In Vitro and In Vivo Protection of Stellate Cells from Apoptosis by Leptin View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-10

AUTHORS

Amir Qamar, Shehzad Zafar Sheikh, Ali Masud, Muhammad Nauman Jhandier, Irteza Bin Inayat, Wyel Hakim, Wajahat Zafar Mehal

ABSTRACT

Hepatic fibrogenesis is reduced in the absence of leptin. We hypothesized that leptin protects hepatic stellate cells (HSCs) from apoptosis and tested this in in vitro and in vivo systems. (i) Fas ligand (fas-L)-mediated apoptosis was induced in vitro in activated HSCs in the absence and presence of leptin. (ii) HSC apoptosis was also induced by UV irradiation in the absence and presence of leptin. (iii) Fas-L-mediated apoptosis was induced in vitro in HSCs from db/db mice in the absence and presence of leptin. (iv) Liver fibrosis was induced in wt and db/db mice. (v) Liver fibrosis was induced in wild-type mice with TAA, and mice received additional leptin or a control solution. HSC apoptosis was assessed by TUNEL staining. Western blot for alpha-SMA was used to determine differences in HSC activation. Results were as follows. (i) Fas-L induced significant apoptosis of HSC, and preincubation with leptin reduced this approximately threefold. (ii) Leptin provided no protection from UV-induced apoptosis. (iii) HSCs from db/db mice were not protected by leptin against fas-L-induced apoptosis. (iv) TAA-induced fibrosis was significantly less in db/db mice compared to wild type. (v) Wild-type mice receiving leptin had less apoptosis and more alpha-SMA than controls. We conclude that leptin protects HSC from in vitro and in vivo apoptosis. The antiapoptotic effect of leptin requires the long form of the leptin receptor and interacts with the apoptotic pathway proximal to mitochondrial activation. More... »

PAGES

1697-1705

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10620-006-9244-8

DOI

http://dx.doi.org/10.1007/s10620-006-9244-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004570399

