Deguelin, an Akt Inhibitor, Down-Regulates NF-κB Signaling and Induces Apoptosis in Colon Cancer Cells and Inhibits Tumor Growth in Mice View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-11

AUTHORS

Hyoun Woo Kang, Jung Mogg Kim, Mi Yeon Cha, Hyun Chae Jung, In Sung Song, Joo Sung Kim

ABSTRACT

BACKGROUND: Deguelin, a naturally occurring rotenoid, is known to be an Akt inhibitor and to have an anti-tumor effect on several cancers. AIMS: This study was performed to elucidate the effect of deguelin on apoptotic pathways related to NF-κB signaling in colon cancer cells and on the anti-tumor effect in colon cancer xenograft mice. METHODS: We studied COLO 205 and HCT116 cells in the presence or absence of deguelin. NF-κB signaling was examined by real-time RT-PCR for interleukin (IL)-8, by Western blotting for IκB phosphorylation/degradation, and by the electrophoretic mobility shift assay. Cell death was determined by the MTT assay, and apoptosis by Annexin V-FITC staining and caspase-3 activity. We also assessed the expression of antiapoptotic and proapoptotic factors by use of RT-PCR. In colon cancer xenograft mice, we evaluated the effect of deguelin on inoculated tumor growth, and apoptotic index was measured by the in vivo TUNEL assay. RESULTS: Deguelin significantly inhibited IL-8 gene expression, IκB phosphorylation/degradation, and DNA binding activity of NF-κB in colon cancer cells. Deguelin induced cell death and apoptosis in colon cancer cells in a dose and time-dependent manner. Deguelin down-regulated expression of NF-κB-mediated antiapoptotic factors such as cFLIP, Bcl-2, and Bcl-X(L). In the colon cancer xenograft model, the volume of the tumor treated with deguelin was significantly lower than that of the control, and the apoptotic index for deguelin-treated mice was much higher. CONCLUSION: Deguelin might be a potential therapeutic agent for treatment of colorectal cancer. More... »

PAGES

2873-2882

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10620-012-2237-x

DOI

http://dx.doi.org/10.1007/s10620-012-2237-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044389101

