Involvement of increased expression of chemokine C–C motif chemokine 22 (CCL22)/CC chemokine receptor 4 (CCR4) in the inflammatory injury and ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2021-09-03

AUTHORS

Haiqiao Xu, Shibang Lin, Haizhou Huang

ABSTRACT

CCL22, which could induce chondrocyte apoptosis, was identified to be overexpressed in damaged cartilage. This study was conducted with the aim of investigating the effects of CCL22 interference on chondrocyte injury. The osteoarthritis model was established by stimulating chondrocytes with LPS. The expressions of CCL22, CCR4, matrix metallopeptidase (MMP) 3, MMP9, MMP13, (a disintegrin and metalloproteinase with thrombospondin-like motifs) ADAMTS-4, collagen II and inflammatory cytokines were measured using quantitative reverse transcription PCR (RT-qPCR) and western blot. Besides, immunoprecipitation (IP) was employed to verify the binding of CCL22 and CCR4. After CCR4 was overexpressed, cell viability was observed using Cell Counting Kit-8 (CCK-8). Additionally, cell apoptosis as well as its related proteins was detected by TUNEL and western blot, respectively. ng What’s more, glycosaminoglycan (GAG) level was detected using GAG kits. CCL22 and CCR4 expression increased noticeably in LPS-stimulated ATDC5 chondrocytes. CCL22 inhibition could suppress the expression of CCR4 in LPS-induced ATDC5 cells. Likewise, CCL22 inhibition could revive the activation of LPS-induced ATDC5 cells by regulating CCR4. In addition, CCL22 knockdown alleviated inflammatory response and cell apoptosis through CCR4. Furthermore, the cartilage degradation of ADTC5 cells could be relieved by CCL22 silence via regulating CCR4. CCL22/CCR4 expression was increased in osteoarthritic cartilage injury and participated in the inflammation and cartilage degradation of chondrocytes. More... »

PAGES

715-726

References to SciGraph publications

  • 2017-02-17. The minor collagens in articular cartilage in PROTEIN & CELL
  • 2019-03-20. Ageing and Osteoarthritis in BIOCHEMISTRY AND CELL BIOLOGY OF AGEING: PART II CLINICAL SCIENCE
  • 2014-12-23. Origin and function of cartilage stem/progenitor cells in osteoarthritis in NATURE REVIEWS RHEUMATOLOGY
  • 2013-01-11. Low Back Pain and Lumbar Spine Osteoarthritis: How Are They Related? in CURRENT RHEUMATOLOGY REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10616-021-00489-w

    DOI

    http://dx.doi.org/10.1007/s10616-021-00489-w

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1140869325

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/34629747


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