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16957995


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fas Ligand Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepatocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leptin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liver", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred C57BL", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Cell Surface", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Leptin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thioacetamide", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Yale University", 
          "id": "https://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Section of Digestive Diseases, Yale University School of Medicine, 06520, New Haven, Connecticut, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Qamar", 
        "givenName": "Amir", 
        "id": "sg:person.0757112722.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757112722.65"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yale University", 
          "id": "https://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Section of Digestive Diseases, Yale University School of Medicine, 06520, New Haven, Connecticut, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sheikh", 
        "givenName": "Shehzad Zafar", 
        "id": "sg:person.01237437315.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237437315.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yale University", 
          "id": "https://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Section of Immunobiology, Yale University School of Medicine, 06520, New Haven, Connecticut, USA", 
            "Section of Pediatrics, Yale University School of Medicine, 06520, New Haven, Connecticut, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Masud", 
        "givenName": "Ali", 
        "id": "sg:person.01216426315.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216426315.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yale University", 
          "id": "https://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Section of Digestive Diseases, Yale University School of Medicine, 06520, New Haven, Connecticut, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jhandier", 
        "givenName": "Muhammad Nauman", 
        "id": "sg:person.01335234156.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335234156.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yale University", 
          "id": "https://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Section of Digestive Diseases, Yale University School of Medicine, 06520, New Haven, Connecticut, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Inayat", 
        "givenName": "Irteza Bin", 
        "id": "sg:person.011753424173.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011753424173.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yale University", 
          "id": "https://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Section of Digestive Diseases, Yale University School of Medicine, 06520, New Haven, Connecticut, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hakim", 
        "givenName": "Wyel", 
        "id": "sg:person.0610047261.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610047261.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yale University", 
          "id": "https://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Section of Digestive Diseases, Yale University School of Medicine, 06520, New Haven, Connecticut, USA", 
            "Section of Immunobiology, Yale University School of Medicine, 06520, New Haven, Connecticut, USA", 
            "Section of Digestive Diseases, Yale University, 333 Cedar Street, 1080 LMP, 06520-8019, New Haven, Connecticut, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mehal", 
        "givenName": "Wajahat Zafar", 
        "id": "sg:person.01340746246.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340746246.23"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1053/jhep.2002.33684", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003457449"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35037710", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003551467", 
          "https://doi.org/10.1038/35037710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35037710", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003551467", 
          "https://doi.org/10.1038/35037710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/gast.2002.33573", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005418184"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/gast.2002.32995", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009829970"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.ijo.0800437", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013295832", 
          "https://doi.org/10.1038/sj.ijo.0800437"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.ijo.0800437", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013295832", 
          "https://doi.org/10.1038/sj.ijo.0800437"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/gast.2002.36572", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013896923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1097/01.lab.0000069036.63405.5c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014106683", 
          "https://doi.org/10.1097/01.lab.0000069036.63405.5c"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/jhep.2002.34359", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021403450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/j.1572-0241.2003.07450.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023658025", 
          "https://doi.org/10.1111/j.1572-0241.2003.07450.x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0302-274", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024488928", 
          "https://doi.org/10.1038/nm0302-274"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0302-274", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024488928", 
          "https://doi.org/10.1038/nm0302-274"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0898-6568(02)00006-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024658715"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m207728200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029100396"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2249.2003.02190.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034782715"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1034/j.1600-0781.2000.160501.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037895773"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/hep.510290601", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037900078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-8278(02)00209-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039276670"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-8278(02)00209-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039276670"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.04-1847fje", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043080612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/hep.20051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044122339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0024-3205(03)00653-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047633669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0024-3205(03)00653-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047633669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci1018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048870214"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/hep.1840380432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051503870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/jhep.2002.32029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052616717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.168.4.1831", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074990993"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083169006", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fasebj.12.1.57", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083200964"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-10", 
    "datePublishedReg": "2006-10-01", 
    "description": "Hepatic fibrogenesis is reduced in the absence of leptin. We hypothesized that leptin protects hepatic stellate cells (HSCs) from apoptosis and tested this in in vitro and in vivo systems. (i) Fas ligand (fas-L)-mediated apoptosis was induced in vitro in activated HSCs in the absence and presence of leptin. (ii) HSC apoptosis was also induced by UV irradiation in the absence and presence of leptin. (iii) Fas-L-mediated apoptosis was induced in vitro in HSCs from db/db mice in the absence and presence of leptin. (iv) Liver fibrosis was induced in wt and db/db mice. (v) Liver fibrosis was induced in wild-type mice with TAA, and mice received additional leptin or a control solution. HSC apoptosis was assessed by TUNEL staining. Western blot for alpha-SMA was used to determine differences in HSC activation. Results were as follows. (i) Fas-L induced significant apoptosis of HSC, and preincubation with leptin reduced this approximately threefold. (ii) Leptin provided no protection from UV-induced apoptosis. (iii) HSCs from db/db mice were not protected by leptin against fas-L-induced apoptosis. (iv) TAA-induced fibrosis was significantly less in db/db mice compared to wild type. (v) Wild-type mice receiving leptin had less apoptosis and more alpha-SMA than controls. We conclude that leptin protects HSC from in vitro and in vivo apoptosis. The antiapoptotic effect of leptin requires the long form of the leptin receptor and interacts with the apoptotic pathway proximal to mitochondrial activation.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10620-006-9244-8", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2683185", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2438990", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2412031", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1018525", 
        "issn": [
          "0163-2116", 
          "1573-2568"
        ], 
        "name": "Digestive Diseases and Sciences", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "51"
      }
    ], 
    "name": "In Vitro and In Vivo Protection of Stellate Cells from Apoptosis by Leptin", 
    "pagination": "1697-1705", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10620-006-9244-8"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "15c75a3943a2d38e6a10dc44ef5d7eb468c232964653d576c9498fd2d1bdcf45"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1004570399"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7902782"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16957995"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10620-006-9244-8", 
      "https://app.dimensions.ai/details/publication/pub.1004570399"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T09:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000376_0000000376/records_56176_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s10620-006-9244-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10620-006-9244-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10620-006-9244-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10620-006-9244-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10620-006-9244-8'


 

This table displays all metadata directly associated to this object as RDF triples.

