Agitation increases expansion of cord blood hematopoietic cells and promotes their differentiation into myeloid lineage View Full Text


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Article Info

DATE

2016-08

AUTHORS

Hasti Hosseinizand, Marzieh Ebrahimi, Mohammad J. Abdekhodaie

ABSTRACT

Mechanical stress caused by agitation is one of the factors that can affect hematopoietic stem cell expansion in suspension bioreactors. Therefore, we have investigated the effects of agitation on umbilical cord blood hematopoietic stem cell (UCB-HSC) growth and differentiation. A comparison was made between various agitation rates (20, 40 and 60 rpm) in spinner-flask and cells cultured in glass petri dish as a static culture. Moreover, the fluid dynamic at various agitation rates of spinner-flask was analyzed to determine shear stress. The spinner-flask contained a rotational moving mixer with glass ball and was kept in tissue culture incubator. To reduce consumption of cytokines, UCB-serum was used which widely decreased the costs. Our results determined that, agitation rate at 40 rpm promoted UCB-HSCs expansion and their colony forming potential. Myeloid progenitors were the main type of cells at 40 rpm agitation rate. The results of glucose consumption and lactic acid production were in complete agreement with colony assay and expansion data and indicated the superiority of culture in spinner-flask when agitated at 40 rpm over to other agitation speeds and also static culture. Cell viability and colony count was affected by changing the agitation speed. We assume that changes in cell growth resulted from the effect of shear stress directly on cell viability, and indirectly on signaling pathways that influence the cells to differentiate. More... »

PAGES

969-978

References to SciGraph publications

  • 2011-07. Process challenges relating to hematopoietic stem cell cultivation in bioreactors in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2012-11. A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cells in BIOTECHNOLOGY LETTERS
  • 2008-03. Expansion of human hematopoietic stem cells for transplantation: trends and perspectives in CYTOTECHNOLOGY
  • 1998-11. Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions in CYTOTECHNOLOGY
  • 2006-06. The stem-cell niche as an entity of action in NATURE
  • 2010-12. Optimization of ex vivo hematopoietic stem cell expansion in intermittent dynamic cultures in BIOTECHNOLOGY LETTERS
  • 2008-01. Hematopoietic reconstitution of CD34+ cells grown in static and stirred culture systems in NOD/SCID mice in BIOTECHNOLOGY LETTERS
  • 2003-01. Suspension culture of hematopoietic stem cells in stirred bioreactors in BIOTECHNOLOGY LETTERS
  • 1993-03. Expansion of Human Bone Marrow Progenitor Cells in a High Cell Density Continuous Perfusion System in BIO/TECHNOLOGY
  • 1992-07. Agitation effects on microcarrier and suspension CHO cells in BIOTECHNOLOGY LETTERS
  • 1994-09. Expansion of Hematopoietic Progenitor Cell Populations in Stirred Suspension Bioreactors of Normal Human Bone Marrow Cells in BIO/TECHNOLOGY
  • 2001-05. Ex vivo expansion of hematopoietic stem cells in bioreactors in BIOTECHNOLOGY LETTERS
  • 1999-07. Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells in CYTOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10616-015-9851-3

    DOI

    http://dx.doi.org/10.1007/s10616-015-9851-3

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26264594


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