Pulmonary fibrosis model using micro-CT analyzable human PSC–derived alveolar organoids containing alveolar macrophage-like cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2022-03-10

AUTHORS

Seri Choi, Jiwoong Choi, Seongmin Cheon, Jihong Song, Seung-Yeon Kim, Ji-eun Kim, Dae-Hwan Nam, Gohar Manzar, Su-Man Kim, Hyung-Sik Kang, Kee K. Kim, Sang Hoon Jeong, Ju-Han Lee, Eun-Kee Park, Minseob Lee, Hyang-Ae Lee, Ki-Suk Kim, Han-Jin Park, Won Keun Oh, Chungoo Park, Chang Hyun Lee, Eun-Mi Kim

ABSTRACT

Human lung organoids (hLOs) are useful for disease modelling and drug screening. However, a lack of immune cells in hLOs limits the recapitulation of in vivo cellular physiology. Here, we generated hLOs containing alveolar macrophage (AMφ)–like cells derived from pluripotent stem cells (PSC). To bridge hLOs with advanced human lung high-resolution X-ray computed tomography (CT), we acquired quantitative micro-CT images. Three hLO types were observed during differentiation. Among them, alveolar hLOs highly expressed not only lung epithelial cell markers but also AMφ-specific markers. Furthermore, CD68+ AMφ-like cells were spatially organized on the luminal epithelial surface of alveolar hLOs. Bleomycin-treated alveolar hLOs showed upregulated expression of fibrosis-related markers and extracellular matrix deposits in the alveolar sacs. Alveolar hLOs also showed structural alterations such as excessive tissue fraction under bleomycin treatment. Therefore, we suggest that micro-CT analyzable PSC-derived alveolar hLOs are a promising in vitro model to predict lung toxicity manifestations, including fibrosis.Graphical abstract More... »

PAGES

1-19

Journal

TITLE

Cell Biology and Toxicology

ISSUE

N/A

VOLUME

N/A

Author Affiliations

  • Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, 08826, Seoul, Republic of Korea
  • Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, School of Medicine, University of Kansas, 66160, Kansas City, KS, USA
  • School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea
  • Department of Internal Medicine, Santa Clara Valley Medical Center, 95128, San Jose, CA, USA
  • Department of Biochemistry, College of Natural Sciences, Chungnam National University, 34134, Daejeon, Republic of Korea
  • Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea
  • Immune Research Institute, Seegene Medical Foundation, 04805, Seoul, Republic of Korea
  • Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, 77025, Houston, TX, USA
  • Medical Science Research Center, Ansan Hospital, Korea University College of Medicine, 123, Jeokgeum-ro, Danwon-gu, 15355, Ansan-si, Gyeonggi, Republic of Korea
  • Department of Pathology, Ansan Hospital, Korea University College of Medicine, 123, Jeokgeum-ro, Danwon-gu, 15355, Ansan-si, Gyeonggi, Republic of Korea
  • Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University, 49267, Busan, Republic of Korea
  • Department of Radiology, Seoul National University College of Medicine, Seoul National University Hospital, 03080, Seoul, Republic of Korea
  • R&D Center for Advanced Pharmaceuticals and Evaluation, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10565-022-09698-1

    DOI

    http://dx.doi.org/10.1007/s10565-022-09698-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1146176766

