Agitation increases expansion of cord blood hematopoietic cells and promotes their differentiation into myeloid lineage View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-08

AUTHORS

Hasti Hosseinizand, Marzieh Ebrahimi, Mohammad J. Abdekhodaie

ABSTRACT

Mechanical stress caused by agitation is one of the factors that can affect hematopoietic stem cell expansion in suspension bioreactors. Therefore, we have investigated the effects of agitation on umbilical cord blood hematopoietic stem cell (UCB-HSC) growth and differentiation. A comparison was made between various agitation rates (20, 40 and 60 rpm) in spinner-flask and cells cultured in glass petri dish as a static culture. Moreover, the fluid dynamic at various agitation rates of spinner-flask was analyzed to determine shear stress. The spinner-flask contained a rotational moving mixer with glass ball and was kept in tissue culture incubator. To reduce consumption of cytokines, UCB-serum was used which widely decreased the costs. Our results determined that, agitation rate at 40 rpm promoted UCB-HSCs expansion and their colony forming potential. Myeloid progenitors were the main type of cells at 40 rpm agitation rate. The results of glucose consumption and lactic acid production were in complete agreement with colony assay and expansion data and indicated the superiority of culture in spinner-flask when agitated at 40 rpm over to other agitation speeds and also static culture. Cell viability and colony count was affected by changing the agitation speed. We assume that changes in cell growth resulted from the effect of shear stress directly on cell viability, and indirectly on signaling pathways that influence the cells to differentiate. More... »

PAGES

969-978

References to SciGraph publications

  • 2011-07. Process challenges relating to hematopoietic stem cell cultivation in bioreactors in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2012-11. A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cells in BIOTECHNOLOGY LETTERS
  • 2008-03. Expansion of human hematopoietic stem cells for transplantation: trends and perspectives in CYTOTECHNOLOGY
  • 1998-11. Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions in CYTOTECHNOLOGY
  • 2006-06. The stem-cell niche as an entity of action in NATURE
  • 2010-12. Optimization of ex vivo hematopoietic stem cell expansion in intermittent dynamic cultures in BIOTECHNOLOGY LETTERS
  • 2008-01. Hematopoietic reconstitution of CD34+ cells grown in static and stirred culture systems in NOD/SCID mice in BIOTECHNOLOGY LETTERS
  • 2003-01. Suspension culture of hematopoietic stem cells in stirred bioreactors in BIOTECHNOLOGY LETTERS
  • 1993-03. Expansion of Human Bone Marrow Progenitor Cells in a High Cell Density Continuous Perfusion System in BIO/TECHNOLOGY
  • 1992-07. Agitation effects on microcarrier and suspension CHO cells in BIOTECHNOLOGY LETTERS
  • 1994-09. Expansion of Hematopoietic Progenitor Cell Populations in Stirred Suspension Bioreactors of Normal Human Bone Marrow Cells in BIO/TECHNOLOGY
  • 2001-05. Ex vivo expansion of hematopoietic stem cells in bioreactors in BIOTECHNOLOGY LETTERS
  • 1999-07. Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells in CYTOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10616-015-9851-3