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22623043


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blotting, Western", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CASP8 and FADD-Like Apoptosis Regulating Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caspase 3", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colonic Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Down-Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Situ Nick-End Labeling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Interleukin-8", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "NF-kappa B", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proto-Oncogene Proteins c-bcl-2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Real-Time Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rotenone", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Statistics, Nonparametric", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "bcl-X Protein", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Dongguk University Ilsan Hospital", 
          "id": "https://www.grid.ac/institutes/grid.470090.a", 
          "name": [
            "Department of Internal Medicine, Dongguk University Ilsan Hospital, Seoul, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kang", 
        "givenName": "Hyoun Woo", 
        "id": "sg:person.01312026504.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312026504.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hanyang University", 
          "id": "https://www.grid.ac/institutes/grid.49606.3d", 
          "name": [
            "Department of Microbiology and Institute of Biomedical Science, Hanyang University College of Medicine, Seoul, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Jung Mogg", 
        "id": "sg:person.01064772272.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064772272.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seoul National University", 
          "id": "https://www.grid.ac/institutes/grid.31501.36", 
          "name": [
            "Department of Internal Medicine, Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cha", 
        "givenName": "Mi Yeon", 
        "id": "sg:person.0634535604.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634535604.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seoul National University", 
          "id": "https://www.grid.ac/institutes/grid.31501.36", 
          "name": [
            "Department of Internal Medicine, Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jung", 
        "givenName": "Hyun Chae", 
        "id": "sg:person.01230372236.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230372236.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seoul National University", 
          "id": "https://www.grid.ac/institutes/grid.31501.36", 
          "name": [
            "Department of Internal Medicine, Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Song", 
        "givenName": "In Sung", 
        "id": "sg:person.01001342362.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001342362.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seoul National University", 
          "id": "https://www.grid.ac/institutes/grid.31501.36", 
          "name": [
            "Department of Internal Medicine, Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Joo Sung", 
        "id": "sg:person.07514633567.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07514633567.21"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/ijc.10901", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000941606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1517/13543784.13.10.1327", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005142023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/gut.42.6.779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010048197"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.intimp.2006.10.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016670231"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3181/0707-rm-186", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017225364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3181/0707-rm-186", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017225364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002800000187", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019766875", 
          "https://doi.org/10.1007/s002800000187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnci/dji377", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020681188"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnci/95.4.291", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020926707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.2010.54", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028780771", 
          "https://doi.org/10.1038/labinvest.2010.54"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1078-0432.ccr-0833-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029077459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(03)00521-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029513979"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ccr.2004.09.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031620015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2004.07.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032617810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m303860200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035031476"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0959-8049(02)00192-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035563718"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0959-8049(02)00192-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035563718"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1204535", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036775787", 
          "https://doi.org/10.1038/sj.onc.1204535"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1204535", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036775787", 
          "https://doi.org/10.1038/sj.onc.1204535"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1207230", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039161621", 
          "https://doi.org/10.1038/sj.onc.1207230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1207230", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039161621", 
          "https://doi.org/10.1038/sj.onc.1207230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1207230", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039161621", 
          "https://doi.org/10.1038/sj.onc.1207230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ijc.23593", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039555092"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrd1279", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040919652", 
          "https://doi.org/10.1038/nrd1279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrd1279", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040919652", 
          "https://doi.org/10.1038/nrd1279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.mib.0000235830.94057.c6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046191918"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.mib.0000235830.94057.c6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046191918"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.gie.2006.12.065", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047958243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0395-260", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053359707", 
          "https://doi.org/10.1038/nm0395-260"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.177.8.5612", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077303905"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083134793", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083134794", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-11", 
    "datePublishedReg": "2012-11-01", 
    "description": "BACKGROUND: Deguelin, a naturally occurring rotenoid, is known to be an Akt inhibitor and to have an anti-tumor effect on several cancers.\nAIMS: This study was performed to elucidate the effect of deguelin on apoptotic pathways related to NF-\u03baB signaling in colon cancer cells and on the anti-tumor effect in colon cancer xenograft mice.\nMETHODS: We studied COLO 205 and HCT116 cells in the presence or absence of deguelin. NF-\u03baB signaling was examined by real-time RT-PCR for interleukin (IL)-8, by Western blotting for I\u03baB phosphorylation/degradation, and by the electrophoretic mobility shift assay. Cell death was determined by the MTT assay, and apoptosis by Annexin V-FITC staining and caspase-3 activity. We also assessed the expression of antiapoptotic and proapoptotic factors by use of RT-PCR. In colon cancer xenograft mice, we evaluated the effect of deguelin on inoculated tumor growth, and apoptotic index was measured by the in vivo TUNEL assay.\nRESULTS: Deguelin significantly inhibited IL-8 gene expression, I\u03baB phosphorylation/degradation, and DNA binding activity of NF-\u03baB in colon cancer cells. Deguelin induced cell death and apoptosis in colon cancer cells in a dose and time-dependent manner. Deguelin down-regulated expression of NF-\u03baB-mediated antiapoptotic factors such as cFLIP, Bcl-2, and Bcl-X(L). In the colon cancer xenograft model, the volume of the tumor treated with deguelin was significantly lower than that of the control, and the apoptotic index for deguelin-treated mice was much higher.\nCONCLUSION: Deguelin might be a potential therapeutic agent for treatment of colorectal cancer.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10620-012-2237-x", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018525", 
        "issn": [
          "0163-2116", 
          "1573-2568"
        ], 
        "name": "Digestive Diseases and Sciences", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "11", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "57"
      }
    ], 
    "name": "Deguelin, an Akt Inhibitor, Down-Regulates NF-\u03baB Signaling and Induces Apoptosis in Colon Cancer Cells and Inhibits Tumor Growth in Mice", 
    "pagination": "2873-2882", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d371322c1741bc3b393e88d2b6ac44af565e65a62f0b0daa559ad10601199eac"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22623043"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7902782"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10620-012-2237-x"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1044389101"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10620-012-2237-x", 
      "https://app.dimensions.ai/details/publication/pub.1044389101"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000490.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s10620-012-2237-x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10620-012-2237-x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10620-012-2237-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10620-012-2237-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10620-012-2237-x'


 

This table displays all metadata directly associated to this object as RDF triples.

