and Eisenia foetida (Sav.) in NATURE
  • 2015-07-28. Effects of metals on earthworm life cycles: a review in ENVIRONMENTAL MONITORING AND ASSESSMENT
  • 2018-12-18. Identification and quantification of macro- and microplastics on an agricultural farmland in SCIENTIFIC REPORTS
  • 2021-02-12. Is cell culture a suitable tool for the evaluation of micro- and nanoplastics ecotoxicity? in ECOTOXICOLOGY
  • 2016-09-10. Uptake route and resulting toxicity of silver nanoparticles in Eisenia fetida earthworm exposed through Standard OECD Tests in ECOTOXICOLOGY
  • 1985-11. Toxicity of metals to the earthworm Eisenia fetida in BIOLOGY AND FERTILITY OF SOILS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10646-021-02495-2

    DOI

    http://dx.doi.org/10.1007/s10646-021-02495-2

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/34791607


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    72 model organisms
    73 nanoparticles
    74 organismic level
    75 organisms
    76 particles
    77 pollutants
    78 polylactic acid
    79 polystyrene
    80 possible pollutants
    81 preparation
    82 primary intestinal cells
    83 prime candidate
    84 protocol
    85 purpose
    86 significant decrease
    87 silver nanoparticles
    88 size-dependent effects
    89 soil elutriates
    90 soil feeders
    91 study
    92 subsequent cultivation
    93 suitability
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    95 toxic effects
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    97 viability
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