Controlled embryoid body formation via surface modification and avidin–biotin cross-linking View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-12

AUTHORS

David Gothard, Scott J. Roberts, Kevin M. Shakesheff, Lee D. Buttery

ABSTRACT

Cell–cell interaction is an integral part of embryoid body (EB) formation controlling 3D aggregation. Manipulation of embryonic stem (ES) cell interactions could provide control over EB formation. Studies have shown a direct relationship between EB formation and ES cell differentiation. We have previously described a cell surface modification and cross-linking method for influencing cell–cell interaction and formation of multicellular constructs. Here we show further characterisation of this engineered aggregation. We demonstrate that engineering accelerates ES cell aggregation, forming larger, denser and more stable EBs than control samples, with no significant decrease in constituent ES cell viability. However, extended culture ≥5 days reveals significant core necrosis creating a layered EB structure. Accelerated aggregation through engineering circumvents this problem as EB formation time is reduced. We conclude that the proposed engineering method influences initial ES cell-ES cell interactions and EB formation. This methodology could be employed to further our understanding of intrinsic EB properties and their effect on ES cell differentiation. More... »

PAGES

135-144

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10616-010-9255-3

DOI

http://dx.doi.org/10.1007/s10616-010-9255-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032055901

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20145998


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