261 TRIPLES      21 PREDICATES      72 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10620-012-2237-x schema:about N04241d0680ee42cdb47664aaacbe0507
2 N0a45ca4bcabf4d0bbca851443b716372
3 N130f7e7905d04ae4b9b38f0d0a94e9ce
4 N17c74929f3194fdca0380242ded8dc61
5 N293368551c9d4eefa1d19ad56cc49fc6
6 N412f38e9579c41f19f4f9261e674270f
7 N4bb55519da0f4a7ab6f4c978b158da61
8 N55c6252466cc4f429a1bd8bc38fbedb1
9 N66fecfccfc214ec7837cd0e5bfca7047
10 N75370e51534d4da08c69be1401555afc
11 N7bb286dd535a4363a5765ffe8b0f8999
12 N7c4c779b981f4076a1a6b14f89022539
13 N7f60125753464e2c95dafbfd745c0bca
14 N8bce54de671942e99f0eb13dd8f9df96
15 N909d032bc6a54029a6450610c27ed367
16 N94ad6aa9989343f581b4e64f6cbc6148
17 N9bc29e0b194c424c8aecdf6e7ef6bb94
18 Ncd4d4e8d57414265a161312ef85418d0
19 anzsrc-for:11
20 anzsrc-for:1112
21 schema:author N342a7c485c5049b3a8e30d13a246a190
22 schema:citation sg:pub.10.1007/s002800000187
23 sg:pub.10.1038/labinvest.2010.54
24 sg:pub.10.1038/nm0395-260
25 sg:pub.10.1038/nrd1279
26 sg:pub.10.1038/sj.onc.1204535
27 sg:pub.10.1038/sj.onc.1207230
28 https://app.dimensions.ai/details/publication/pub.1083134793
29 https://app.dimensions.ai/details/publication/pub.1083134794
30 https://doi.org/10.1002/ijc.10901
31 https://doi.org/10.1002/ijc.23593
32 https://doi.org/10.1016/j.ccr.2004.09.021
33 https://doi.org/10.1016/j.cell.2004.07.013
34 https://doi.org/10.1016/j.gie.2006.12.065
35 https://doi.org/10.1016/j.intimp.2006.10.013
36 https://doi.org/10.1016/s0092-8674(03)00521-x
37 https://doi.org/10.1016/s0959-8049(02)00192-2
38 https://doi.org/10.1074/jbc.m303860200
39 https://doi.org/10.1093/jnci/95.4.291
40 https://doi.org/10.1093/jnci/dji377
41 https://doi.org/10.1097/01.mib.0000235830.94057.c6
42 https://doi.org/10.1136/gut.42.6.779
43 https://doi.org/10.1158/1078-0432.ccr-0833-3
44 https://doi.org/10.1517/13543784.13.10.1327
45 https://doi.org/10.3181/0707-rm-186
46 https://doi.org/10.4049/jimmunol.177.8.5612
47 schema:datePublished 2012-11
48 schema:datePublishedReg 2012-11-01
49 schema:description BACKGROUND: Deguelin, a naturally occurring rotenoid, is known to be an Akt inhibitor and to have an anti-tumor effect on several cancers. AIMS: This study was performed to elucidate the effect of deguelin on apoptotic pathways related to NF-κB signaling in colon cancer cells and on the anti-tumor effect in colon cancer xenograft mice. METHODS: We studied COLO 205 and HCT116 cells in the presence or absence of deguelin. NF-κB signaling was examined by real-time RT-PCR for interleukin (IL)-8, by Western blotting for IκB phosphorylation/degradation, and by the electrophoretic mobility shift assay. Cell death was determined by the MTT assay, and apoptosis by Annexin V-FITC staining and caspase-3 activity. We also assessed the expression of antiapoptotic and proapoptotic factors by use of RT-PCR. In colon cancer xenograft mice, we evaluated the effect of deguelin on inoculated tumor growth, and apoptotic index was measured by the in vivo TUNEL assay. RESULTS: Deguelin significantly inhibited IL-8 gene expression, IκB phosphorylation/degradation, and DNA binding activity of NF-κB in colon cancer cells. Deguelin induced cell death and apoptosis in colon cancer cells in a dose and time-dependent manner. Deguelin down-regulated expression of NF-κB-mediated antiapoptotic factors such as cFLIP, Bcl-2, and Bcl-X(L). In the colon cancer xenograft model, the volume of the tumor treated with deguelin was significantly lower than that of the control, and the apoptotic index for deguelin-treated mice was much higher. CONCLUSION: Deguelin might be a potential therapeutic agent for treatment of colorectal cancer.
50 schema:genre research_article
51 schema:inLanguage en
52 schema:isAccessibleForFree false