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/35267148


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, 08826, Seoul, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea", 
                "Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, 08826, Seoul, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Choi", 
            "givenName": "Seri", 
            "id": "sg:person.013237166111.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013237166111.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, School of Medicine, University of Kansas, 66160, Kansas City, KS, USA", 
              "id": "http://www.grid.ac/institutes/grid.266515.3", 
              "name": [
                "Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, School of Medicine, University of Kansas, 66160, Kansas City, KS, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Choi", 
            "givenName": "Jiwoong", 
            "id": "sg:person.01005716541.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005716541.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.14005.30", 
              "name": [
                "School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cheon", 
            "givenName": "Seongmin", 
            "id": "sg:person.014074726125.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014074726125.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Internal Medicine, Santa Clara Valley Medical Center, 95128, San Jose, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.415182.b", 
              "name": [
                "Department of Internal Medicine, Santa Clara Valley Medical Center, 95128, San Jose, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Song", 
            "givenName": "Jihong", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biochemistry, College of Natural Sciences, Chungnam National University, 34134, Daejeon, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.254230.2", 
              "name": [
                "Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea", 
                "Department of Biochemistry, College of Natural Sciences, Chungnam National University, 34134, Daejeon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Seung-Yeon", 
            "id": "sg:person.012361556046.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012361556046.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.418982.e", 
              "name": [
                "Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Ji-eun", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Immune Research Institute, Seegene Medical Foundation, 04805, Seoul, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Immune Research Institute, Seegene Medical Foundation, 04805, Seoul, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nam", 
            "givenName": "Dae-Hwan", 
            "id": "sg:person.01311101437.77", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311101437.77"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, 77025, Houston, TX, USA", 
              "id": "http://www.grid.ac/institutes/grid.240145.6", 
              "name": [
                "Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, 77025, Houston, TX, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Manzar", 
            "givenName": "Gohar", 
            "id": "sg:person.01355144654.41", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355144654.41"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.14005.30", 
              "name": [
                "School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Su-Man", 
            "id": "sg:person.01302273341.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302273341.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.14005.30", 
              "name": [
                "School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kang", 
            "givenName": "Hyung-Sik", 
            "id": "sg:person.01124734133.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124734133.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biochemistry, College of Natural Sciences, Chungnam National University, 34134, Daejeon, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.254230.2", 
              "name": [
                "Department of Biochemistry, College of Natural Sciences, Chungnam National University, 34134, Daejeon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Kee K.", 
            "id": "sg:person.01110740166.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110740166.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical Science Research Center, Ansan Hospital, Korea University College of Medicine, 123, Jeokgeum-ro, Danwon-gu, 15355, Ansan-si, Gyeonggi, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.222754.4", 
              "name": [
                "Medical Science Research Center, Ansan Hospital, Korea University College of Medicine, 123, Jeokgeum-ro, Danwon-gu, 15355, Ansan-si, Gyeonggi, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jeong", 
            "givenName": "Sang Hoon", 
            "id": "sg:person.014025656527.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014025656527.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology, Ansan Hospital, Korea University College of Medicine, 123, Jeokgeum-ro, Danwon-gu, 15355, Ansan-si, Gyeonggi, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.222754.4", 
              "name": [
                "Department of Pathology, Ansan Hospital, Korea University College of Medicine, 123, Jeokgeum-ro, Danwon-gu, 15355, Ansan-si, Gyeonggi, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Ju-Han", 
            "id": "sg:person.01225012361.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225012361.53"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University, 49267, Busan, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.411144.5", 
              "name": [
                "Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University, 49267, Busan, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Park", 
            "givenName": "Eun-Kee", 
            "id": "sg:person.012243076711.97", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012243076711.97"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Radiology, Seoul National University College of Medicine, Seoul National University Hospital, 03080, Seoul, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.412484.f", 
              "name": [
                "Department of Radiology, Seoul National University College of Medicine, Seoul National University Hospital, 03080, Seoul, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Minseob", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.418982.e", 
              "name": [
                "Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Hyang-Ae", 
            "id": "sg:person.01147352455.96", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147352455.96"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "R&D Center for Advanced Pharmaceuticals and Evaluation, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.418982.e", 
              "name": [
                "R&D Center for Advanced Pharmaceuticals and Evaluation, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Ki-Suk", 
            "id": "sg:person.01141514543.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01141514543.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.418982.e", 
              "name": [
                "Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Park", 
            "givenName": "Han-Jin", 
            "id": "sg:person.01267075006.87", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267075006.87"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, 08826, Seoul, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, 08826, Seoul, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Oh", 