    DOI

    http://dx.doi.org/10.1007/s10616-015-9851-3

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1045119257

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26264594


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Royan Institute", 
              "id": "https://www.grid.ac/institutes/grid.419336.a", 
              "name": [
                "Department of Chemical and Petroleum Engineering, Sharif University of Technology, 11155-9465, Tehran, Iran", 
                "Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hosseinizand", 
            "givenName": "Hasti", 
            "id": "sg:person.015101725225.65", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015101725225.65"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Royan Institute", 
              "id": "https://www.grid.ac/institutes/grid.419336.a", 
              "name": [
                "Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ebrahimi", 
            "givenName": "Marzieh", 
            "id": "sg:person.01161343145.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161343145.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sharif University of Technology", 
              "id": "https://www.grid.ac/institutes/grid.412553.4", 
              "name": [
                "Department of Chemical and Petroleum Engineering, Sharif University of Technology, 11155-9465, Tehran, Iran"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Abdekhodaie", 
            "givenName": "Mohammad J.", 
            "id": "sg:person.01036204527.48", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036204527.48"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nbt0393-368", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000504379", 
              "https://doi.org/10.1038/nbt0393-368"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10295-011-0951-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005308733", 
              "https://doi.org/10.1007/s10295-011-0951-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.20900", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008176312"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.20900", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008176312"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02439320", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008678274", 
              "https://doi.org/10.1007/bf02439320"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02439320", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008678274", 
              "https://doi.org/10.1007/bf02439320"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biotechadv.2011.06.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009358353"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(06)19018-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013050371"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jim.2010.05.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014874255"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bej.2006.11.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015058102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bp980032e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015092228"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1097-0290(20000620)68:6<697::aid-bit14>3.0.co;2-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017649614"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/btpr.582", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017650432"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0167-7799(03)00076-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018672050"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0167-7799(03)00076-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018672050"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-010-0355-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019987694", 
              "https://doi.org/10.1007/s10529-010-0355-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1008085932764", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020997857", 
              "https://doi.org/10.1023/a:1008085932764"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1097-0290(19980905)59:5<534::aid-bit2>3.0.co;2-b", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021766827"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0994-909", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022671474", 
              "https://doi.org/10.1038/nbt0994-909"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1056/nejm198910263211707", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023228454"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-012-0993-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024007931", 
              "https://doi.org/10.1007/s10529-012-0993-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jbiotec.2012.06.025", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028137709"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1010350215989", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032011100", 
              "https://doi.org/10.1023/a:1010350215989"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0141-0229(00)00346-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032164031"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1097-0290(20000720)69:2<171::aid-bit6>3.0.co;2-c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032935299"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4061/2011/620247", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033159527"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1021994026859", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033211656", 
              "https://doi.org/10.1023/a:1021994026859"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.10788", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037428194"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jbiotec.2006.01.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038276357"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1008042000744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038708051", 
              "https://doi.org/10.1023/a:1008042000744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.bioeng.1.1.129", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044257098"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0167-7799(91)90145-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044696037"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0167-7799(91)90145-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044696037"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0167-7799(00)01474-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045520262"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04957", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047165782", 
              "https://doi.org/10.1038/nature04957"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04957", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047165782", 
              "https://doi.org/10.1038/nature04957"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04957", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047165782", 
              "https://doi.org/10.1038/nature04957"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0141-0229(03)00144-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048278333"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0141-0229(03)00144-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048278333"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3109/21691401.2014.897630", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048598937"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-007-9517-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048694640", 
              "https://doi.org/10.1007/s10529-007-9517-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-007-9517-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048694640", 
              "https://doi.org/10.1007/s10529-007-9517-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3109/10731199.2012.712043", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048849211"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10616-008-9144-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052896273", 
              "https://doi.org/10.1007/s10616-008-9144-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/ten.tec.2013.0040", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059317649"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082861925", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2016-08", 
        "datePublishedReg": "2016-08-01", 
        "description": "Mechanical stress caused by agitation is one of the factors that can affect hematopoietic stem cell expansion in suspension bioreactors. Therefore, we have investigated the effects of agitation on umbilical cord blood hematopoietic stem cell (UCB-HSC) growth and differentiation. A comparison was made between various agitation rates (20, 40 and 60\u00a0rpm) in spinner-flask and cells cultured in glass petri dish as a static culture. Moreover, the fluid dynamic at various agitation rates of spinner-flask was analyzed to determine shear stress. The spinner-flask contained a rotational moving mixer with glass ball and was kept in tissue culture incubator. To reduce consumption of cytokines, UCB-serum was used which widely decreased the costs. Our results determined that, agitation rate at 40\u00a0rpm promoted UCB-HSCs expansion and their colony forming potential. Myeloid progenitors were the main type of cells at 40\u00a0rpm agitation rate. The results of glucose consumption and lactic acid production were in complete agreement with colony assay and expansion data and indicated the superiority of culture in spinner-flask when agitated at 40\u00a0rpm over to other agitation speeds and also static culture. Cell viability and colony count was affected by changing the agitation speed. We assume that changes in cell growth resulted from the effect of shear stress directly on cell viability, and indirectly on signaling pathways that influence the cells to differentiate. ", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s10616-015-9851-3", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1086307", 
            "issn": [
              "1381-5741", 
              "1573-0603"
            ], 
            "name": "Cytotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "68"
          }
        ], 
        "name": "Agitation increases expansion of cord blood hematopoietic cells and promotes their differentiation into myeloid lineage", 
        "pagination": "969-978", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "d6eb064d77d21090dcaa194ed6885b4ae6debcb3bd5def5a1506f671966fce0d"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "26264594"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "8807027"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s10616-015-9851-3"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1045119257"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s10616-015-9851-3", 
          "https://app.dimensions.ai/details/publication/pub.1045119257"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T18:21", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000515.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs10616-015-9851-3"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10616-015-9851-3'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10616-015-9851-3'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10616-015-9851-3'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10616-015-9851-3'