255 TRIPLES      21 PREDICATES      68 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10620-006-9244-8 schema:about N1a1019eb35ce4e0194264121307890b7
2 N1d0e88e681514c4594028b0ab0acafb6
3 N206621dd1e3e4de8a557afb581aa46be
4 N487dc1ddc07149a98b347cfbdaf5c1fa
5 N564bd8654bf44512816f9a6d074410e5
6 N708507607dde436889a583594c884d9c
7 N8b0fd407716d476893c275458622544f
8 Na62623ca11d246eaa9a2da39c90eb472
9 Nb918fea9e59d432ea4a81da163956da8
10 Ncafd2b8632504460b3b603c8787100b5
11 Ne9505f193e6148ee98716a702df53be3
12 Nefc24fef3c4d4c5cb9c20517a89eb7c1
13 Nf35de1d5ea6b4ffc92ddc5d786f2950a
14 Nf720fcee588f4c648de93f372da1ad80
15 anzsrc-for:06
16 anzsrc-for:0601
17 schema:author N41c8bbc255fd424587c2e847761d7d4d
18 schema:citation sg:pub.10.1038/35037710
19 sg:pub.10.1038/nm0302-274
20 sg:pub.10.1038/sj.ijo.0800437
21 sg:pub.10.1097/01.lab.0000069036.63405.5c
22 sg:pub.10.1111/j.1572-0241.2003.07450.x
23 https://app.dimensions.ai/details/publication/pub.1083169006
24 https://doi.org/10.1002/hep.1840380432
25 https://doi.org/10.1002/hep.20051
26 https://doi.org/10.1002/hep.510290601
27 https://doi.org/10.1016/s0024-3205(03)00653-2
28 https://doi.org/10.1016/s0168-8278(02)00209-x
29 https://doi.org/10.1016/s0898-6568(02)00006-2
30 https://doi.org/10.1034/j.1600-0781.2000.160501.x
31 https://doi.org/10.1046/j.1365-2249.2003.02190.x
32 https://doi.org/10.1053/gast.2002.32995
33 https://doi.org/10.1053/gast.2002.33573
34 https://doi.org/10.1053/gast.2002.36572
35 https://doi.org/10.1053/jhep.2002.32029
36 https://doi.org/10.1053/jhep.2002.33684
37 https://doi.org/10.1053/jhep.2002.34359
38 https://doi.org/10.1074/jbc.m207728200
39 https://doi.org/10.1096/fasebj.12.1.57
40 https://doi.org/10.1096/fj.04-1847fje
41 https://doi.org/10.1172/jci1018
42 https://doi.org/10.4049/jimmunol.168.4.1831
43 schema:datePublished 2006-10
44 schema:datePublishedReg 2006-10-01
45 schema:description Hepatic fibrogenesis is reduced in the absence of leptin. We hypothesized that leptin protects hepatic stellate cells (HSCs) from apoptosis and tested this in in vitro and in vivo systems. (i) Fas ligand (fas-L)-mediated apoptosis was induced in vitro in activated HSCs in the absence and presence of leptin. (ii) HSC apoptosis was also induced by UV irradiation in the absence and presence of leptin. (iii) Fas-L-mediated apoptosis was induced in vitro in HSCs from db/db mice in the absence and presence of leptin. (iv) Liver fibrosis was induced in wt and db/db mice. (v) Liver fibrosis was induced in wild-type mice with TAA, and mice received additional leptin or a control solution. HSC apoptosis was assessed by TUNEL staining. Western blot for alpha-SMA was used to determine differences in HSC activation. Results were as follows. (i) Fas-L induced significant apoptosis of HSC, and preincubation with leptin reduced this approximately threefold. (ii) Leptin provided no protection from UV-induced apoptosis. (iii) HSCs from db/db mice were not protected by leptin against fas-L-induced apoptosis. (iv) TAA-induced fibrosis was significantly less in db/db mice compared to wild type. (v) Wild-type mice receiving leptin had less apoptosis and more alpha-SMA than controls. We conclude that leptin protects HSC from in vitro and in vivo apoptosis. The antiapoptotic effect of leptin requires the long form of the leptin receptor and interacts with the apoptotic pathway proximal to mitochondrial activation.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree true
49 schema:isPartOf N25cb01001a7a4e24bf0692638ca1db5a
50 N5b2f3a07545c4c69861a24373772ef70
51 sg:journal.1018525
52 schema:name In Vitro and In Vivo Protection of Stellate Cells from Apoptosis by Leptin
53 schema:pagination 1697-1705
54 schema:productId N25cff1caece9491fa6e37f52d6e56eef
55 N315a603f761647adb843896fe67de064
56 N5cb8436c737c41f6828c3afe9c7ae67e
57 N84eee6bad0f84ceea7d9985a0f11f665
58 Nfa8de6b4644c4d5293c061731f1fde72
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004570399
60 https://doi.org/10.1007/s10620-006-9244-8
61 schema:sdDatePublished 2019-04-15T09:18
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher Neb70cdfe9a2e4c329e22716e8b33e2cf
64 schema:url http://link.springer.com/10.1007/s10620-006-9244-8
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N1a1019eb35ce4e0194264121307890b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Hepatocytes
70 rdf:type schema:DefinedTerm
71 N1c023041f6754fd99e4effc21dd65c0c rdf:first sg:person.0610047261.43
72 rdf:rest N77ace84501d2452a8d0ebc6dc1c3c82d
73 N1d0e88e681514c4594028b0ab0acafb6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Cell Culture Techniques
75 rdf:type schema:DefinedTerm
76 N206621dd1e3e4de8a557afb581aa46be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name Mice, Inbred C57BL
78 rdf:type schema:DefinedTerm
79 N25cb01001a7a4e24bf0692638ca1db5a schema:issueNumber 10