            "givenName": "Won Keun", 
            "id": "sg:person.01200532013.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200532013.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.14005.30", 
              "name": [
                "School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Park", 
            "givenName": "Chungoo", 
            "id": "sg:person.01224247125.36", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224247125.36"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Radiology, Seoul National University College of Medicine, Seoul National University Hospital, 03080, Seoul, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.412484.f", 
              "name": [
                "Department of Radiology, Seoul National University College of Medicine, Seoul National University Hospital, 03080, Seoul, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Chang Hyun", 
            "id": "sg:person.010322643702.41", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010322643702.41"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea", 
              "id": "http://www.grid.ac/institutes/grid.418982.e", 
              "name": [
                "Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Eun-Mi", 
            "id": "sg:person.011633275604.57", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011633275604.57"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nmeth.4448", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092055700", 
              "https://doi.org/10.1038/nmeth.4448"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41467-020-17630-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1129740330", 
              "https://doi.org/10.1038/s41467-020-17630-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-020-71293-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1132143941", 
              "https://doi.org/10.1038/s41598-020-71293-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13059-016-1033-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037189037", 
              "https://doi.org/10.1186/s13059-016-1033-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41564-018-0177-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105054530", 
              "https://doi.org/10.1038/s41564-018-0177-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12860-018-0165-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1106151423", 
              "https://doi.org/10.1186/s12860-018-0165-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm.3642", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033423157", 
              "https://doi.org/10.1038/nm.3642"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm.4430", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092568004", 
              "https://doi.org/10.1038/nm.4430"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12931-018-0864-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1106819922", 
              "https://doi.org/10.1186/s12931-018-0864-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncb3510", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085071016", 
              "https://doi.org/10.1038/ncb3510"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41596-018-0104-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1111572481", 
              "https://doi.org/10.1038/s41596-018-0104-8"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2022-03-10", 
        "datePublishedReg": "2022-03-10", 
        "description": "Human lung organoids (hLOs) are useful for disease modelling and drug screening. However, a lack of immune cells in hLOs limits the recapitulation of in vivo cellular physiology. Here, we generated hLOs containing alveolar macrophage (AM\u03c6)\u2013like cells derived from pluripotent stem cells (PSC). To bridge hLOs with advanced human lung high-resolution X-ray computed tomography (CT), we acquired quantitative micro-CT images. Three hLO types were observed during differentiation. Among them, alveolar hLOs highly expressed not only lung epithelial cell markers but also AM\u03c6-specific markers. Furthermore, CD68+ AM\u03c6-like cells were spatially organized on the luminal epithelial surface of alveolar hLOs. Bleomycin-treated alveolar hLOs showed upregulated expression of fibrosis-related markers and extracellular matrix deposits in the alveolar sacs. Alveolar hLOs also showed structural alterations such as excessive tissue fraction under bleomycin treatment. Therefore, we suggest that micro-CT analyzable PSC-derived alveolar hLOs are a promising in vitro model to predict lung toxicity manifestations, including fibrosis.Graphical abstract", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s10565-022-09698-1", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1095523", 
            "issn": [
              "0742-2091", 
              "1573-6822"
            ], 
            "name": "Cell Biology and Toxicology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }
        ], 
        "keywords": [
          "human lung organoids", 
          "pulmonary fibrosis model", 
          "fibrosis-related markers", 
          "pluripotent stem cells", 
          "epithelial cell markers", 
          "luminal epithelial surface", 
          "macrophage-like cells", 
          "immune cells", 
          "fibrosis model", 
          "alveolar organoids", 
          "bleomycin treatment", 
          "alveolar macrophages", 
          "extracellular matrix deposits", 
          "cell markers", 
          "lung organoids", 
          "upregulated expression", 
          "toxicity manifestations", 
          "epithelial surface", 
          "like cells", 
          "alveolar sacs", 
          "stem cells", 
          "markers", 
          "structural alterations", 
          "tissue fractions", 
          "cells", 
          "matrix deposits", 
          "drug screening", 
          "organoids", 
          "disease modelling", 
          "CD68", 
          "fibrosis", 
          "bleomycin", 
          "macrophages", 
          "manifestations", 
          "tomography", 
          "treatment", 
          "micro-CT images", 
          "alterations", 
          "sac", 
          "screening", 
          "cellular physiology", 
          "X-ray", 
          "expression", 
          "physiology", 
          "differentiation", 
          "lack", 
          "recapitulation", 
          "promising", 
          "fraction", 
          "types", 
          "model", 
          "images", 
          "deposits", 
          "surface", 
          "high-resolution X-ray", 
          "modelling"
        ], 
        "name": "Pulmonary fibrosis model using micro-CT analyzable human PSC\u2013derived alveolar organoids containing alveolar macrophage-like cells", 
        "pagination": "1-19", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1146176766"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s10565-022-09698-1"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "35267148"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s10565-022-09698-1", 
          "https://app.dimensions.ai/details/publication/pub.1146176766"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-06-01T22:25", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_933.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s10565-022-09698-1"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10565-022-09698-1'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10565-022-09698-1'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10565-022-09698-1'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10565-022-09698-1'


