A simultaneous assessment metric for MAb quantity and glycan quality View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-10

AUTHORS

Gerald Drouillard, Gordon Hayward, Julie Vale, Roshni Dutton

ABSTRACT

As a critical quality attribute, glycosylation represents an important consideration when analyzing the success of a glycoprotein production process. Though critical, glycosylation is not the only measure of culture success; other factors, including culture size, maintenance, and productivity, are also critical. A new metric was developed to address both product quality, as measured through glycosylation, and product quantity, as measured through product concentration. A monoclonal antibody Chinese hamster ovary cell culture model system was used to assess this metric across various media formulations. In a model test system, the metric discriminated that some media supplements had a net positive impact on productivity and glycosylation, while others had a net negative impact on productivity and glycosylation. More... »

PAGES

1987-1997

Journal

TITLE

Cytotechnology

ISSUE

5

VOLUME

68

Author Affiliations

From Grant

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10616-016-0011-1

DOI

http://dx.doi.org/10.1007/s10616-016-0011-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004133719

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27502107


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