Study on metal binding capacity of the freshwater crab Sinopotamon henanense’s recombinant copper specific binding metallothionein expressed in Escherichia coli View Full Text


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Article Info

DATE

2021-11-09

AUTHORS

Lu Wang, Hui Zhen Yang, Wen Li Ma, Chien Min Chen, Lan Wang

ABSTRACT

The copper specific binding metallothionein (CuMT) is a type of cysteine-rich, metal-binding, small protein which plays an important role in Cu2+ metabolism in vertebrates. In this study, we investigated the metal tolerance and removing ability of recombinant strains harboring CuMT obtained in vivo from the freshwater crab Sinopotamon henanense (ShCuMT) in order to study its physiological functions and metal binding capacity. We performed a 3D modeling of ShCuMT and created its structural and functional models using the I‐TASSER program. The shCumt gene was inserted into a pGEX-4t-1 vector and recombinant soluble ShCuMT was expressed in Escherichia coli. In addition, in order to characterize the tolerance and removing ability of heavy metals in E. coli with ShCuMT expression, the recombinant strains harboring ShCuMT were exposed to various concentrations of Cd2+, Cu2+ and Zn2+, respectively. The results showed that ShCuMT contains transition metal binding sites. In addition, E. coli cells expressing ShCuMT exhibited enhanced metal tolerance and higher removing ability of metal ions than control cells. However, compared with Cd2+ and Zn2+, E. coli cells expressing ShCuMT have stronger tolerance and higher removing ability of Cu2+. In general, ShCuMT contains multiple transition metal binding sites, and it could enhance tolerance and removing ability of metal ions. Therefore, ShCuMT can provide potential candidates for heavy metal bioremediation. This research on the metal binding properties of ShCuMT provides a scientific basis for bioremediation of heavy metal pollution by the recombinant strains. More... »

PAGES

149-160

References to SciGraph publications

  • 2011-05-31. Physiological relevance and contribution to metal balance of specific and non-specific Metallothionein isoforms in the garden snail, Cantareus aspersus in BIOMETALS
  • 2008-08-24. Identification of a copper-binding metallothionein in pathogenic mycobacteria in NATURE CHEMICAL BIOLOGY
  • 2012-10-22. Advances in the Application of Plant Growth-Promoting Rhizobacteria in Phytoremediation of Heavy Metals in REVIEWS OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY VOLUME 223
  • 2012-06-19. A novel metallothionein gene from a mangrove plant Kandelia candel in ECOTOXICOLOGY
  • 2014-04-01. Cognate and noncognate metal ion coordination in metal-specific metallothioneins: the Helix pomatia system as a model in JBIC JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
  • 2010-04-02. The metal-binding properties of the blue crab copper specific CuMT-2: a crustacean metallothionein with two cysteine triplets in JBIC JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
  • 2017-05-24. The use of bacterial bioremediation of metals in aquatic environments in the twenty-first century: a systematic review in ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • 2017-09-10. Contaminant characteristics and environmental risk assessment of heavy metals in the paddy soils from lead (Pb)-zinc (Zn) mining areas in Guangdong Province, South China in ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • 2013-11-27. Expression of metallothionein of freshwater crab (Sinopotamon henanense) in Escherichia coli enhances tolerance and accumulation of zinc, copper and cadmium in ECOTOXICOLOGY
  • 2013-11-18. Copper: Effects of Deficiency and Overload in INTERRELATIONS BETWEEN ESSENTIAL METAL IONS AND HUMAN DISEASES
  • 1983-11. Haemocyanin concentration of juvenile lobsters (Homarus gammarus) in relation to moulting cycle and feeding conditions in MARINE BIOLOGY
  • 2011-08-08. Zn- and Cu-thioneins: a functional classification for metallothioneins? in JBIC JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
  • 2020-02-10. Cadmium behavior in a karst environment hydrological cycle in ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • 2018-07-14. Biominerlisation as a Remediation Technique: A Critical Review in GEOTECHNICAL CHARACTERISATION AND GEOENVIRONMENTAL ENGINEERING
  • 2008-10. A bacterial copper metallothionein in NATURE CHEMICAL BIOLOGY
  • 2003-09-09. Time-course of cadmium-induced acute hepatotoxicity in the rat liver: the role of apoptosis in ARCHIVES OF TOXICOLOGY
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    http://scigraph.springernature.com/pub.10.1007/s10646-021-02470-x

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    http://dx.doi.org/10.1007/s10646-021-02470-x

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/34751855


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