     

    This table displays all metadata directly associated to this object as RDF triples.

    336 TRIPLES      22 PREDICATES      91 URIs      72 LITERALS      5 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s10565-022-09698-1 schema:about anzsrc-for:06
    2 anzsrc-for:0601
    3 schema:author N2554211ff1ee454491dba961f376e82d
    4 schema:citation sg:pub.10.1038/ncb3510
    5 sg:pub.10.1038/nm.3642
    6 sg:pub.10.1038/nm.4430
    7 sg:pub.10.1038/nmeth.4448
    8 sg:pub.10.1038/s41467-020-17630-6
    9 sg:pub.10.1038/s41564-018-0177-8
    10 sg:pub.10.1038/s41596-018-0104-8
    11 sg:pub.10.1038/s41598-020-71293-3
    12 sg:pub.10.1186/s12860-018-0165-0
    13 sg:pub.10.1186/s12931-018-0864-2
    14 sg:pub.10.1186/s13059-016-1033-x
    15 schema:datePublished 2022-03-10
    16 schema:datePublishedReg 2022-03-10
    17 schema:description Human lung organoids (hLOs) are useful for disease modelling and drug screening. However, a lack of immune cells in hLOs limits the recapitulation of in vivo cellular physiology. Here, we generated hLOs containing alveolar macrophage (AMφ)–like cells derived from pluripotent stem cells (PSC). To bridge hLOs with advanced human lung high-resolution X-ray computed tomography (CT), we acquired quantitative micro-CT images. Three hLO types were observed during differentiation. Among them, alveolar hLOs highly expressed not only lung epithelial cell markers but also AMφ-specific markers. Furthermore, CD68+ AMφ-like cells were spatially organized on the luminal epithelial surface of alveolar hLOs. Bleomycin-treated alveolar hLOs showed upregulated expression of fibrosis-related markers and extracellular matrix deposits in the alveolar sacs. Alveolar hLOs also showed structural alterations such as excessive tissue fraction under bleomycin treatment. Therefore, we suggest that micro-CT analyzable PSC-derived alveolar hLOs are a promising in vitro model to predict lung toxicity manifestations, including fibrosis.Graphical abstract
    18 schema:genre article
    19 schema:inLanguage en
    20 schema:isAccessibleForFree true
    21 schema:isPartOf sg:journal.1095523
    22 schema:keywords CD68
    23 X-ray
    24 alterations
    25 alveolar macrophages
    26 alveolar organoids
    27 alveolar sacs
    28 bleomycin
    29 bleomycin treatment
    30 cell markers
    31 cells
    32 cellular physiology
    33 deposits
    34 differentiation
    35 disease modelling
    36 drug screening
    37 epithelial cell markers
    38 epithelial surface
    39 expression
    40 extracellular matrix deposits
    41 fibrosis
    42 fibrosis model
    43 fibrosis-related markers
    44 fraction
    45 high-resolution X-ray
    46 human lung organoids
    47 images
    48 immune cells
    49 lack
    50 like cells
    51 luminal epithelial surface
    52 lung organoids
    53 macrophage-like cells
    54 macrophages
    55 manifestations
    56 markers
    57 matrix deposits
    58 micro-CT images
    59 model
    60 modelling
    61 organoids
    62 physiology
    63 pluripotent stem cells
    64 promising
    65 pulmonary fibrosis model
    66 recapitulation
    67 sac
    68 screening
    69 stem cells
    70 structural alterations
    71 surface
    72 tissue fractions
    73 tomography
    74 toxicity manifestations
    75 treatment
    76 types
    77 upregulated expression
    78 schema:name Pulmonary fibrosis model using micro-CT analyzable human PSC–derived alveolar organoids containing alveolar macrophage-like cells
    79 schema:pagination 1-19
    80 schema:productId N60e0167056e64494a406bfd571bc5f6d
    81 N79a2850020894aeeaf7554bb82e6fe47
    82 Nf05f4d95bca54010ad920d341141a680
    83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1146176766
    84 https://doi.org/10.1007/s10565-022-09698-1
    85 schema:sdDatePublished 2022-06-01T22:25
    86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    87 schema:sdPublisher N7ae77a4fb93043e693adfde0cd1fbc76
