The isolation and characterization of putative mesenchymal stem cells from the spiny mouse View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-10

AUTHORS

Hayley Dickinson, Phillipa Milton, Graham Jenkin

ABSTRACT

The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSCs may be isolated from different species the aim of this study was to isolate and characterize putative bone marrow derived MSCs from the spiny mouse, Acomys cahirinus. MSCs were isolated from the spiny mouse in a traditional manner, and based on plastic adherence, morphology, colony forming unit-fibroblast assays and functional assessment (adipogenic, osteogenic and chondrogenic differentiation potential) a population of putative mesenchymal stem cells from the compact bone of the spiny mouse have been isolated and characterized. Such methodological approaches overcome the lack of species-specific antibodies for the spiny mouse and could be employed for other species where the cost of generating species-specific antibodies is not warranted. More... »

PAGES

591-599

References to SciGraph publications

  • 2007-10. Isolation murine mesenchymal stem cells by positive selection in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10616-012-9443-4

    DOI

    http://dx.doi.org/10.1007/s10616-012-9443-4

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22367020


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