80 rdf:type schema:PublicationIssue
81 N25cff1caece9491fa6e37f52d6e56eef schema:name nlm_unique_id
82 schema:value 7902782
83 rdf:type schema:PropertyValue
84 N315a603f761647adb843896fe67de064 schema:name dimensions_id
85 schema:value pub.1004570399
86 rdf:type schema:PropertyValue
87 N41c8bbc255fd424587c2e847761d7d4d rdf:first sg:person.0757112722.65
88 rdf:rest N5e8d35798d8c46f59548009a88f3c07a
89 N487dc1ddc07149a98b347cfbdaf5c1fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Thioacetamide
91 rdf:type schema:DefinedTerm
92 N564bd8654bf44512816f9a6d074410e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Actins
94 rdf:type schema:DefinedTerm
95 N5b2f3a07545c4c69861a24373772ef70 schema:volumeNumber 51
96 rdf:type schema:PublicationVolume
97 N5cb8436c737c41f6828c3afe9c7ae67e schema:name pubmed_id
98 schema:value 16957995
99 rdf:type schema:PropertyValue
100 N5e8d35798d8c46f59548009a88f3c07a rdf:first sg:person.01237437315.24
101 rdf:rest Nba88a784b86542fa91352ad116c38d66
102 N708507607dde436889a583594c884d9c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Receptors, Leptin
104 rdf:type schema:DefinedTerm
105 N77ace84501d2452a8d0ebc6dc1c3c82d rdf:first sg:person.01340746246.23
106 rdf:rest rdf:nil
107 N84eee6bad0f84ceea7d9985a0f11f665 schema:name doi
108 schema:value 10.1007/s10620-006-9244-8
109 rdf:type schema:PropertyValue
110 N8b0fd407716d476893c275458622544f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Animals
112 rdf:type schema:DefinedTerm
113 N8f4f908d2e6b44f39de1e7ba8925ee40 rdf:first sg:person.011753424173.17
114 rdf:rest N1c023041f6754fd99e4effc21dd65c0c
115 N911406802d9d4165a283ada9990c4689 rdf:first sg:person.01335234156.39
116 rdf:rest N8f4f908d2e6b44f39de1e7ba8925ee40
117 Na62623ca11d246eaa9a2da39c90eb472 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Male
119 rdf:type schema:DefinedTerm
120 Nb918fea9e59d432ea4a81da163956da8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Leptin
122 rdf:type schema:DefinedTerm
123 Nba88a784b86542fa91352ad116c38d66 rdf:first sg:person.01216426315.52
124 rdf:rest N911406802d9d4165a283ada9990c4689
125 Ncafd2b8632504460b3b603c8787100b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Fas Ligand Protein
127 rdf:type schema:DefinedTerm
128 Ne9505f193e6148ee98716a702df53be3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Mice
130 rdf:type schema:DefinedTerm
131 Neb70cdfe9a2e4c329e22716e8b33e2cf schema:name Springer Nature - SN SciGraph project
132 rdf:type schema:Organization
133 Nefc24fef3c4d4c5cb9c20517a89eb7c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Receptors, Cell Surface
135 rdf:type schema:DefinedTerm
136 Nf35de1d5ea6b4ffc92ddc5d786f2950a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Liver
138 rdf:type schema:DefinedTerm
139 Nf720fcee588f4c648de93f372da1ad80 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Apoptosis
141 rdf:type schema:DefinedTerm
142 Nfa8de6b4644c4d5293c061731f1fde72 schema:name readcube_id
143 schema:value 15c75a3943a2d38e6a10dc44ef5d7eb468c232964653d576c9498fd2d1bdcf45
144 rdf:type schema:PropertyValue
145 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
146 schema:name Biological Sciences
147 rdf:type schema:DefinedTerm
148 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
149 schema:name Biochemistry and Cell Biology
150 rdf:type schema:DefinedTerm
151 sg:grant.2412031 http://pending.schema.org/fundedItem sg:pub.10.1007/s10620-006-9244-8
152 rdf:type schema:MonetaryGrant
153 sg:grant.2438990 http://pending.schema.org/fundedItem sg:pub.10.1007/s10620-006-9244-8
154 rdf:type schema:MonetaryGrant
155 sg:grant.2683185 http://pending.schema.org/fundedItem sg:pub.10.1007/s10620-006-9244-8
156 rdf:type schema:MonetaryGrant
157 sg:journal.1018525 schema:issn 0163-2116
158 1573-2568
159 schema:name Digestive Diseases and Sciences
160 rdf:type schema:Periodical
161 sg:person.011753424173.17 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
162 schema:familyName Inayat
163 schema:givenName Irteza Bin
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011753424173.17
165 rdf:type schema:Person
166 sg:person.01216426315.52 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
167 schema:familyName Masud
168 schema:givenName Ali
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216426315.52
170 rdf:type schema:Person
171 sg:person.01237437315.24 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
172 schema:familyName Sheikh
173 schema:givenName Shehzad Zafar
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237437315.24
175 rdf:type schema:Person
176 sg:person.01335234156.39 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
177 schema:familyName Jhandier
178 schema:givenName Muhammad Nauman
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335234156.39
180 rdf:type schema:Person