    88 schema:url https://doi.org/10.1007/s10565-022-09698-1
    89 sgo:license sg:explorer/license/
    90 sgo:sdDataset articles
    91 rdf:type schema:ScholarlyArticle
    92 N015546d050864d658d5f621a0ce4dffd rdf:first sg:person.01147352455.96
    93 rdf:rest N6a2a844381e44a18a412f5457e4c3cb5
    94 N0cdc9a21a11e4ed5be8164fcb5fc1db6 rdf:first sg:person.01200532013.34
    95 rdf:rest Nbe402cac774a432ab4021482b88fac5a
    96 N0d0fcc8a907f47efaa3a36f9da19a782 rdf:first sg:person.01225012361.53
    97 rdf:rest Na4a786dfd70c451493bf53d4a97849e0
    98 N1e10dea4760e441aac165a731f9bf493 rdf:first sg:person.01124734133.40
    99 rdf:rest N51318116bece47bab3ad776e140ce844
    100 N2554211ff1ee454491dba961f376e82d rdf:first sg:person.013237166111.02
    101 rdf:rest Ne28e18d070c44c3288356d3bb5282eb8
    102 N33f2b4b8ea7a4e138bf37ba0300128ac schema:affiliation grid-institutes:grid.415182.b
    103 schema:familyName Song
    104 schema:givenName Jihong
    105 rdf:type schema:Person
    106 N38ff736baca8401e9013ca4e7c7a11f4 rdf:first N4cfa18ba77f7438fbe8c758e7bc6dd0f
    107 rdf:rest Nfb098ace66314b60aa97e8fd1fc5e6fb
    108 N458b7e23153543c9a5cfffb3f3258feb rdf:first N33f2b4b8ea7a4e138bf37ba0300128ac
    109 rdf:rest N4db6c8f4f3564d2183220667ece7b849
    110 N4cfa18ba77f7438fbe8c758e7bc6dd0f schema:affiliation grid-institutes:grid.418982.e
    111 schema:familyName Kim
    112 schema:givenName Ji-eun
    113 rdf:type schema:Person
    114 N4db6c8f4f3564d2183220667ece7b849 rdf:first sg:person.012361556046.35
    115 rdf:rest N38ff736baca8401e9013ca4e7c7a11f4
    116 N51318116bece47bab3ad776e140ce844 rdf:first sg:person.01110740166.45
    117 rdf:rest Nd3ea41cccf8b4f13a954ebff6f999095
    118 N60e0167056e64494a406bfd571bc5f6d schema:name doi
    119 schema:value 10.1007/s10565-022-09698-1
    120 rdf:type schema:PropertyValue
    121 N6a2a844381e44a18a412f5457e4c3cb5 rdf:first sg:person.01141514543.31
    122 rdf:rest Nc8f7aceeb252493585748779a83fc185
    123 N79a2850020894aeeaf7554bb82e6fe47 schema:name dimensions_id
    124 schema:value pub.1146176766
    125 rdf:type schema:PropertyValue
    126 N7ae77a4fb93043e693adfde0cd1fbc76 schema:name Springer Nature - SN SciGraph project
    127 rdf:type schema:Organization
    128 N7fc249bff9fe4395a84d2785eccd19a1 rdf:first sg:person.014074726125.26
    129 rdf:rest N458b7e23153543c9a5cfffb3f3258feb
    130 N8b6fdc180c194d04a46e907afee3eb3b rdf:first sg:person.010322643702.41
    131 rdf:rest Nfb84890a8d8749148c09144e3559d67e
    132 N9acae3e7ee264e0399c58b7c4dae6f71 rdf:first sg:person.01355144654.41
    133 rdf:rest Ncb46a2bad7cc481f9aec6ba0c3a5bce5
    134 Na4a786dfd70c451493bf53d4a97849e0 rdf:first sg:person.012243076711.97
    135 rdf:rest Nae37531cf4e84706abc362950f61492f
    136 Nae37531cf4e84706abc362950f61492f rdf:first Nf52fa7df56bb4768af888edc20175999
    137 rdf:rest N015546d050864d658d5f621a0ce4dffd
    138 Nbe402cac774a432ab4021482b88fac5a rdf:first sg:person.01224247125.36
    139 rdf:rest N8b6fdc180c194d04a46e907afee3eb3b
    140 Nc8f7aceeb252493585748779a83fc185 rdf:first sg:person.01267075006.87
    141 rdf:rest N0cdc9a21a11e4ed5be8164fcb5fc1db6
    142 Ncb46a2bad7cc481f9aec6ba0c3a5bce5 rdf:first sg:person.01302273341.55
    143 rdf:rest N1e10dea4760e441aac165a731f9bf493
    144 Nd3ea41cccf8b4f13a954ebff6f999095 rdf:first sg:person.014025656527.45
    145 rdf:rest N0d0fcc8a907f47efaa3a36f9da19a782
    146 Ne28e18d070c44c3288356d3bb5282eb8 rdf:first sg:person.01005716541.02
    147 rdf:rest N7fc249bff9fe4395a84d2785eccd19a1
    148 Nf05f4d95bca54010ad920d341141a680 schema:name pubmed_id
    149 schema:value 35267148
    150 rdf:type schema:PropertyValue
    151 Nf52fa7df56bb4768af888edc20175999 schema:affiliation grid-institutes:grid.412484.f
    152 schema:familyName Lee
    153 schema:givenName Minseob
    154 rdf:type schema:Person
