Increasement of O-acetylhomoserine production in Escherichia coli by modification of glycerol-oxidative pathway coupled with optimization of fermentation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2020-10-20

AUTHORS

Peng Liu, Ji-Song Liu, Bo Zhang, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

ObjectiveO-acetylhomoserine (OAH) is an important platform chemical to produce high-valuable chemicals. To improve the production of O-acetylhomoserine from glycerol, the glycerol-oxidative pathway was investigated and the optimization of fermentation with crude glycerol was carried out.ResultsThe glycerol-uptake system and glycerol-oxidative pathway were modified and O-acetyltransferase from Corynebacterium glutamicum was introduced into the engineered strain to produce O-acetylhomoserine. It was found that overexpression of glycerol 3-phosphate dehydrogenase improved the OAH production to 6.79 and 4.21 g/L from pure and crude glycerol, respectively. And the higher OAH production depending on higher level of transcription of glpD. Two-step statistical approach was employed to optimize the fermentation conditions. The significant effects of glycerol, ammonium chloride and yeast extract were screened applying Plackett–Burman design and were optimized further by employing the Response Surface Methodology. Under optimized conditions, the OAH production was up to 9.42 and 7.01 g/L when pure and crude glycerol were used in shake flask cultivations, respectively.ConclusionsThe enzymatic step catalyzing the oxidation of glycerol through GlpD was the key step for OAH production, which served the foundation for realization of a consistent OAH production from crude glycerol in the future. More... »

PAGES

105-117

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-020-03031-8

DOI

http://dx.doi.org/10.1007/s10529-020-03031-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1131926901

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33083859


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