53 schema:isPartOf N3deea1054470486bb1aa1410830e0003
54 Ne8ebbff1665541759e37eceea6373ca7
55 sg:journal.1018525
56 schema:name Deguelin, an Akt Inhibitor, Down-Regulates NF-κB Signaling and Induces Apoptosis in Colon Cancer Cells and Inhibits Tumor Growth in Mice
57 schema:pagination 2873-2882
58 schema:productId N043d2f2af6b549d2b35291c4f2481938
59 N15107ff3d75b4d50b07e1f759967901f
60 N5952dd219dcd48429a7716ffb9af6491
61 N789d14b9e288428eb8aa9da20b4c6ad7
62 Nd7d6f3fd87d549cf8e778b0e4e9709cd
63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044389101
64 https://doi.org/10.1007/s10620-012-2237-x
65 schema:sdDatePublished 2019-04-10T14:04
66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
67 schema:sdPublisher Na668f1084c254da981561f654c80daf6
68 schema:url http://link.springer.com/10.1007/s10620-012-2237-x
69 sgo:license sg:explorer/license/
70 sgo:sdDataset articles
71 rdf:type schema:ScholarlyArticle
72 N04241d0680ee42cdb47664aaacbe0507 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name Animals
74 rdf:type schema:DefinedTerm
75 N043d2f2af6b549d2b35291c4f2481938 schema:name dimensions_id
76 schema:value pub.1044389101
77 rdf:type schema:PropertyValue
78 N0a45ca4bcabf4d0bbca851443b716372 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Down-Regulation
80 rdf:type schema:DefinedTerm
81 N130f7e7905d04ae4b9b38f0d0a94e9ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name CASP8 and FADD-Like Apoptosis Regulating Protein
83 rdf:type schema:DefinedTerm
84 N15107ff3d75b4d50b07e1f759967901f schema:name nlm_unique_id
85 schema:value 7902782
86 rdf:type schema:PropertyValue
87 N17c74929f3194fdca0380242ded8dc61 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Apoptosis
89 rdf:type schema:DefinedTerm
90 N293368551c9d4eefa1d19ad56cc49fc6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Blotting, Western
92 rdf:type schema:DefinedTerm
93 N342a7c485c5049b3a8e30d13a246a190 rdf:first sg:person.01312026504.84
94 rdf:rest N35d88e46901a4a23a8054a8757fc4dd7
95 N35d88e46901a4a23a8054a8757fc4dd7 rdf:first sg:person.01064772272.15
96 rdf:rest N8a884f8882de4743b31b90ebaa601a32
97 N3deea1054470486bb1aa1410830e0003 schema:volumeNumber 57
98 rdf:type schema:PublicationVolume
99 N412f38e9579c41f19f4f9261e674270f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Colonic Neoplasms
101 rdf:type schema:DefinedTerm
102 N4473b03ac7254af28e88fb5ba3e808da rdf:first sg:person.01230372236.08
103 rdf:rest N7738f80ebd604d90b392927d64925633
104 N4bb55519da0f4a7ab6f4c978b158da61 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Signal Transduction
106 rdf:type schema:DefinedTerm
107 N55c6252466cc4f429a1bd8bc38fbedb1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Cell Line, Tumor
109 rdf:type schema:DefinedTerm
110 N5952dd219dcd48429a7716ffb9af6491 schema:name readcube_id
111 schema:value d371322c1741bc3b393e88d2b6ac44af565e65a62f0b0daa559ad10601199eac
112 rdf:type schema:PropertyValue
113 N66fecfccfc214ec7837cd0e5bfca7047 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Mice
115 rdf:type schema:DefinedTerm
116 N75370e51534d4da08c69be1401555afc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Real-Time Polymerase Chain Reaction
118 rdf:type schema:DefinedTerm
119 N7738f80ebd604d90b392927d64925633 rdf:first sg:person.01001342362.12
120 rdf:rest N998ed8456d994f9988e64498bf7ca3a4
121 N789d14b9e288428eb8aa9da20b4c6ad7 schema:name pubmed_id
122 schema:value 22623043
123 rdf:type schema:PropertyValue
124 N7bb286dd535a4363a5765ffe8b0f8999 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name In Situ Nick-End Labeling
126 rdf:type schema:DefinedTerm
127 N7c4c779b981f4076a1a6b14f89022539 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Caspase 3
129 rdf:type schema:DefinedTerm
