Overexpression of sonic hedgehog enhances the osteogenesis in rat ectomesenchymal stem cells View Full Text


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Article Info

DATE

2022-02-11

AUTHORS

Weijiang Wu, Zhe Wang, Zhijian Zhang, Wenjing Yang, Xin Fan, Jili Xu, Zhiqiang Huang, Qixiang Shao

ABSTRACT

Ectoderm-derived mesenchymal stem cells (EMSCs) were used as potential seed cells for bone tissue engineering to treat bone defects due to their capability of rapid proliferation and osteogenic differentiation. Sonic hedgehog (Shh) signaling was reported to play an important role in the development of bone tissue, but its role is not understood. The present study investigated the role of Shh molecule in osteogenic differentiation of rat EMSCs in vitro. Rat EMSCs were isolated form nasal respiratory mucosa and identified with immunofluorescence and analyzed with other methods, including reverse transcriptase polymerase chain reaction (qPCR) and western blotting. EMSCs expressed CD90, CD105, nestin, and vimentin. On the seventh day of osteogenic induction, expression levels of Shh and Gli1 was higher according to the result of qPCR and Western blotting. After induction for 14 days, higher alkaline phosphatase (ALP) activity and more mineralized nodules were seen in comparison to the cells that did not undergo induction. Shh signaling appears to enhance osteogenic differentiation of rat EMSCs, suggesting that Shh signaling directs the lineage differentiation of ectodermal stem cells and represents a promising strategy for skeletal tissue regeneration. More... »

PAGES

1-12

References to SciGraph publications

  • 2015-04-02. Ptch1 and Gli regulate Shh signalling dynamics via multiple mechanisms in NATURE COMMUNICATIONS
  • 2020-11-27. Enhanced neural differentiation of neural stem cells by sustained release of Shh from TG2 gene-modified EMSC co-culture in vitro in AMINO ACIDS
  • 2019-08-19. Stem cell-based bone and dental regeneration: a view of microenvironmental modulation in INTERNATIONAL JOURNAL OF ORAL SCIENCE
  • 2016-04-16. Mesenchymal stem cells (MSCs) as skeletal therapeutics–an update in JOURNAL OF BIOMEDICAL SCIENCE
  • 2004-10-28. Sonic hedgehog gene-enhanced tissue engineering for bone regeneration in GENE THERAPY
  • 2020-12-07. Functional crosstalk between myeloid Foxo1–β-catenin axis and Hedgehog/Gli1 signaling in oxidative stress response in CELL DEATH & DIFFERENTIATION
  • 2017-09-08. LNGFR targets the Wnt/β-catenin pathway and promotes the osteogenic differentiation in rat ectomesenchymal stem cells in SCIENTIFIC REPORTS
  • 2015-05-29. The Hedgehog signalling pathway in bone formation in INTERNATIONAL JOURNAL OF ORAL SCIENCE
  • 2008-03-19. Integration of growth and specification in chick wing digit-patterning in NATURE
  • 2020-06-26. GLI2-Mediated Inflammation in the Tumor Microenvironment in TUMOR MICROENVIRONMENT
  • 2013-11-30. Identification of Rat Respiratory Mucosa Stem Cells and Comparison of the Early Neural Differentiation Potential with the Bone Marrow Mesenchymal Stem Cells In Vitro in CELLULAR AND MOLECULAR NEUROBIOLOGY
  • 2021-03-26. Functional tissue-engineered bone-like graft made of a fibrin scaffold and TG2 gene-modified EMSCs for bone defect repair in NPG ASIA MATERIALS
  • 2011-03-09. Hedgehog/Wnt feedback supports regenerative proliferation of epithelial stem cells in bladder in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10561-022-09994-4

    DOI

    http://dx.doi.org/10.1007/s10561-022-09994-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1145465410

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/35147838


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