     

    This table displays all metadata directly associated to this object as RDF triples.

    213 TRIPLES      21 PREDICATES      67 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s10616-015-9851-3 schema:about anzsrc-for:06
    2 anzsrc-for:0601
    3 schema:author Nbe3c6d0175914485afe43c30d8d9ae1e
    4 schema:citation sg:pub.10.1007/bf02439320
    5 sg:pub.10.1007/s10295-011-0951-6
    6 sg:pub.10.1007/s10529-007-9517-0
    7 sg:pub.10.1007/s10529-010-0355-0
    8 sg:pub.10.1007/s10529-012-0993-5
    9 sg:pub.10.1007/s10616-008-9144-1
    10 sg:pub.10.1023/a:1008042000744
    11 sg:pub.10.1023/a:1008085932764
    12 sg:pub.10.1023/a:1010350215989
    13 sg:pub.10.1023/a:1021994026859
    14 sg:pub.10.1038/nature04957
    15 sg:pub.10.1038/nbt0393-368
    16 sg:pub.10.1038/nbt0994-909
    17 https://app.dimensions.ai/details/publication/pub.1082861925
    18 https://doi.org/10.1002/(sici)1097-0290(19980905)59:5<534::aid-bit2>3.0.co;2-b
    19 https://doi.org/10.1002/(sici)1097-0290(20000620)68:6<697::aid-bit14>3.0.co;2-6
    20 https://doi.org/10.1002/(sici)1097-0290(20000720)69:2<171::aid-bit6>3.0.co;2-c
    21 https://doi.org/10.1002/bit.10788
    22 https://doi.org/10.1002/bit.20900
    23 https://doi.org/10.1002/btpr.582
    24 https://doi.org/10.1016/0167-7799(91)90145-8
    25 https://doi.org/10.1016/j.bej.2006.11.024
    26 https://doi.org/10.1016/j.biotechadv.2011.06.009
    27 https://doi.org/10.1016/j.jbiotec.2006.01.020
    28 https://doi.org/10.1016/j.jbiotec.2012.06.025
    29 https://doi.org/10.1016/j.jim.2010.05.007
    30 https://doi.org/10.1016/s0076-6879(06)19018-7
    31 https://doi.org/10.1016/s0141-0229(00)00346-x
    32 https://doi.org/10.1016/s0141-0229(03)00144-3
    33 https://doi.org/10.1016/s0167-7799(00)01474-8
    34 https://doi.org/10.1016/s0167-7799(03)00076-3
    35 https://doi.org/10.1021/bp980032e
    36 https://doi.org/10.1056/nejm198910263211707
    37 https://doi.org/10.1089/ten.tec.2013.0040
    38 https://doi.org/10.1146/annurev.bioeng.1.1.129
    39 https://doi.org/10.3109/10731199.2012.712043
    40 https://doi.org/10.3109/21691401.2014.897630
    41 https://doi.org/10.4061/2011/620247
    42 schema:datePublished 2016-08
    43 schema:datePublishedReg 2016-08-01
    44 schema:description Mechanical stress caused by agitation is one of the factors that can affect hematopoietic stem cell expansion in suspension bioreactors. Therefore, we have investigated the effects of agitation on umbilical cord blood hematopoietic stem cell (UCB-HSC) growth and differentiation. A comparison was made between various agitation rates (20, 40 and 60 rpm) in spinner-flask and cells cultured in glass petri dish as a static culture. Moreover, the fluid dynamic at various agitation rates of spinner-flask was analyzed to determine shear stress. The spinner-flask contained a rotational moving mixer with glass ball and was kept in tissue culture incubator. To reduce consumption of cytokines, UCB-serum was used which widely decreased the costs. Our results determined that, agitation rate at 40 rpm promoted UCB-HSCs expansion and their colony forming potential. Myeloid progenitors were the main type of cells at 40 rpm agitation rate. The results of glucose consumption and lactic acid production were in complete agreement with colony assay and expansion data and indicated the superiority of culture in spinner-flask when agitated at 40 rpm over to other agitation speeds and also static culture. Cell viability and colony count was affected by changing the agitation speed. We assume that changes in cell growth resulted from the effect of shear stress directly on cell viability, and indirectly on signaling pathways that influence the cells to differentiate.