130 N7f60125753464e2c95dafbfd745c0bca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Statistics, Nonparametric
132 rdf:type schema:DefinedTerm
133 N8a884f8882de4743b31b90ebaa601a32 rdf:first sg:person.0634535604.12
134 rdf:rest N4473b03ac7254af28e88fb5ba3e808da
135 N8bce54de671942e99f0eb13dd8f9df96 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Proto-Oncogene Proteins c-bcl-2
137 rdf:type schema:DefinedTerm
138 N909d032bc6a54029a6450610c27ed367 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name NF-kappa B
140 rdf:type schema:DefinedTerm
141 N94ad6aa9989343f581b4e64f6cbc6148 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Interleukin-8
143 rdf:type schema:DefinedTerm
144 N998ed8456d994f9988e64498bf7ca3a4 rdf:first sg:person.07514633567.21
145 rdf:rest rdf:nil
146 N9bc29e0b194c424c8aecdf6e7ef6bb94 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name bcl-X Protein
148 rdf:type schema:DefinedTerm
149 Na668f1084c254da981561f654c80daf6 schema:name Springer Nature - SN SciGraph project
150 rdf:type schema:Organization
151 Ncd4d4e8d57414265a161312ef85418d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Rotenone
153 rdf:type schema:DefinedTerm
154 Nd7d6f3fd87d549cf8e778b0e4e9709cd schema:name doi
155 schema:value 10.1007/s10620-012-2237-x
156 rdf:type schema:PropertyValue
157 Ne8ebbff1665541759e37eceea6373ca7 schema:issueNumber 11
158 rdf:type schema:PublicationIssue
159 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
160 schema:name Medical and Health Sciences
161 rdf:type schema:DefinedTerm
162 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
163 schema:name Oncology and Carcinogenesis
164 rdf:type schema:DefinedTerm
165 sg:journal.1018525 schema:issn 0163-2116
166 1573-2568
167 schema:name Digestive Diseases and Sciences
168 rdf:type schema:Periodical
169 sg:person.01001342362.12 schema:affiliation https://www.grid.ac/institutes/grid.31501.36
170 schema:familyName Song
171 schema:givenName In Sung
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001342362.12
173 rdf:type schema:Person
174 sg:person.01064772272.15 schema:affiliation https://www.grid.ac/institutes/grid.49606.3d
175 schema:familyName Kim
176 schema:givenName Jung Mogg
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064772272.15
178 rdf:type schema:Person
179 sg:person.01230372236.08 schema:affiliation https://www.grid.ac/institutes/grid.31501.36
180 schema:familyName Jung
181 schema:givenName Hyun Chae
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230372236.08
183 rdf:type schema:Person
184 sg:person.01312026504.84 schema:affiliation https://www.grid.ac/institutes/grid.470090.a
185 schema:familyName Kang
186 schema:givenName Hyoun Woo
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312026504.84
188 rdf:type schema:Person
189 sg:person.0634535604.12 schema:affiliation https://www.grid.ac/institutes/grid.31501.36
190 schema:familyName Cha
191 schema:givenName Mi Yeon
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634535604.12
193 rdf:type schema:Person
194 sg:person.07514633567.21 schema:affiliation https://www.grid.ac/institutes/grid.31501.36
195 schema:familyName Kim
196 schema:givenName Joo Sung
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07514633567.21
198 rdf:type schema:Person
199 sg:pub.10.1007/s002800000187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019766875
200 https://doi.org/10.1007/s002800000187
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/labinvest.2010.54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028780771
203 https://doi.org/10.1038/labinvest.2010.54
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/nm0395-260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053359707
206 https://doi.org/10.1038/nm0395-260
207 rdf:type schema:CreativeWork
