Genomic screening for genes upregulated by demethylation revealed novel targets of epigenetic silencing in breast cancer View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-10-27

AUTHORS

Tomoko Fujikane, Noriko Nishikawa, Minoru Toyota, Hiromu Suzuki, Masanori Nojima, Reo Maruyama, Masami Ashida, Mutsumi Ohe-Toyota, Masahiro Kai, Toshihiko Nishidate, Yasushi Sasaki, Tousei Ohmura, Koichi Hirata, Takashi Tokino

ABSTRACT

Breast cancer arises through the accumulation of multiple genetic alterations and epigenetic changes such as methylation, which silences gene expression in a variety of cancers. In the present study, we applied genomic screening to identify genes upregulated by the demethylating agent 5-aza-2′-deoxycytidine (DAC) in a human breast cancer cell line (MCF7). We identified 288 genes upregulated and 29 genes downregulated more than fivefold after treatment with DAC, and gene ontology analyses revealed the genes to be involved in immune responses, apoptosis, and cell differentiation. In addition, real-time PCR analysis of ten genes silenced in MCF7 cells confirmed that they are upregulated by DAC, while bisulfite-pyrosequencing analysis confirmed that nine of those genes were silenced by methylation. We also found that treating MCF7 cells with DAC restored induction of DFNA5 by p53, as well as by two other p53 family genes, p63γ and p73β. Introduction of NTN4 into MCF7 cells suppressed cell growth, indicating that NTN4 has tumor suppressive activity. In primary breast cancers, we detected cancer-specific methylation of NTN4, PGP9.5, and DKK3, suggesting that methylation of these genes could be useful markers for diagnosis of breast cancer. Thus, DNA methylation appears to be a common event in breast cancer, and the genes silenced by methylation could be useful targets for both diagnosis and therapy. More... »

PAGES

699-710

References to SciGraph publications

  • 2001-09-01. Adenovirus-mediated transfer of the p53 family genes, p73 and p51/p63 induces cell cycle arrest and apoptosis in colorectal cancer cell lines: potential application to gene therapy of colorectal cancer in GENE THERAPY
  • 1998-10. Nonsyndromic hearing impairment is associated with a mutation in DFNA5 in NATURE GENETICS
  • 2006-08-02. The potential role of DFNA5, a hearing impairment gene, in p53-mediated cellular response to DNA damage in JOURNAL OF HUMAN GENETICS
  • 2006-01-30. Aberrant methylation of the Wnt antagonist SFRP1 in breast cancer is associated with unfavourable prognosis in ONCOGENE
  • 2009-02-27. Methylation of homeobox genes is a frequent and early epigenetic event in breast cancer in BREAST CANCER RESEARCH
  • 2008-09-30. Wnt signalling in human breast cancer: expression of the putative Wnt inhibitor Dickkopf-3 (DKK3) is frequently suppressed by promoter hypermethylation in mammary tumours in BREAST CANCER RESEARCH
  • 2004-03-14. Epigenetic inactivation of SFRP genes allows constitutive WNT signaling in colorectal cancer in NATURE GENETICS
  • 2008-02-19. Frequent epigenetic inactivation of Wnt antagonist genes in breast cancer in BRITISH JOURNAL OF CANCER
  • 2007-02-05. Frequent epigenetic inactivation of SFRP genes and constitutive activation of Wnt signaling in gastric cancer in ONCOGENE
  • 2008-04-09. Williams–Beuren syndrome TRIM50 encodes an E3 ubiquitin ligase in EUROPEAN JOURNAL OF HUMAN GENETICS
  • 2004-12. Netrin-1 and its receptors in tumorigenesis in NATURE REVIEWS CANCER
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10549-009-0600-1

    DOI

    http://dx.doi.org/10.1007/s10549-009-0600-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1025506697

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19859801


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