miR-200b-3p alleviates TNF-α-induced apoptosis and inflammation of intestinal epithelial cells and ulcerative colitis progression in rats via negatively regulating KHDRBS1 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2021-09-11

AUTHORS

Chunju Zheng, Ting Lu, Zhimin Fan

ABSTRACT

Ulcerative colitis (UC) is difficult to be treated. miRNAs are a group of gene regulators. Study demonstrated that miR-200b-3p is involved in the development of UC, but the specific molecular mechanism is still unclear. A UC model was established by injecting acetic acid into rectum of rats, which were then treated with miR-200b-3p antagonists and agonists. Weight change, fecal viscosity and fecal bleeding were measured to determine disease activity index. The ratio of colon length to weight was measured. Colon lesions were detected by H&E staining. ELISA was used to detect the expression of TGF-β in colon tissues and IL-10/CRP in serum. Intestinal epithelial cells (NCM460) were treated by TNF-α to create an inflammatory environment. MRNA and protein levels of miR-200b-3p, KHDRBS1, IL-10, IL-6, IL-1β, TGF-β, Bcl-2, Bax and C-capase-3 were detected by qRT-PCR and Western blot, respectively. TargetScan database and dual-luciferase reporter assay were conducted to predict the targeting relationship between miR-200b-3p and KHDRBS1. MTT and flow cytometry were respectively performed to detect cell proliferation and apoptosis. MiR-200b-3p expression was inhibited, leading to increased disease activity index and colonic length–weight ratio, and aggravation of lesions of the UC rat model. Up-regulation of miR-200b-3p can relieve inflammation and apoptosis of immune cells in UC rats. MiR-200b-3p targeted KHDRBS1 and inhibited its expression. Moreover, KHDRBS1 reversed the effects of miR-200b-3p on apoptosis, proliferation and inflammation of intestinal epithelial cells. MiR-200b-3p alleviates UC by negatively regulating KHDRBS1. More... »

PAGES

727-743

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10616-021-00490-3

DOI

http://dx.doi.org/10.1007/s10616-021-00490-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1141065952