    45 schema:genre research_article
    46 schema:inLanguage en
    47 schema:isAccessibleForFree true
    48 schema:isPartOf Nb3a850764791430da139090a9789e8cf
    49 Nf69c411bb3f74ef196507024e92b2744
    50 sg:journal.1086307
    51 schema:name Agitation increases expansion of cord blood hematopoietic cells and promotes their differentiation into myeloid lineage
    52 schema:pagination 969-978
    53 schema:productId N1562cf17c40d40fdb37019611c14129c
    54 N2839051502994472876213f73daf8b2d
    55 N52412e1774394b44bd7b77a8be17646e
    56 N76671eb5170a4a57a31eeee2a6010890
    57 Nc0412f954ca74b8f9ee418a305908ca5
    58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045119257
    59 https://doi.org/10.1007/s10616-015-9851-3
    60 schema:sdDatePublished 2019-04-10T18:21
    61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    62 schema:sdPublisher N96925ed262d84c4aae7ff29bdd3e1282
    63 schema:url http://link.springer.com/10.1007%2Fs10616-015-9851-3
    64 sgo:license sg:explorer/license/
    65 sgo:sdDataset articles
    66 rdf:type schema:ScholarlyArticle
    67 N1562cf17c40d40fdb37019611c14129c schema:name pubmed_id
    68 schema:value 26264594
    69 rdf:type schema:PropertyValue
    70 N2839051502994472876213f73daf8b2d schema:name nlm_unique_id
    71 schema:value 8807027
    72 rdf:type schema:PropertyValue
    73 N52412e1774394b44bd7b77a8be17646e schema:name readcube_id
    74 schema:value d6eb064d77d21090dcaa194ed6885b4ae6debcb3bd5def5a1506f671966fce0d
    75 rdf:type schema:PropertyValue
    76 N64a8c160ec074235ab2918dadfb8f939 rdf:first sg:person.01036204527.48
    77 rdf:rest rdf:nil
    78 N76671eb5170a4a57a31eeee2a6010890 schema:name doi
    79 schema:value 10.1007/s10616-015-9851-3
    80 rdf:type schema:PropertyValue
    81 N96925ed262d84c4aae7ff29bdd3e1282 schema:name Springer Nature - SN SciGraph project
    82 rdf:type schema:Organization
    83 N9fecf39a2ee14769ad4603a06b94235e rdf:first sg:person.01161343145.70
    84 rdf:rest N64a8c160ec074235ab2918dadfb8f939
    85 Nb3a850764791430da139090a9789e8cf schema:volumeNumber 68
    86 rdf:type schema:PublicationVolume
    87 Nbe3c6d0175914485afe43c30d8d9ae1e rdf:first sg:person.015101725225.65
    88 rdf:rest N9fecf39a2ee14769ad4603a06b94235e
    89 Nc0412f954ca74b8f9ee418a305908ca5 schema:name dimensions_id
    90 schema:value pub.1045119257
    91 rdf:type schema:PropertyValue
    92 Nf69c411bb3f74ef196507024e92b2744 schema:issueNumber 4
    93 rdf:type schema:PublicationIssue
    94 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    95 schema:name Biological Sciences
    96 rdf:type schema:DefinedTerm
    97 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    98 schema:name Biochemistry and Cell Biology
    99 rdf:type schema:DefinedTerm
    100 sg:journal.1086307 schema:issn 1381-5741
    101 1573-0603
    102 schema:name Cytotechnology
    103 rdf:type schema:Periodical
    104 sg:person.01036204527.48 schema:affiliation https://www.grid.ac/institutes/grid.412553.4
    105 schema:familyName Abdekhodaie
    106 schema:givenName Mohammad J.
    107 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036204527.48
    108 rdf:type schema:Person
    109 sg:person.01161343145.70 schema:affiliation https://www.grid.ac/institutes/grid.419336.a
    110 schema:familyName Ebrahimi
    111 schema:givenName Marzieh
