Considering 3D topography of endothelial folds to improve cell count of organ cultured corneas View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-04-10

AUTHORS

Clotilde Jumelle, Thibaud Garcin, Anne Sophie Gauthier, Yaël Glasson, Aurélien Bernard, Yann Gavet, Jacques Klossa, Zhiguo He, Sophie Acquart, Philippe Gain, Gilles Thuret

ABSTRACT

The posterior side of the cornea is covered by the endothelial monolayer, which governs corneal transparency but cannot proliferate. Determination of endothelial cell density (ECD) is therefore the minimal and mandatory quality control in all eye banks. It avoids primary graft failures caused by endothelial insufficiency, and allows allocation of corneas to surgical techniques requiring different numbers of endothelial cells (ECs). Corneas stored in organ culture (17% of grafts worldwide), are characterized by heavy stromal swelling and numerous deep endothelial folds, up to 200 µm high. During microscopic en face observation, flat surfaces are thus exceptional and EC counting is biased by parallax errors, resulting in overestimated eye bank ECD (ebECD). We used a motorized transmitted light microscope to acquire Z-stacks of images every 10 µm, and processed them to reconstruct the 3D surface of the folded endothelium. This method (3D-ECD) takes into account the local point-by-point slope in order to correct ECD. On a set of 30 corneas, we compared 3D-ECD and ebECD determined on five identical zones at the center of the cornea. 3D reconstruction allowed us to visualize twice as many cells, and ebECD was 8.1 ± 4.5% (95%CI 6.4–9.7) higher than 3D-ECD, with 1744 ± 488 versus 1606 ± 473 cells/mm2. 3D counting makes it possible to increase cell sampling and to correct overestimation by the conventional en face counting still routinely performed in eye banks. More... »

PAGES

185-191

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10561-017-9624-7

DOI

http://dx.doi.org/10.1007/s10561-017-9624-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084764017