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/34629748


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Anorectal, Huai\u2019an TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Huai\u2032an, China", 
          "id": "http://www.grid.ac/institutes/grid.410745.3", 
          "name": [
            "Department of Anorectal, Huai\u2019an TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Huai\u2032an, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zheng", 
        "givenName": "Chunju", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Anorectal, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, 157 Daming Road, Qinhuai District, 210001, Nanjing, Jiangsu, China", 
          "id": "http://www.grid.ac/institutes/grid.410745.3", 
          "name": [
            "Department of Anorectal, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, 157 Daming Road, Qinhuai District, 210001, Nanjing, Jiangsu, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lu", 
        "givenName": "Ting", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Anorectal, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, 157 Daming Road, Qinhuai District, 210001, Nanjing, Jiangsu, China", 
          "id": "http://www.grid.ac/institutes/grid.410745.3", 
          "name": [
            "Department of Anorectal, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, 157 Daming Road, Qinhuai District, 210001, Nanjing, Jiangsu, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fan", 
        "givenName": "Zhimin", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1245/s10434-009-0525-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006861804", 
          "https://doi.org/10.1245/s10434-009-0525-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature06005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013517631", 
          "https://doi.org/10.1038/nature06005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11373-006-9140-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029031099", 
          "https://doi.org/10.1007/s11373-006-9140-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10620-012-2417-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001239703", 
          "https://doi.org/10.1007/s10620-012-2417-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10209", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011250403", 
          "https://doi.org/10.1038/nature10209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10753-018-0809-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104139141", 
          "https://doi.org/10.1007/s10753-018-0809-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cddis.2013.22", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047416201", 
          "https://doi.org/10.1038/cddis.2013.22"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2021-09-11", 
    "datePublishedReg": "2021-09-11", 
    "description": "Ulcerative colitis (UC) is difficult to be treated. miRNAs are a group of gene regulators. Study demonstrated that miR-200b-3p is involved in the development of UC, but the specific molecular mechanism is still unclear. A UC model was established by injecting acetic acid into rectum of rats, which were then treated with miR-200b-3p antagonists and agonists. Weight change, fecal viscosity and fecal bleeding were measured to determine disease activity index. The ratio of colon length to weight was measured. Colon lesions were detected by H&E staining. ELISA was used to detect the expression of TGF-\u03b2 in colon tissues and IL-10/CRP in serum. Intestinal epithelial cells (NCM460) were treated by TNF-\u03b1 to create an inflammatory environment. MRNA and protein levels of miR-200b-3p, KHDRBS1, IL-10, IL-6, IL-1\u03b2, TGF-\u03b2, Bcl-2, Bax and C-capase-3 were detected by qRT-PCR and Western blot, respectively. TargetScan database and dual-luciferase reporter assay were conducted to predict the targeting relationship between miR-200b-3p and KHDRBS1. MTT and flow cytometry were respectively performed to detect cell proliferation and apoptosis. MiR-200b-3p expression was inhibited, leading to increased disease activity index and colonic length\u2013weight ratio, and aggravation of lesions of the UC rat model. Up-regulation of miR-200b-3p can relieve inflammation and apoptosis of immune cells in UC rats. MiR-200b-3p targeted KHDRBS1 and inhibited its expression. Moreover, KHDRBS1 reversed the effects of miR-200b-3p on apoptosis, proliferation and inflammation of intestinal epithelial cells. MiR-200b-3p alleviates UC by negatively regulating KHDRBS1.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s10616-021-00490-3", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1086307", 
        "issn": [
          "1381-5741", 
          "1573-0603"
        ], 
        "name": "Cytotechnology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "73"
      }
    ], 
    "keywords": [
      "Disease Activity Index", 
      "intestinal epithelial cells", 
      "ulcerative colitis", 
      "miR-200b", 
      "epithelial cells", 
      "development of UC", 
      "activity index", 
      "miR-200b-3p expression", 
      "expression of TGF", 
      "aggravation of lesions", 
      "UC rat model", 
      "dual-luciferase reporter", 
      "fecal bleeding", 
      "colitis progression", 
      "colon length", 
      "IL-10", 
      "rectum of rats", 
      "UC rats", 
      "IL-6", 
      "IL-1\u03b2", 
      "inflammatory environment", 
      "immune cells", 
      "rat model", 
      "colon lesions", 
      "colon tissues", 
      "flow cytometry", 
      "Western blot", 
      "inflammation", 
      "specific molecular mechanisms", 
      "capase-3", 
      "weight change", 
      "rats", 
      "protein levels", 
      "qRT-PCR", 
      "cell proliferation", 
      "Bcl-2", 
      "TargetScan database", 
      "TNF", 
      "lesions", 
      "apoptosis", 
      "TGF", 
      "fecal viscosity", 
      "molecular mechanisms", 
      "cells", 
      "proliferation", 
      "expression", 
      "colitis", 
      "bleeding", 
      "CRP", 
      "rectum", 
      "agonists", 
      "antagonist", 
      "UC model", 
      "ELISA", 
      "gene regulators", 
      "progression", 
      "serum", 
      "cytometry", 
      "aggravation", 
      "index", 
      "staining", 
      "blot", 
      "MTT", 
      "Bax", 
      "tissue", 
      "KHDRBS1", 
      "mRNA", 
      "alleviates", 
      "group", 
      "miRNAs", 
      "reporter", 
      "levels", 
      "weight", 
      "study", 
      "database", 
      "ratio", 
      "regulator", 
      "effect", 
      "acid", 
      "changes", 
      "mechanism", 
      "acetic acid", 
      "development", 
      "relationship", 
      "length-weight ratio", 
      "length", 
      "model", 
      "environment", 
      "viscosity", 
      "miR-200b-3p antagonists", 
      "IL-10/CRP", 
      "colonic length\u2013weight ratio", 
      "ulcerative colitis progression"
    ], 
    "name": "miR-200b-3p alleviates TNF-\u03b1-induced apoptosis and inflammation of intestinal epithelial cells and ulcerative colitis progression in rats via negatively regulating KHDRBS1", 
    "pagination": "727-743", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1141065952"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10616-021-00490-3"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "34629748"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10616-021-00490-3", 
      "https://app.dimensions.ai/details/publication/pub.1141065952"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_908.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s10616-021-00490-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10616-021-00490-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10616-021-00490-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10616-021-00490-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10616-021-00490-3'


 

This table displays all metadata directly associated to this object as RDF triples.