    155 Nfb098ace66314b60aa97e8fd1fc5e6fb rdf:first sg:person.01311101437.77
    156 rdf:rest N9acae3e7ee264e0399c58b7c4dae6f71
    157 Nfb84890a8d8749148c09144e3559d67e rdf:first sg:person.011633275604.57
    158 rdf:rest rdf:nil
    159 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    160 schema:name Biological Sciences
    161 rdf:type schema:DefinedTerm
    162 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    163 schema:name Biochemistry and Cell Biology
    164 rdf:type schema:DefinedTerm
    165 sg:journal.1095523 schema:issn 0742-2091
    166 1573-6822
    167 schema:name Cell Biology and Toxicology
    168 schema:publisher Springer Nature
    169 rdf:type schema:Periodical
    170 sg:person.01005716541.02 schema:affiliation grid-institutes:grid.266515.3
    171 schema:familyName Choi
    172 schema:givenName Jiwoong
    173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005716541.02
    174 rdf:type schema:Person
    175 sg:person.010322643702.41 schema:affiliation grid-institutes:grid.412484.f
    176 schema:familyName Lee
    177 schema:givenName Chang Hyun
    178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010322643702.41
    179 rdf:type schema:Person
    180 sg:person.01110740166.45 schema:affiliation grid-institutes:grid.254230.2
    181 schema:familyName Kim
    182 schema:givenName Kee K.
    183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110740166.45
    184 rdf:type schema:Person
    185 sg:person.01124734133.40 schema:affiliation grid-institutes:grid.14005.30
    186 schema:familyName Kang
    187 schema:givenName Hyung-Sik
    188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124734133.40
    189 rdf:type schema:Person
    190 sg:person.01141514543.31 schema:affiliation grid-institutes:grid.418982.e
    191 schema:familyName Kim
    192 schema:givenName Ki-Suk
    193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01141514543.31
    194 rdf:type schema:Person
    195 sg:person.01147352455.96 schema:affiliation grid-institutes:grid.418982.e
    196 schema:familyName Lee
    197 schema:givenName Hyang-Ae
    198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147352455.96
    199 rdf:type schema:Person
    200 sg:person.011633275604.57 schema:affiliation grid-institutes:grid.418982.e
    201 schema:familyName Kim
    202 schema:givenName Eun-Mi
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011633275604.57
    204 rdf:type schema:Person
    205 sg:person.01200532013.34 schema:affiliation grid-institutes:grid.31501.36
    206 schema:familyName Oh
    207 schema:givenName Won Keun
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200532013.34
    209 rdf:type schema:Person
    210 sg:person.01224247125.36 schema:affiliation grid-institutes:grid.14005.30
    211 schema:familyName Park
    212 schema:givenName Chungoo
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224247125.36
    214 rdf:type schema:Person
    215 sg:person.012243076711.97 schema:affiliation grid-institutes:grid.411144.5
    216 schema:familyName Park
    217 schema:givenName Eun-Kee
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012243076711.97
    219 rdf:type schema:Person
    220 sg:person.01225012361.53 schema:affiliation grid-institutes:grid.222754.4
    221 schema:familyName Lee
    222 schema:givenName Ju-Han
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225012361.53
    224 rdf:type schema:Person
    225 sg:person.012361556046.35 schema:affiliation grid-institutes:grid.254230.2
    226 schema:familyName Kim
    227 schema:givenName Seung-Yeon
    228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012361556046.35
    229 rdf:type schema:Person
    230 sg:person.01267075006.87 schema:affiliation grid-institutes:grid.418982.e
    231 schema:familyName Park
    232 schema:givenName Han-Jin
    233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267075006.87
