Alteration of expression levels of the oxidative phosphorylation system (OXPHOS) in breast cancer cell mitochondria View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-09-26

AUTHORS

Lorenza Putignani, Salvatore Raffa, Roberta Pescosolido, Laura Aimati, Fabrizio Signore, Maria Rosaria Torrisi, Paola Grammatico

ABSTRACT

Mitochondria are dynamic intracellular organelles playing a central role in cell metabolism by generating ATP, through the oxidative phosphorylation system (OXPHOS). Altered mitochondrial functions have been identified as causative or contributing factors in some degenerative diseases and are becoming crucial to understanding cancer mechanisms. We report on distinct expression differences between mitochondria of normal and breast-infiltrating ductal carcinoma (IDC) cells. Mitochondria isolated from HMC (human mammary carcinoma) and HMEC (human mammary epithelial cell) cultures were assayed for expression levels of the multi-protein OXPHOS complexes using Western blot and densitometric analyses. Depressed expression levels were detected for all HMC OXPHOS complexes. Drastic signal reduction was observed for the succinate-dehydrogenase complex II iron-sulphur protein SDH-B (3.38%), while decreasing was reported for the NADH-ubiquinone oxidoreductase complex I Fe-S protein 3 NDUFS3 (32.78%) and the ubiquinol-cytochrome c reductase complex III protein 2 UQCRC2 (50.34%). A significant signal dropping was detected for the ATP-synthase complex V F1β subunit (18.07%). For the cytochrome-oxidase complex IV (CO), near-depletion of the mitochondrial-encoded COI (4.37%) and no apparent variation of the COIV (97.26%) subunits were observed. CO and ATP-synthase were also assayed by cryo-immunoelectron microscopy (CIEM) on unfractionated HMC and HEMC cell mitochondria. COI and F1β differential expression, invariance of COIV levels were corroborated, while HMC mitochondria morphology deterioration was highlighted. MitoTracker Red and fluorescence immunolabelling merging confirmed CIEM data. MitoTracker Red and Green co-staining showed mitochondria membrane property modulation. These data describe bioenergetic and phenotypic alterations of IDC cell mitochondria, possibly providing new cancer hallmarks. More... »

PAGES

439-452

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10549-007-9738-x

DOI

http://dx.doi.org/10.1007/s10549-007-9738-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031670808

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17899367


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109 mitochondrial-encoded COI
110 modulation
111 morphology deterioration
112 multi-protein OXPHOS complexes
113 organelles
114 oxidative phosphorylation system
115 oxidoreductase complex I Fe
116 phenotypic alterations
117 phosphorylation system
118 property modulation
119 protein 2 UQCRC2
120 protein 3 NDUFS3
121 protein SDH
122 red
123 reductase
124 reduction
125 role
126 signal dropping
127 signal reduction
128 significant signal dropping
129 subunits
130 system
131 unfractionated HMC
132 variation
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