196 TRIPLES      22 PREDICATES      126 URIs      111 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10616-021-00490-3 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N04685ff8c8704b729de325c93fe26674
4 schema:citation sg:pub.10.1007/s10620-012-2417-8
5 sg:pub.10.1007/s10753-018-0809-4
6 sg:pub.10.1007/s11373-006-9140-4
7 sg:pub.10.1038/cddis.2013.22
8 sg:pub.10.1038/nature06005
9 sg:pub.10.1038/nature10209
10 sg:pub.10.1245/s10434-009-0525-2
11 schema:datePublished 2021-09-11
12 schema:datePublishedReg 2021-09-11
13 schema:description Ulcerative colitis (UC) is difficult to be treated. miRNAs are a group of gene regulators. Study demonstrated that miR-200b-3p is involved in the development of UC, but the specific molecular mechanism is still unclear. A UC model was established by injecting acetic acid into rectum of rats, which were then treated with miR-200b-3p antagonists and agonists. Weight change, fecal viscosity and fecal bleeding were measured to determine disease activity index. The ratio of colon length to weight was measured. Colon lesions were detected by H&E staining. ELISA was used to detect the expression of TGF-β in colon tissues and IL-10/CRP in serum. Intestinal epithelial cells (NCM460) were treated by TNF-α to create an inflammatory environment. MRNA and protein levels of miR-200b-3p, KHDRBS1, IL-10, IL-6, IL-1β, TGF-β, Bcl-2, Bax and C-capase-3 were detected by qRT-PCR and Western blot, respectively. TargetScan database and dual-luciferase reporter assay were conducted to predict the targeting relationship between miR-200b-3p and KHDRBS1. MTT and flow cytometry were respectively performed to detect cell proliferation and apoptosis. MiR-200b-3p expression was inhibited, leading to increased disease activity index and colonic length–weight ratio, and aggravation of lesions of the UC rat model. Up-regulation of miR-200b-3p can relieve inflammation and apoptosis of immune cells in UC rats. MiR-200b-3p targeted KHDRBS1 and inhibited its expression. Moreover, KHDRBS1 reversed the effects of miR-200b-3p on apoptosis, proliferation and inflammation of intestinal epithelial cells. MiR-200b-3p alleviates UC by negatively regulating KHDRBS1.
14 schema:genre article
15 schema:inLanguage en
16 schema:isAccessibleForFree false
17 schema:isPartOf Ne71d88bcb0cd41ba9a804f5dc2aeb87d
18 Nfed507f489ad40f9a54b143eb4da5241
19 sg:journal.1086307
20 schema:keywords Bax
21 Bcl-2
22 CRP
23 Disease Activity Index
24 ELISA
25 IL-10
26 IL-10/CRP
27 IL-1β
28 IL-6
29 KHDRBS1
30 MTT
31 TGF
32 TNF
33 TargetScan database
34 UC model
35 UC rat model
36 UC rats
37 Western blot
38 acetic acid
39 acid
40 activity index
41 aggravation
42 aggravation of lesions
43 agonists
44 alleviates
45 antagonist
46 apoptosis
47 bleeding
48 blot
49 capase-3
50 cell proliferation
51 cells
52 changes
53 colitis
54 colitis progression
55 colon length
56 colon lesions
57 colon tissues
58 colonic length–weight ratio
59 cytometry
60 database
61 development
62 development of UC
63 dual-luciferase reporter
64 effect
65 environment
66 epithelial cells
67 expression
68 expression of TGF
69 fecal bleeding
70 fecal viscosity
71 flow cytometry
72 gene regulators
73 group
74 immune cells
75 index
76 inflammation
77 inflammatory environment
78 intestinal epithelial cells
79 length
80 length-weight ratio
81 lesions
82 levels
83 mRNA
84 mechanism
85 miR-200b
86 miR-200b-3p antagonists
87 miR-200b-3p expression
88 miRNAs
89 model
90 molecular mechanisms
91 progression
92 proliferation
93 protein levels
94 qRT-PCR
95 rat model
96 ratio
97 rats
98 rectum
99 rectum of rats
100 regulator
101 relationship
102 reporter
103 serum
104 specific molecular mechanisms
105 staining
106 study
107 tissue
108 ulcerative colitis
109 ulcerative colitis progression
110 viscosity
111 weight
112 weight change
113 schema:name miR-200b-3p alleviates TNF-α-induced apoptosis and inflammation of intestinal epithelial cells and ulcerative colitis progression in rats via negatively regulating KHDRBS1
114 schema:pagination 727-743
115 schema:productId N334c78f269904659959c688b239f26df
116 Ndd90e67c0771461fa2b8423f38f4e23a
117 Ne557ac167cc14549a9d6e191a2e971cb
118 schema:sameAs https://app.dimensions.ai/details/publication/pub.1141065952