    234 rdf:type schema:Person
    235 sg:person.01302273341.55 schema:affiliation grid-institutes:grid.14005.30
    236 schema:familyName Kim
    237 schema:givenName Su-Man
    238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302273341.55
    239 rdf:type schema:Person
    240 sg:person.01311101437.77 schema:affiliation grid-institutes:None
    241 schema:familyName Nam
    242 schema:givenName Dae-Hwan
    243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311101437.77
    244 rdf:type schema:Person
    245 sg:person.013237166111.02 schema:affiliation grid-institutes:grid.31501.36
    246 schema:familyName Choi
    247 schema:givenName Seri
    248 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013237166111.02
    249 rdf:type schema:Person
    250 sg:person.01355144654.41 schema:affiliation grid-institutes:grid.240145.6
    251 schema:familyName Manzar
    252 schema:givenName Gohar
    253 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355144654.41
    254 rdf:type schema:Person
    255 sg:person.014025656527.45 schema:affiliation grid-institutes:grid.222754.4
    256 schema:familyName Jeong
    257 schema:givenName Sang Hoon
    258 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014025656527.45
    259 rdf:type schema:Person
    260 sg:person.014074726125.26 schema:affiliation grid-institutes:grid.14005.30
    261 schema:familyName Cheon
    262 schema:givenName Seongmin
    263 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014074726125.26
    264 rdf:type schema:Person
    265 sg:pub.10.1038/ncb3510 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085071016
    266 https://doi.org/10.1038/ncb3510
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1038/nm.3642 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033423157
    269 https://doi.org/10.1038/nm.3642
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1038/nm.4430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092568004
    272 https://doi.org/10.1038/nm.4430
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1038/nmeth.4448 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092055700
    275 https://doi.org/10.1038/nmeth.4448
    276 rdf:type schema:CreativeWork
    277 sg:pub.10.1038/s41467-020-17630-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1129740330
    278 https://doi.org/10.1038/s41467-020-17630-6
    279 rdf:type schema:CreativeWork
    280 sg:pub.10.1038/s41564-018-0177-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105054530
    281 https://doi.org/10.1038/s41564-018-0177-8
    282 rdf:type schema:CreativeWork
    283 sg:pub.10.1038/s41596-018-0104-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111572481
    284 https://doi.org/10.1038/s41596-018-0104-8
    285 rdf:type schema:CreativeWork
    286 sg:pub.10.1038/s41598-020-71293-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1132143941
    287 https://doi.org/10.1038/s41598-020-71293-3
    288 rdf:type schema:CreativeWork
    289 sg:pub.10.1186/s12860-018-0165-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1106151423
    290 https://doi.org/10.1186/s12860-018-0165-0
    291 rdf:type schema:CreativeWork
    292 sg:pub.10.1186/s12931-018-0864-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1106819922
    293 https://doi.org/10.1186/s12931-018-0864-2
    294 rdf:type schema:CreativeWork
    295 sg:pub.10.1186/s13059-016-1033-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037189037
    296 https://doi.org/10.1186/s13059-016-1033-x
    297 rdf:type schema:CreativeWork
    298 grid-institutes:None schema:alternateName Immune Research Institute, Seegene Medical Foundation, 04805, Seoul, Republic of Korea
    299 schema:name Immune Research Institute, Seegene Medical Foundation, 04805, Seoul, Republic of Korea
    300 rdf:type schema:Organization
    301 grid-institutes:grid.14005.30 schema:alternateName School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea