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28397149


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1113", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Ophthalmology and Optometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Count", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cornea", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endothelium, Corneal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Eye Banks", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Imaging, Three-Dimensional", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Organ Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Organ Preservation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Quality Control", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France", 
          "id": "http://www.grid.ac/institutes/grid.6279.a", 
          "name": [
            "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jumelle", 
        "givenName": "Clotilde", 
        "id": "sg:person.01270545645.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270545645.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France", 
          "id": "http://www.grid.ac/institutes/grid.6279.a", 
          "name": [
            "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Garcin", 
        "givenName": "Thibaud", 
        "id": "sg:person.011713516365.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011713516365.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France", 
          "id": "http://www.grid.ac/institutes/grid.6279.a", 
          "name": [
            "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gauthier", 
        "givenName": "Anne Sophie", 
        "id": "sg:person.01324663540.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324663540.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France", 
          "id": "http://www.grid.ac/institutes/grid.6279.a", 
          "name": [
            "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Glasson", 
        "givenName": "Ya\u00ebl", 
        "id": "sg:person.015754352133.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015754352133.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France", 
          "id": "http://www.grid.ac/institutes/grid.6279.a", 
          "name": [
            "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bernard", 
        "givenName": "Aur\u00e9lien", 
        "id": "sg:person.01062614224.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01062614224.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ecole Nationale Sup\u00e9rieure des Mines de Saint-Etienne, Saint-Etienne, France", 
          "id": "http://www.grid.ac/institutes/grid.424462.2", 
          "name": [
            "Ecole Nationale Sup\u00e9rieure des Mines de Saint-Etienne, Saint-Etienne, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gavet", 
        "givenName": "Yann", 
        "id": "sg:person.01051243033.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051243033.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Trybvn, Ch\u00e2tillon, France", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Trybvn, Ch\u00e2tillon, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Klossa", 
        "givenName": "Jacques", 
        "id": "sg:person.0626070723.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626070723.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France", 
          "id": "http://www.grid.ac/institutes/grid.6279.a", 
          "name": [
            "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "He", 
        "givenName": "Zhiguo", 
        "id": "sg:person.0655600650.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0655600650.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Eye Bank, French Blood Center, Saint-\u00c9tienne, France", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Eye Bank, French Blood Center, Saint-\u00c9tienne, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Acquart", 
        "givenName": "Sophie", 
        "id": "sg:person.0756777566.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756777566.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Ophthalmology, University Hospital of Saint-Etienne, Saint-Etienne, France", 
          "id": "http://www.grid.ac/institutes/grid.412954.f", 
          "name": [
            "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France", 
            "Department of Ophthalmology, University Hospital of Saint-Etienne, Saint-Etienne, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gain", 
        "givenName": "Philippe", 
        "id": "sg:person.01320337664.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320337664.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Universitaire de France, Bd Saint Michel, Paris, France", 
          "id": "http://www.grid.ac/institutes/grid.440891.0", 
          "name": [
            "Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-\u00c9tienne Cedex 2, France", 
            "Department of Ophthalmology, University Hospital of Saint-Etienne, Saint-Etienne, France", 
            "Institut Universitaire de France, Bd Saint Michel, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thuret", 
        "givenName": "Gilles", 
        "id": "sg:person.01366453064.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366453064.75"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2017-04-10", 
    "datePublishedReg": "2017-04-10", 
    "description": "The posterior side of the cornea is covered by the endothelial monolayer, which governs corneal transparency but cannot proliferate. Determination of endothelial cell density (ECD) is therefore the minimal and mandatory quality control in all eye banks. It avoids primary graft failures caused by endothelial insufficiency, and allows allocation of corneas to surgical techniques requiring different numbers of endothelial cells (ECs). Corneas stored in organ culture (17% of grafts worldwide), are characterized by heavy stromal swelling and numerous deep endothelial folds, up to 200\u00a0\u00b5m high. During microscopic en face observation, flat surfaces are thus exceptional and EC counting is biased by parallax errors, resulting in overestimated eye bank ECD (ebECD). We used a motorized transmitted light microscope to acquire Z-stacks of images every 10\u00a0\u00b5m, and processed them to reconstruct the 3D surface of the folded endothelium. This method (3D-ECD) takes into account the local point-by-point slope in order to correct ECD. On a set of 30 corneas, we compared 3D-ECD and ebECD determined on five identical zones at the center of the cornea. 3D reconstruction allowed us to visualize twice as many cells, and ebECD was 8.1\u00a0\u00b1\u00a04.5% (95%CI 6.4\u20139.7) higher than 3D-ECD, with 1744\u00a0\u00b1\u00a0488 versus 1606\u00a0\u00b1\u00a0473 cells/mm2. 3D counting makes it possible to increase cell sampling and to correct overestimation by the conventional en face counting still routinely performed in eye banks.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s10561-017-9624-7", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1023785", 
        "issn": [
          "1389-9333", 
          "1573-6814"
        ], 
        "name": "Cell and Tissue Banking", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "18"
      }
    ], 
    "keywords": [
      "endothelial cell density", 
      "eye bank", 
      "endothelial cells", 
      "primary graft failure", 
      "cells/mm2", 
      "endothelial folds", 
      "graft failure", 
      "surgical technique", 
      "endothelial insufficiency", 
      "cell count", 
      "corneal transparency", 
      "cornea", 
      "stromal swelling", 
      "endothelial monolayers", 
      "organ culture", 
      "posterior side", 
      "light microscope", 
      "allocation of corneas", 
      "cell density", 
      "cells", 
      "cell sampling", 
      "insufficiency", 
      "endothelium", 
      "organs", 
      "count", 
      "swelling", 
      "failure", 
      "folds", 
      "control", 
      "center", 
      "z-stacks", 
      "counting", 
      "culture", 
      "reconstruction", 
      "quality control", 
      "number", 
      "microscopic", 
      "sampling", 
      "side", 
      "face", 
      "overestimation", 
      "mm2", 
      "observations", 
      "technique", 
      "method", 
      "point", 
      "monolayers", 
      "face observation", 
      "determination", 
      "images", 
      "microscope", 
      "order", 
      "density", 
      "slope", 
      "identical zones", 
      "topography", 
      "surface", 
      "zone", 
      "different numbers", 
      "allocation", 
      "local point", 
      "account", 
      "error", 
      "banks", 
      "set", 
      "transparency", 
      "flat surface", 
      "point slope", 
      "parallax error"
    ], 
    "name": "Considering 3D topography of endothelial folds to improve cell count of organ cultured corneas", 
    "pagination": "185-191", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084764017"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10561-017-9624-7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28397149"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10561-017-9624-7", 
      "https://app.dimensions.ai/details/publication/pub.1084764017"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-10T10:16", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220509/entities/gbq_results/article/article_725.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s10561-017-9624-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10561-017-9624-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10561-017-9624-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10561-017-9624-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10561-017-9624-7'


 

This table displays all metadata directly associated to this object as RDF triples.