119 https://doi.org/10.1007/s10616-021-00490-3
120 schema:sdDatePublished 2022-01-01T18:59
121 schema:sdLicense https://scigraph.springernature.com/explorer/license/
122 schema:sdPublisher Nf0da46aaaeec4fd18539a84374fc5e06
123 schema:url https://doi.org/10.1007/s10616-021-00490-3
124 sgo:license sg:explorer/license/
125 sgo:sdDataset articles
126 rdf:type schema:ScholarlyArticle
127 N04685ff8c8704b729de325c93fe26674 rdf:first Nfdc8ac2991444ad9b2a758fa8bc3c0c6
128 rdf:rest N6524f9dc9fcf42a990ebfda8d71d0bed
129 N334c78f269904659959c688b239f26df schema:name doi
130 schema:value 10.1007/s10616-021-00490-3
131 rdf:type schema:PropertyValue
132 N6524f9dc9fcf42a990ebfda8d71d0bed rdf:first N6878e5d71a904a7ba111ee173bf3f6fb
133 rdf:rest Nb3fb11e56fd841b598e481d1fbb668b2
134 N6878e5d71a904a7ba111ee173bf3f6fb schema:affiliation grid-institutes:grid.410745.3
135 schema:familyName Lu
136 schema:givenName Ting
137 rdf:type schema:Person
138 N7b7ab37b45814bc4b14788c476088e64 schema:affiliation grid-institutes:grid.410745.3
139 schema:familyName Fan
140 schema:givenName Zhimin
141 rdf:type schema:Person
142 Nb3fb11e56fd841b598e481d1fbb668b2 rdf:first N7b7ab37b45814bc4b14788c476088e64
143 rdf:rest rdf:nil
144 Ndd90e67c0771461fa2b8423f38f4e23a schema:name dimensions_id
145 schema:value pub.1141065952
146 rdf:type schema:PropertyValue
147 Ne557ac167cc14549a9d6e191a2e971cb schema:name pubmed_id
148 schema:value 34629748
149 rdf:type schema:PropertyValue
150 Ne71d88bcb0cd41ba9a804f5dc2aeb87d schema:volumeNumber 73
151 rdf:type schema:PublicationVolume
152 Nf0da46aaaeec4fd18539a84374fc5e06 schema:name Springer Nature - SN SciGraph project
153 rdf:type schema:Organization
154 Nfdc8ac2991444ad9b2a758fa8bc3c0c6 schema:affiliation grid-institutes:grid.410745.3
155 schema:familyName Zheng
156 schema:givenName Chunju
157 rdf:type schema:Person
158 Nfed507f489ad40f9a54b143eb4da5241 schema:issueNumber 5
159 rdf:type schema:PublicationIssue
160 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
161 schema:name Biological Sciences
162 rdf:type schema:DefinedTerm
163 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
164 schema:name Biochemistry and Cell Biology
165 rdf:type schema:DefinedTerm
166 sg:journal.1086307 schema:issn 1381-5741
167 1573-0603
168 schema:name Cytotechnology
169 schema:publisher Springer Nature
170 rdf:type schema:Periodical
171 sg:pub.10.1007/s10620-012-2417-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001239703
172 https://doi.org/10.1007/s10620-012-2417-8
173 rdf:type schema:CreativeWork
174 sg:pub.10.1007/s10753-018-0809-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104139141
175 https://doi.org/10.1007/s10753-018-0809-4
176 rdf:type schema:CreativeWork
177 sg:pub.10.1007/s11373-006-9140-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029031099
178 https://doi.org/10.1007/s11373-006-9140-4
179 rdf:type schema:CreativeWork
180 sg:pub.10.1038/cddis.2013.22 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047416201
181 https://doi.org/10.1038/cddis.2013.22
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/nature06005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013517631
184 https://doi.org/10.1038/nature06005
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/nature10209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011250403
187 https://doi.org/10.1038/nature10209
188 rdf:type schema:CreativeWork
189 sg:pub.10.1245/s10434-009-0525-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006861804
190 https://doi.org/10.1245/s10434-009-0525-2
191 rdf:type schema:CreativeWork
192 grid-institutes:grid.410745.3 schema:alternateName Department of Anorectal, Huai’an TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Huai′an, China
193 Department of Anorectal, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, 157 Daming Road, Qinhuai District, 210001, Nanjing, Jiangsu, China
194 schema:name Department of Anorectal, Huai’an TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Huai′an, China
195 Department of Anorectal, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, 157 Daming Road, Qinhuai District, 210001, Nanjing, Jiangsu, China
196 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...