208 sg:pub.10.1038/nrd1279 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040919652
209 https://doi.org/10.1038/nrd1279
210 rdf:type schema:CreativeWork
211 sg:pub.10.1038/sj.onc.1204535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036775787
212 https://doi.org/10.1038/sj.onc.1204535
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/sj.onc.1207230 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039161621
215 https://doi.org/10.1038/sj.onc.1207230
216 rdf:type schema:CreativeWork
217 https://app.dimensions.ai/details/publication/pub.1083134793 schema:CreativeWork
218 https://app.dimensions.ai/details/publication/pub.1083134794 schema:CreativeWork
219 https://doi.org/10.1002/ijc.10901 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000941606
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1002/ijc.23593 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039555092
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/j.ccr.2004.09.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031620015
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/j.cell.2004.07.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032617810
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/j.gie.2006.12.065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047958243
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/j.intimp.2006.10.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016670231
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1016/s0092-8674(03)00521-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1029513979
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/s0959-8049(02)00192-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035563718
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1074/jbc.m303860200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035031476
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1093/jnci/95.4.291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020926707
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1093/jnci/dji377 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020681188
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1097/01.mib.0000235830.94057.c6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046191918
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1136/gut.42.6.779 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010048197
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1158/1078-0432.ccr-0833-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029077459
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1517/13543784.13.10.1327 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005142023
248 rdf:type schema:CreativeWork
249 https://doi.org/10.3181/0707-rm-186 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017225364
250 rdf:type schema:CreativeWork
251 https://doi.org/10.4049/jimmunol.177.8.5612 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077303905
252 rdf:type schema:CreativeWork
253 https://www.grid.ac/institutes/grid.31501.36 schema:alternateName Seoul National University
254 schema:name Department of Internal Medicine, Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, South Korea
255 rdf:type schema:Organization
256 https://www.grid.ac/institutes/grid.470090.a schema:alternateName Dongguk University Ilsan Hospital
257 schema:name Department of Internal Medicine, Dongguk University Ilsan Hospital, Seoul, Korea
258 rdf:type schema:Organization
259 https://www.grid.ac/institutes/grid.49606.3d schema:alternateName Hanyang University
260 schema:name Department of Microbiology and Institute of Biomedical Science, Hanyang University College of Medicine, Seoul, Korea
261 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...