    112 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161343145.70
    113 rdf:type schema:Person
    114 sg:person.015101725225.65 schema:affiliation https://www.grid.ac/institutes/grid.419336.a
    115 schema:familyName Hosseinizand
    116 schema:givenName Hasti
    117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015101725225.65
    118 rdf:type schema:Person
    119 sg:pub.10.1007/bf02439320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008678274
    120 https://doi.org/10.1007/bf02439320
    121 rdf:type schema:CreativeWork
    122 sg:pub.10.1007/s10295-011-0951-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005308733
    123 https://doi.org/10.1007/s10295-011-0951-6
    124 rdf:type schema:CreativeWork
    125 sg:pub.10.1007/s10529-007-9517-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048694640
    126 https://doi.org/10.1007/s10529-007-9517-0
    127 rdf:type schema:CreativeWork
    128 sg:pub.10.1007/s10529-010-0355-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019987694
    129 https://doi.org/10.1007/s10529-010-0355-0
    130 rdf:type schema:CreativeWork
    131 sg:pub.10.1007/s10529-012-0993-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024007931
    132 https://doi.org/10.1007/s10529-012-0993-5
    133 rdf:type schema:CreativeWork
    134 sg:pub.10.1007/s10616-008-9144-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052896273
    135 https://doi.org/10.1007/s10616-008-9144-1
    136 rdf:type schema:CreativeWork
    137 sg:pub.10.1023/a:1008042000744 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038708051
    138 https://doi.org/10.1023/a:1008042000744
    139 rdf:type schema:CreativeWork
    140 sg:pub.10.1023/a:1008085932764 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020997857
    141 https://doi.org/10.1023/a:1008085932764
    142 rdf:type schema:CreativeWork
    143 sg:pub.10.1023/a:1010350215989 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032011100
    144 https://doi.org/10.1023/a:1010350215989
    145 rdf:type schema:CreativeWork
    146 sg:pub.10.1023/a:1021994026859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033211656
    147 https://doi.org/10.1023/a:1021994026859
    148 rdf:type schema:CreativeWork
    149 sg:pub.10.1038/nature04957 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047165782
    150 https://doi.org/10.1038/nature04957
    151 rdf:type schema:CreativeWork
    152 sg:pub.10.1038/nbt0393-368 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000504379
    153 https://doi.org/10.1038/nbt0393-368
    154 rdf:type schema:CreativeWork
    155 sg:pub.10.1038/nbt0994-909 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022671474
    156 https://doi.org/10.1038/nbt0994-909
    157 rdf:type schema:CreativeWork
    158 https://app.dimensions.ai/details/publication/pub.1082861925 schema:CreativeWork
    159 https://doi.org/10.1002/(sici)1097-0290(19980905)59:5<534::aid-bit2>3.0.co;2-b schema:sameAs https://app.dimensions.ai/details/publication/pub.1021766827
    160 rdf:type schema:CreativeWork
    161 https://doi.org/10.1002/(sici)1097-0290(20000620)68:6<697::aid-bit14>3.0.co;2-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017649614
    162 rdf:type schema:CreativeWork
    163 https://doi.org/10.1002/(sici)1097-0290(20000720)69:2<171::aid-bit6>3.0.co;2-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1032935299
    164 rdf:type schema:CreativeWork
    165 https://doi.org/10.1002/bit.10788 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037428194
    166 rdf:type schema:CreativeWork