258 TRIPLES      21 PREDICATES      105 URIs      97 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10561-017-9624-7 schema:about N07349b02869c4438a225c7a696fa043f
2 N192bee042baa489da21a708487ad1769
3 N19affcae746a4d71b65bb8bffb749d40
4 N2c45df22bb194c29aa7ededb56c8bc36
5 N3cd9c632b9d84cd7903fcfc3ac0ea810
6 N3dc0355a992c40e98dddcfd5fd1b1549
7 N521c556d73e849fd81aff3c9fe0ee8ee
8 Nbe2796afeff04fdd826fbdf7b713ee4c
9 Ne2f3c95f8e59461d89ac189c4a5b17fe
10 Ned0ad141f06e41ea9178f401960fe33f
11 anzsrc-for:11
12 anzsrc-for:1113
13 schema:author N9e059ae6475b4dbc85f9c8cf013d4196
14 schema:datePublished 2017-04-10
15 schema:datePublishedReg 2017-04-10
16 schema:description The posterior side of the cornea is covered by the endothelial monolayer, which governs corneal transparency but cannot proliferate. Determination of endothelial cell density (ECD) is therefore the minimal and mandatory quality control in all eye banks. It avoids primary graft failures caused by endothelial insufficiency, and allows allocation of corneas to surgical techniques requiring different numbers of endothelial cells (ECs). Corneas stored in organ culture (17% of grafts worldwide), are characterized by heavy stromal swelling and numerous deep endothelial folds, up to 200 µm high. During microscopic en face observation, flat surfaces are thus exceptional and EC counting is biased by parallax errors, resulting in overestimated eye bank ECD (ebECD). We used a motorized transmitted light microscope to acquire Z-stacks of images every 10 µm, and processed them to reconstruct the 3D surface of the folded endothelium. This method (3D-ECD) takes into account the local point-by-point slope in order to correct ECD. On a set of 30 corneas, we compared 3D-ECD and ebECD determined on five identical zones at the center of the cornea. 3D reconstruction allowed us to visualize twice as many cells, and ebECD was 8.1 ± 4.5% (95%CI 6.4–9.7) higher than 3D-ECD, with 1744 ± 488 versus 1606 ± 473 cells/mm2. 3D counting makes it possible to increase cell sampling and to correct overestimation by the conventional en face counting still routinely performed in eye banks.
17 schema:genre article
18 schema:inLanguage en
19 schema:isAccessibleForFree false
20 schema:isPartOf N98adb071a46f4189820ca107adbefe38
21 Na12bfb855ef341dbb1d0fca08d1ecd72
22 sg:journal.1023785
23 schema:keywords account
24 allocation
25 allocation of corneas
26 banks
27 cell count
28 cell density
29 cell sampling
30 cells
31 cells/mm2
32 center
33 control
34 cornea
35 corneal transparency
36 count
37 counting
38 culture
39 density
40 determination
41 different numbers
42 endothelial cell density
43 endothelial cells
44 endothelial folds
45 endothelial insufficiency
46 endothelial monolayers
47 endothelium
48 error
49 eye bank
50 face
51 face observation
52 failure
53 flat surface
54 folds
55 graft failure
56 identical zones
57 images
58 insufficiency
59 light microscope
60 local point
61 method
62 microscope
63 microscopic
64 mm2
65 monolayers
66 number
67 observations
68 order
69 organ culture
70 organs
71 overestimation
72 parallax error
73 point
74 point slope
75 posterior side
76 primary graft failure
77 quality control
78 reconstruction
79 sampling
80 set
81 side
82 slope
83 stromal swelling
84 surface
85 surgical technique
86 swelling
87 technique
88 topography
89 transparency
90 z-stacks
91 zone
92 schema:name Considering 3D topography of endothelial folds to improve cell count of organ cultured corneas
93 schema:pagination 185-191
94 schema:productId N7d88f879503741d2a2e15f25ece637a9
95 Nc15ca892467c48408948d17ea794e6e9
96 Nce8d2e37b6794a18aaedef40f841cbad
97 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084764017
98 https://doi.org/10.1007/s10561-017-9624-7
99 schema:sdDatePublished 2022-05-10T10:16
100 schema:sdLicense https://scigraph.springernature.com/explorer/license/
101 schema:sdPublisher N90bb383c51724eb8bf8d2636fb00523a
102 schema:url https://doi.org/10.1007/s10561-017-9624-7
