Potential of a novel scaffold composed of human platelet lysate and fibrin for human corneal endothelial cells View Full Text


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Article Info

DATE

2021-05-03

AUTHORS

Mohammad Amir Mishan, Sahar Balagholi, Tahereh Chamani, Sepehr Feizi, Zahra-Soheila Soheili, Mozhgan Rezaei Kanavi

ABSTRACT

Cell-based therapies have been emerged to find innovative solutions for corneal endothelial dysfunction. The aim of this study is to investigate the suitability of a blended scaffold containing human platelet lysate (HPL) and fibrin not only for cultivating human corneal endothelial cells (HCECs) but also for serving as a scaffold for the respected cells. We isolated HCECs from human donors and encapsulated the cells with three concentrations of HPL/Fibrin scaffold, namely HPL/Fibrin 1, HPL/Fibrin 2 and HPL/Fibrin 3, by adding 28.9, 57.8 and 86.7 mg/dl of fibrinogen to HPL to obtain a final percentage of 10, 20 and 30 % of fibrinogen, respectively. SEM imaging and swelling test were done to characterize the scaffolds. Cell viability assay and cell counting were performed on the cells. HCECs were characterized by morphology and immunocytochemistry. SEM imaging on freeze-dried scaffolds showed higher porosity of HPL/Fibrin 1 and HPL/Fibrin 2 than HPL/Fibrin 3, but larger pores were observed only in HPL/Fibrin 1. Cellular attachment and morphology on HPL/Fibrin 1 were appropriate by SEM imaging. A higher swelling rate was observed in HPL/Fibrin 1. After 3 and 5 days, higher numbers of cells were observed specifically in HPL/Fibrin 1. A higher expression of Na+/K+-ATPase, ZO-1 and vimentin proteins was detected in the HPL/Fibrin 1-cultured HCECs as compared with control (no scaffold). HPL/Fibrin can be used as a suitable scaffold for HCECs while preserving the cells viability. Further investigations are necessitated to approve the beneficial effects of the suggested scaffold for delivering and transplantation of cultivated HCECs into the anterior chamber of the eye. More... »

PAGES

171-183

References to SciGraph publications

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  • 2016-01-28. Erratum to: Differentiation of Human Endometrial Stem Cells into Schwann Cells in Fibrin Hydrogel as 3D Culture in MOLECULAR NEUROBIOLOGY
  • 2019-09-04. Poly-ε-lysine based hydrogels as synthetic substrates for the expansion of corneal endothelial cells for transplantation in JOURNAL OF MATERIALS SCIENCE: MATERIALS IN MEDICINE
  • 2017-11-23. Differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitor in BIOMEDICAL ENGINEERING ONLINE
  • 2015-12-19. Differentiation of Human Endometrial Stem Cells into Schwann Cells in Fibrin Hydrogel as 3D Culture in MOLECULAR NEUROBIOLOGY
  • 2013-03-21. Encapsulation and 3D culture of human adipose-derived stem cells in an in-situ crosslinked hybrid hydrogel composed of PEG-based hyperbranched copolymer and hyaluronic acid in STEM CELL RESEARCH & THERAPY
  • 1990-04. Transformation of human corneal endothelial cells by microinjection of oncogenes in BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
  • 2009-10-10. Fibrin Sealant: Past, Present, and Future: A Brief Review in WORLD JOURNAL OF SURGERY
  • 2016-05-18. Rho kinase inhibitor enables cell-based therapy for corneal endothelial dysfunction in SCIENTIFIC REPORTS
  • 2019-11-25. Autologous protein-based scaffold composed of platelet lysate and aminated hyaluronic acid in JOURNAL OF MATERIALS SCIENCE: MATERIALS IN MEDICINE
  • 2019-05-11. Trephine- and dye-free technique for eye bank preparation of pre-stripped Descemet membrane endothelial keratoplasty tissue in CELL AND TISSUE BANKING
  • 2019-04-15. Functional Evaluation of Two Corneal Endothelial Cell-Based Therapies: Tissue-Engineered Construct and Cell Injection in SCIENTIFIC REPORTS
  • 2018-11-24. Fibrin-Based Biomaterial Applications in Tissue Engineering and Regenerative Medicine in BIOMIMETIC MEDICAL MATERIALS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10561-021-09931-x

    DOI

    http://dx.doi.org/10.1007/s10561-021-09931-x

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/33939123


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