    167 https://doi.org/10.1002/bit.20900 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008176312
    168 rdf:type schema:CreativeWork
    169 https://doi.org/10.1002/btpr.582 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017650432
    170 rdf:type schema:CreativeWork
    171 https://doi.org/10.1016/0167-7799(91)90145-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044696037
    172 rdf:type schema:CreativeWork
    173 https://doi.org/10.1016/j.bej.2006.11.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015058102
    174 rdf:type schema:CreativeWork
    175 https://doi.org/10.1016/j.biotechadv.2011.06.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009358353
    176 rdf:type schema:CreativeWork
    177 https://doi.org/10.1016/j.jbiotec.2006.01.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038276357
    178 rdf:type schema:CreativeWork
    179 https://doi.org/10.1016/j.jbiotec.2012.06.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028137709
    180 rdf:type schema:CreativeWork
    181 https://doi.org/10.1016/j.jim.2010.05.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014874255
    182 rdf:type schema:CreativeWork
    183 https://doi.org/10.1016/s0076-6879(06)19018-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013050371
    184 rdf:type schema:CreativeWork
    185 https://doi.org/10.1016/s0141-0229(00)00346-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032164031
    186 rdf:type schema:CreativeWork
    187 https://doi.org/10.1016/s0141-0229(03)00144-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048278333
    188 rdf:type schema:CreativeWork
    189 https://doi.org/10.1016/s0167-7799(00)01474-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045520262
    190 rdf:type schema:CreativeWork
    191 https://doi.org/10.1016/s0167-7799(03)00076-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018672050
    192 rdf:type schema:CreativeWork
    193 https://doi.org/10.1021/bp980032e schema:sameAs https://app.dimensions.ai/details/publication/pub.1015092228
    194 rdf:type schema:CreativeWork
    195 https://doi.org/10.1056/nejm198910263211707 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023228454
    196 rdf:type schema:CreativeWork
    197 https://doi.org/10.1089/ten.tec.2013.0040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059317649
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1146/annurev.bioeng.1.1.129 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044257098
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.3109/10731199.2012.712043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048849211
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.3109/21691401.2014.897630 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048598937
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.4061/2011/620247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033159527
    206 rdf:type schema:CreativeWork
    207 https://www.grid.ac/institutes/grid.412553.4 schema:alternateName Sharif University of Technology
    208 schema:name Department of Chemical and Petroleum Engineering, Sharif University of Technology, 11155-9465, Tehran, Iran
    209 rdf:type schema:Organization
    210 https://www.grid.ac/institutes/grid.419336.a schema:alternateName Royan Institute
    211 schema:name Department of Chemical and Petroleum Engineering, Sharif University of Technology, 11155-9465, Tehran, Iran
    212 Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    213 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...