    302 schema:name School of Biological Sciences and Technology, Chonnam National University, 61186, Gwangju, Republic of Korea
    303 rdf:type schema:Organization
    304 grid-institutes:grid.222754.4 schema:alternateName Department of Pathology, Ansan Hospital, Korea University College of Medicine, 123, Jeokgeum-ro, Danwon-gu, 15355, Ansan-si, Gyeonggi, Republic of Korea
    305 Medical Science Research Center, Ansan Hospital, Korea University College of Medicine, 123, Jeokgeum-ro, Danwon-gu, 15355, Ansan-si, Gyeonggi, Republic of Korea
    306 schema:name Department of Pathology, Ansan Hospital, Korea University College of Medicine, 123, Jeokgeum-ro, Danwon-gu, 15355, Ansan-si, Gyeonggi, Republic of Korea
    307 Medical Science Research Center, Ansan Hospital, Korea University College of Medicine, 123, Jeokgeum-ro, Danwon-gu, 15355, Ansan-si, Gyeonggi, Republic of Korea
    308 rdf:type schema:Organization
    309 grid-institutes:grid.240145.6 schema:alternateName Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, 77025, Houston, TX, USA
    310 schema:name Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, 77025, Houston, TX, USA
    311 rdf:type schema:Organization
    312 grid-institutes:grid.254230.2 schema:alternateName Department of Biochemistry, College of Natural Sciences, Chungnam National University, 34134, Daejeon, Republic of Korea
    313 schema:name Department of Biochemistry, College of Natural Sciences, Chungnam National University, 34134, Daejeon, Republic of Korea
    314 Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea
    315 rdf:type schema:Organization
    316 grid-institutes:grid.266515.3 schema:alternateName Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, School of Medicine, University of Kansas, 66160, Kansas City, KS, USA
    317 schema:name Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, School of Medicine, University of Kansas, 66160, Kansas City, KS, USA
    318 rdf:type schema:Organization
    319 grid-institutes:grid.31501.36 schema:alternateName Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, 08826, Seoul, Republic of Korea
    320 schema:name Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea
    321 Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, 08826, Seoul, Republic of Korea
    322 rdf:type schema:Organization
    323 grid-institutes:grid.411144.5 schema:alternateName Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University, 49267, Busan, Republic of Korea
    324 schema:name Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University, 49267, Busan, Republic of Korea
    325 rdf:type schema:Organization
    326 grid-institutes:grid.412484.f schema:alternateName Department of Radiology, Seoul National University College of Medicine, Seoul National University Hospital, 03080, Seoul, Republic of Korea
    327 schema:name Department of Radiology, Seoul National University College of Medicine, Seoul National University Hospital, 03080, Seoul, Republic of Korea
    328 rdf:type schema:Organization
    329 grid-institutes:grid.415182.b schema:alternateName Department of Internal Medicine, Santa Clara Valley Medical Center, 95128, San Jose, CA, USA
    330 schema:name Department of Internal Medicine, Santa Clara Valley Medical Center, 95128, San Jose, CA, USA
    331 rdf:type schema:Organization
    332 grid-institutes:grid.418982.e schema:alternateName Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea
    333 R&D Center for Advanced Pharmaceuticals and Evaluation, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea
    334 schema:name Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea
    335 R&D Center for Advanced Pharmaceuticals and Evaluation, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, 34114, Daejeon, Republic of Korea
    336 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...