103 sgo:license sg:explorer/license/
104 sgo:sdDataset articles
105 rdf:type schema:ScholarlyArticle
106 N07349b02869c4438a225c7a696fa043f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Humans
108 rdf:type schema:DefinedTerm
109 N169262e2d5644c3d965042a905953bb7 rdf:first sg:person.0655600650.20
110 rdf:rest Ndf937dfd54b1474cb20fcc0ab69f7b1d
111 N192bee042baa489da21a708487ad1769 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Microscopy
113 rdf:type schema:DefinedTerm
114 N19affcae746a4d71b65bb8bffb749d40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Cell Count
116 rdf:type schema:DefinedTerm
117 N26b24aef20864473ae7cacec786143a7 rdf:first sg:person.01051243033.49
118 rdf:rest Nb78b06a799b544c99a1f74186165008c
119 N2c45df22bb194c29aa7ededb56c8bc36 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Eye Banks
121 rdf:type schema:DefinedTerm
122 N3cd9c632b9d84cd7903fcfc3ac0ea810 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Organ Culture Techniques
124 rdf:type schema:DefinedTerm
125 N3dc0355a992c40e98dddcfd5fd1b1549 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Quality Control
127 rdf:type schema:DefinedTerm
128 N521c556d73e849fd81aff3c9fe0ee8ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Imaging, Three-Dimensional
130 rdf:type schema:DefinedTerm
131 N644014477f90498e8c71169c4f8faa61 rdf:first sg:person.01062614224.12
132 rdf:rest N26b24aef20864473ae7cacec786143a7
133 N7d88f879503741d2a2e15f25ece637a9 schema:name dimensions_id
134 schema:value pub.1084764017
135 rdf:type schema:PropertyValue
136 N90bb383c51724eb8bf8d2636fb00523a schema:name Springer Nature - SN SciGraph project
137 rdf:type schema:Organization
138 N9436324f89b9483eabe12f7d3b06013e rdf:first sg:person.01366453064.75
139 rdf:rest rdf:nil
140 N98adb071a46f4189820ca107adbefe38 schema:volumeNumber 18
141 rdf:type schema:PublicationVolume
142 N9e059ae6475b4dbc85f9c8cf013d4196 rdf:first sg:person.01270545645.76
143 rdf:rest Nb3360b018d0e4b37b30d28cdb5d065a9
144 Na12bfb855ef341dbb1d0fca08d1ecd72 schema:issueNumber 2
145 rdf:type schema:PublicationIssue
146 Nac396d4625e44cb5bef55623f8b945c0 rdf:first sg:person.015754352133.23
147 rdf:rest N644014477f90498e8c71169c4f8faa61
148 Nb3360b018d0e4b37b30d28cdb5d065a9 rdf:first sg:person.011713516365.40
149 rdf:rest Nb72c1eefafec478eba8e485ebd2bb4e2
150 Nb72c1eefafec478eba8e485ebd2bb4e2 rdf:first sg:person.01324663540.15
151 rdf:rest Nac396d4625e44cb5bef55623f8b945c0
152 Nb78b06a799b544c99a1f74186165008c rdf:first sg:person.0626070723.60
153 rdf:rest N169262e2d5644c3d965042a905953bb7
154 Nbe2796afeff04fdd826fbdf7b713ee4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Organ Preservation
156 rdf:type schema:DefinedTerm
157 Nc15ca892467c48408948d17ea794e6e9 schema:name doi
158 schema:value 10.1007/s10561-017-9624-7
159 rdf:type schema:PropertyValue
160 Nce8d2e37b6794a18aaedef40f841cbad schema:name pubmed_id
161 schema:value 28397149
162 rdf:type schema:PropertyValue
163 Ndf937dfd54b1474cb20fcc0ab69f7b1d rdf:first sg:person.0756777566.66
164 rdf:rest Nf954c7cb7bd440ab932cb6877d07e508
165 Ne2f3c95f8e59461d89ac189c4a5b17fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Endothelium, Corneal
167 rdf:type schema:DefinedTerm
168 Ned0ad141f06e41ea9178f401960fe33f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Cornea
170 rdf:type schema:DefinedTerm
171 Nf954c7cb7bd440ab932cb6877d07e508 rdf:first sg:person.01320337664.46
172 rdf:rest N9436324f89b9483eabe12f7d3b06013e
173 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
174 schema:name Medical and Health Sciences
175 rdf:type schema:DefinedTerm
176 anzsrc-for:1113 schema:inDefinedTermSet anzsrc-for:
177 schema:name Ophthalmology and Optometry
178 rdf:type schema:DefinedTerm
179 sg:journal.1023785 schema:issn 1389-9333
180 1573-6814
181 schema:name Cell and Tissue Banking
182 schema:publisher Springer Nature
183 rdf:type schema:Periodical
184 sg:person.01051243033.49 schema:affiliation grid-institutes:grid.424462.2
185 schema:familyName Gavet