181 sg:person.01340746246.23 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
182 schema:familyName Mehal
183 schema:givenName Wajahat Zafar
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340746246.23
185 rdf:type schema:Person
186 sg:person.0610047261.43 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
187 schema:familyName Hakim
188 schema:givenName Wyel
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610047261.43
190 rdf:type schema:Person
191 sg:person.0757112722.65 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
192 schema:familyName Qamar
193 schema:givenName Amir
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757112722.65
195 rdf:type schema:Person
196 sg:pub.10.1038/35037710 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003551467
197 https://doi.org/10.1038/35037710
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/nm0302-274 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024488928
200 https://doi.org/10.1038/nm0302-274
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/sj.ijo.0800437 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013295832
203 https://doi.org/10.1038/sj.ijo.0800437
204 rdf:type schema:CreativeWork
205 sg:pub.10.1097/01.lab.0000069036.63405.5c schema:sameAs https://app.dimensions.ai/details/publication/pub.1014106683
206 https://doi.org/10.1097/01.lab.0000069036.63405.5c
207 rdf:type schema:CreativeWork
208 sg:pub.10.1111/j.1572-0241.2003.07450.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023658025
209 https://doi.org/10.1111/j.1572-0241.2003.07450.x
210 rdf:type schema:CreativeWork
211 https://app.dimensions.ai/details/publication/pub.1083169006 schema:CreativeWork
212 https://doi.org/10.1002/hep.1840380432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051503870
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1002/hep.20051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044122339
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1002/hep.510290601 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037900078
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/s0024-3205(03)00653-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047633669
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/s0168-8278(02)00209-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1039276670
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/s0898-6568(02)00006-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024658715
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1034/j.1600-0781.2000.160501.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037895773
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1046/j.1365-2249.2003.02190.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034782715
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1053/gast.2002.32995 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009829970
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1053/gast.2002.33573 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005418184
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1053/gast.2002.36572 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013896923
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1053/jhep.2002.32029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052616717
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1053/jhep.2002.33684 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003457449
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1053/jhep.2002.34359 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021403450
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1074/jbc.m207728200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029100396
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1096/fasebj.12.1.57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083200964
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1096/fj.04-1847fje schema:sameAs https://app.dimensions.ai/details/publication/pub.1043080612
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1172/jci1018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048870214
247 rdf:type schema:CreativeWork
248 https://doi.org/10.4049/jimmunol.168.4.1831 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074990993
249 rdf:type schema:CreativeWork
250 https://www.grid.ac/institutes/grid.47100.32 schema:alternateName Yale University
251 schema:name Section of Digestive Diseases, Yale University School of Medicine, 06520, New Haven, Connecticut, USA
252 Section of Digestive Diseases, Yale University, 333 Cedar Street, 1080 LMP, 06520-8019, New Haven, Connecticut, USA
253 Section of Immunobiology, Yale University School of Medicine, 06520, New Haven, Connecticut, USA
254 Section of Pediatrics, Yale University School of Medicine, 06520, New Haven, Connecticut, USA
255 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...