186 schema:givenName Yann
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051243033.49
188 rdf:type schema:Person
189 sg:person.01062614224.12 schema:affiliation grid-institutes:grid.6279.a
190 schema:familyName Bernard
191 schema:givenName Aurélien
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01062614224.12
193 rdf:type schema:Person
194 sg:person.011713516365.40 schema:affiliation grid-institutes:grid.6279.a
195 schema:familyName Garcin
196 schema:givenName Thibaud
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011713516365.40
198 rdf:type schema:Person
199 sg:person.01270545645.76 schema:affiliation grid-institutes:grid.6279.a
200 schema:familyName Jumelle
201 schema:givenName Clotilde
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270545645.76
203 rdf:type schema:Person
204 sg:person.01320337664.46 schema:affiliation grid-institutes:grid.412954.f
205 schema:familyName Gain
206 schema:givenName Philippe
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320337664.46
208 rdf:type schema:Person
209 sg:person.01324663540.15 schema:affiliation grid-institutes:grid.6279.a
210 schema:familyName Gauthier
211 schema:givenName Anne Sophie
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324663540.15
213 rdf:type schema:Person
214 sg:person.01366453064.75 schema:affiliation grid-institutes:grid.440891.0
215 schema:familyName Thuret
216 schema:givenName Gilles
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366453064.75
218 rdf:type schema:Person
219 sg:person.015754352133.23 schema:affiliation grid-institutes:grid.6279.a
220 schema:familyName Glasson
221 schema:givenName Yaël
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015754352133.23
223 rdf:type schema:Person
224 sg:person.0626070723.60 schema:affiliation grid-institutes:None
225 schema:familyName Klossa
226 schema:givenName Jacques
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626070723.60
228 rdf:type schema:Person
229 sg:person.0655600650.20 schema:affiliation grid-institutes:grid.6279.a
230 schema:familyName He
231 schema:givenName Zhiguo
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0655600650.20
233 rdf:type schema:Person
234 sg:person.0756777566.66 schema:affiliation grid-institutes:None
235 schema:familyName Acquart
236 schema:givenName Sophie
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756777566.66
238 rdf:type schema:Person
239 grid-institutes:None schema:alternateName Eye Bank, French Blood Center, Saint-Étienne, France
240 Trybvn, Châtillon, France
241 schema:name Eye Bank, French Blood Center, Saint-Étienne, France
242 Trybvn, Châtillon, France
243 rdf:type schema:Organization
244 grid-institutes:grid.412954.f schema:alternateName Department of Ophthalmology, University Hospital of Saint-Etienne, Saint-Etienne, France
245 schema:name Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-Étienne Cedex 2, France
246 Department of Ophthalmology, University Hospital of Saint-Etienne, Saint-Etienne, France
247 rdf:type schema:Organization
248 grid-institutes:grid.424462.2 schema:alternateName Ecole Nationale Supérieure des Mines de Saint-Etienne, Saint-Etienne, France
249 schema:name Ecole Nationale Supérieure des Mines de Saint-Etienne, Saint-Etienne, France
250 rdf:type schema:Organization
251 grid-institutes:grid.440891.0 schema:alternateName Institut Universitaire de France, Bd Saint Michel, Paris, France
252 schema:name Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-Étienne Cedex 2, France
253 Department of Ophthalmology, University Hospital of Saint-Etienne, Saint-Etienne, France
254 Institut Universitaire de France, Bd Saint Michel, Paris, France
255 rdf:type schema:Organization
256 grid-institutes:grid.6279.a schema:alternateName Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-Étienne Cedex 2, France
257 schema:name Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 10, Rue de la Marandiere, 42023, Saint-Étienne Cedex 2, France
258 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...